scholarly journals Characterisation of nerve‐mediated ATP release from bladder detrusor muscle and its pathological implications

2019 ◽  
Vol 176 (24) ◽  
pp. 4720-4730 ◽  
Author(s):  
Carly J. McCarthy ◽  
Youko Ikeda ◽  
Deborah Skennerton ◽  
Basu Chakrabarty ◽  
Anthony J. Kanai ◽  
...  
Keyword(s):  
Atp Release ◽  
Detrusor Muscle ◽  
Bladder Detrusor

Alterations in neurogenically mediated contractile responses of urinary bladder in rats with diabetes

2005 ◽  
Vol 288 (6) ◽  
pp. F1220-F1226 ◽  
Author(s):  
Guiming Liu ◽  
Firouz Daneshgari
Keyword(s):  
Urinary Bladder ◽  
Unknown Origin ◽  
Electrical Field Stimulation ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Detrusor Muscle ◽  
Sprague Dawley ◽  
Contractile Responses ◽  
Bladder Detrusor ◽  
Induced Changes

Diabetic bladder dysfunction (DBD) is among the most common and bothersome complications of diabetes mellitus. Autonomic neuropathy has been counted as the cause of DBD. In the present study, we compared the alterations in the neurogenically mediated contractile responses of urinary bladder in rats with streptozocin-induced diabetes, 5% sucrose-induced diuresis, and age-matched controls. Male Sprague-Dawley rats were divided into three groups: 9-wk diabetic rats, diuretic rats, and age-matched controls. Micturition and morphometric characteristics were evaluated using metabolic cage and gross examination of the bladder. Bladder detrusor muscle strips were exposed to either periodic electrical field stimulation (EFS) or to EFS in the presence of atropine, α,β-methylene adrenasine 5′-triphosphate, or tetrodotoxin. The proportions of cholinergic, purinergic, and residual nonadrenergic-noncholinergic (NANC) components of contractile response were compared among the three groups of animals. Diabetes caused a significant reduction of body weight compared with diuresis and controls, although the bladders of diabetic and diuretic rats weighed more than the controls. Both diabetes and diuresis caused significant increase in fluid intake, urine output, and bladder size. Diabetes and diuresis caused similarly increased response to EFS and reduced response to cholinergic component compared with controls. However, the purinergic response was significantly smaller in diuretic bladder strips compared with controls but not in diabetic rats. A residual NANC of unknown origin increased significantly but differently in diabetics and diuretics compared with controls. In conclusion, neurogenically mediated bladder contraction is altered in the diabetic rat. Diabetic-related changes do not parallel diuretic-induced changes, indicating that the pathogenesis of DBD needs further exploration.

Role of Calcium Ion Antagonists of the Bladder Detrusor Muscle: In Vitro and in Vivo Study

1986 ◽  
Vol 135 (6) ◽  
pp. 1327-1331 ◽  
Author(s):  
M. Hassouna ◽  
O. Nishizawa ◽  
I. Miyagawa ◽  
A. Toguri ◽  
M. Gotoh ◽  
...  
Keyword(s):  
Calcium Ion ◽  
In Vivo Study ◽  
Detrusor Muscle ◽  
Bladder Detrusor

Selective sacral rootlet rhizotomy for hypertonic neurogenic bladder

1975 ◽  
Vol 42 (5) ◽  
pp. 567-574 ◽  
Author(s):  
Stanislaw K. Toczek ◽  
David C. McCullough ◽  
Guy W. Gargour ◽  
Rudolf Kachman ◽  
Roger Baker ◽  
...  
Keyword(s):  
Neurogenic Bladder ◽  
Conus Medullaris ◽  
Physiological Data ◽  
Detrusor Muscle ◽  
Lower Extremity Function ◽  
Accurate Identification ◽  
Content Type ◽  
Alternative Pathways ◽  
Bladder Detrusor ◽  
Stimulation Of

✓ The authors describe a highly selective transsacral microsurgical procedure for sacral nerve rootlet interruption in five patients with hypertonic neurogenic bladder. Magnification and systematic stimulation of sacral roots provided accurate identification of motor fibers supplying bladder detrusor muscle and differentiation of efferent components to the legs and anal sphincter. Although the technique prevented incontinence and adverse effects of nerve section on rectal and lower extremity function, improvement in voiding patterns and diminution of urinary sepsis was of brief duration in three of the five patients. Physiological data from these procedures reaffirms the importance of S-3 and S-4 motor roots in detrusor innervation, but clinical responses bring into question the possibility of sustained improvement from such a highly selective procedure at the sacral level. The authors suggest that alternative pathways, not apparent on initial stimulation, may develop after section of sacral root components, and that dissection and stimulation of fibers at the level of the conus medullaris should be investigated as an alternative procedure.

scholarly journals Safety and efficacy of en bloc transurethral resection versus conventional transurethral resection for primary nonmuscle-invasive bladder cancer: a meta-analysis

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Dong Zhang ◽  
Lifeng Yao ◽  
Sui Yu ◽  
Yue Cheng ◽  
Junhui Jiang ◽  
...  
Keyword(s):  
Recurrence Rate ◽  
Transurethral Resection ◽  
Bladder Tumor ◽  
Meta Analysis ◽  
Residual Tumor ◽  
Detrusor Muscle ◽  
En Bloc ◽  
Safety And Efficacy ◽  
Significant Difference ◽  
Bladder Detrusor

Abstract Background The purpose of this meta-analysis is to compare the safety and efficacy of en bloc transurethral resection of bladder tumor (EBRT) versus conventional transurethral resection of bladder tumor (CTURBT). Methods We performed a meta-analysis of relevant articles through November 2019 using PubMed, Embase, and Cochrane Central Register to compare the safety and efficacy of EBRT versus CTURBT. The main endpoint included the operation time (OT), hospitalization time (HT), catheterization time (AT), perioperative period complications, bladder detrusor muscle found in the specimen, the residual tumor on the base, the ratio of the same site recurrence, and 12/24/36-month recurrence rate. Cochrane Collaboration’s Revman software, version 5.3, was used for statistical analysis. Results A total of 19 studies with 2651 patients were included, 1369 underwent EBRT and 1282 underwent CTURBT. Patients treated with EBRT had a significantly lower AT, HT, obturator nerve reflex, bladder perforation, bladder irritation, postoperative complications, and 24-month recurrence rate than those who underwent CTURBT. While no significant difference was found in terms of OT, the ratio of bladder detrusor muscle found in the specimen, the residual tumor on the base, 12-month recurrence rate, 36-month recurrence rate, and the ratio of the same site recurrence. In mitomycin subgroup, EBRT was superior to CTURBT in terms of 12/24-month recurrence rate. Similarly, in the prospective subgroup and retrospective subgroup, EBRT had a lower 24-month recurrence rate than CTURBT. However, no significant difference was found in the low, intermediate, and high-risk group in the light of 12–36-month recurrence rate. Conclusions Based on the included 19 articles, EBRT had a significantly lower AT, HT, intraoperative and postoperative complications, and 24-month recurrence rate than those treated with CTURBT. Well-designed randomized controlled trials were needed to reevaluate these outcomes. Trial registration This meta-analysis was reported in agreement with the PRISMA statement and was registered on PROSPERO 2019 CRD42019121673.

scholarly journals Porcine Bladder Urothelial, Myofibroblast, and Detrusor Muscle Cells: Characterization and ATP Release

2011 ◽  
Vol 2 ◽  
Author(s):  
Ying Cheng ◽  
Kylie J. Mansfield ◽  
Shaun L. Sandow ◽  
Prajni Sadananda ◽  
Elizabeth Burcher ◽  
...  
Keyword(s):  
Atp Release ◽  
Muscle Cells ◽  
Detrusor Muscle

β-NAD is a novel nucleotide released on stimulation of nerve terminals in human urinary bladder detrusor muscle

2006 ◽  
Vol 290 (2) ◽  
pp. F486-F495 ◽  
Author(s):  
Leanne T. Breen ◽  
Lisa M. Smyth ◽  
Ilia A. Yamboliev ◽  
Violeta N. Mutafova-Yambolieva
Keyword(s):  
Urinary Bladder ◽  
Neural Control ◽  
Electrical Field Stimulation ◽  
Specific Substrate ◽  
Detrusor Muscle ◽  
Hplc Fraction ◽  
Human Urinary Bladder ◽  
Adp Ribosyl Cyclase ◽  
Bladder Detrusor ◽  
Spontaneous Contractions

Endogenous nucleotides with extracellular functions may be involved in the complex neural control of human urinary bladder (HUB). Using HPLC techniques with fluorescence detection, we observed that in addition to ATP and its metabolites ADP, AMP and adenosine, electrical field stimulation (EFS; 4–16 Hz, 0.1 ms, 15 V, 60 s) of HUB detrusor smooth muscle coreleases novel nucleotide factors, which produce etheno-1 N6-ADP-ribose (eADPR) on etheno-derivatization at high temperature. A detailed HPLC fraction analysis determined that nicotinamide adenine dinucleotide (β-NAD+; 7.0 ± 0.7 fmol/mg tissue) is the primary nucleotide that contributes to the formation of eADPR. The tissue superfusates collected during EFS also contained the β-NAD+ metabolite ADPR (0.35 ± 0.2 fmol/mg tissue) but not cyclic ADPR (cADPR). HUB failed to degrade nicotinamide guanine dinucleotide (NGD+), a specific substrate of ADP ribosyl cyclase, suggesting that the activity of this enzyme in the HUB is negligible. The EFS-evoked release of β-NAD+ was frequency dependent and is reduced in the presence of tetrodotoxin (TTX; 0.3 μmol/l), ω-conotoxin GVIA (50 nmol/l), and botulinum neurotoxin A (BoNT/A; 100 nmol/l), but remained unchanged in the presence of guanethidine (3 μmol/l), ω-agatoxin IVA (50 nmol/l), or charbachol (1 μmol/l). Capsaicin (10 μmol/l) increased both the resting and EFS-evoked overflow of β-NAD+. Exogenous β-NAD+ (1 μmol/l) reduced both the frequency and amplitude of spontaneous contractions. In conclusion, we detected nerve-evoked overflow of β-NAD+ and ADPR in HUB. The β-NAD+/ADPR system may constitute a novel inhibitory extracellular nucleotide mechanism of neural control of the human bladder.

scholarly journals Mechanism of Action of Botulinum Toxin A in Treatment of Functional Urological Disorders

Toxins ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 129 ◽  
Author(s):  
Yu-Hua Lin ◽  
Bing-Juin Chiang ◽  
Chun-Hou Liao
Keyword(s):  
Botulinum Toxin ◽  
Overactive Bladder ◽  
Detrusor Overactivity ◽  
Botulinum Toxin A ◽  
Chronic Pelvic Pain Syndrome ◽  
Atp Release ◽  
Pain Syndrome ◽  
Pelvic Pain Syndrome ◽  
Detrusor Muscle ◽  
Urological Disorders

Intravesical botulinum toxin (BoNT) injection is effective in reducing urgency and urinary incontinence. It temporarily inhibits the detrusor muscle contraction by blocking the release of acetylcholine (Ach) from the preganglionic and postganglionic nerves in the efferent nerves. BoNT-A also blocks ATP release from purinergic efferent nerves in the detrusor muscle. In afferent nerves, BoNT-A injection markedly reduces the urothelial ATP release and increases nitric oxide (NO) release from the urothelium. BoNT-A injection in the urethra or bladder has been developed in the past few decades as the treatment method for detrusor sphincter dyssyndergia, incontinence due to neurogenic or idiopathic detrusor overactivity, sensory disorders, including bladder hypersensitivity, overactive bladder, and interstitial cystitis/chronic pelvic pain syndrome. Although the FDA only approved BoNT-A injection treatment for neurogenic detrusor overactivity and for refractory overactive bladder, emerging clinical trials have demonstrated the benefits of BoNT-A treatment in functional urological disorders. Cautious selection of patients and urodynamic evaluation for confirmation of diagnosis are crucial to maximize the successful outcomes of BoNT-A treatment.

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