scholarly journals Proteins and chemical chaperones involved in neuronal nicotinic receptor expression and function: an update

2017 ◽  
Vol 175 (11) ◽  
pp. 1869-1879 ◽  
Author(s):  
Arianna Crespi ◽  
Sara Francesca Colombo ◽  
Cecilia Gotti
Keyword(s):  
Nicotinic Receptor ◽  
Receptor Expression ◽  
Neuronal Nicotinic Receptor ◽  
Chemical Chaperones ◽  
And Function

scholarly journals Neonatal intermittent hypoxia impairs neuronal nicotinic receptor expression and function in adrenal chromaffin cells

2010 ◽  
Vol 299 (2) ◽  
pp. C381-C388 ◽  
Author(s):  
Dangjai Souvannakitti ◽  
Barbara Kuri ◽  
Guoxiang Yuan ◽  
Anita Pawar ◽  
Ganesh K. Kumar ◽  
...  
Keyword(s):  
Intermittent Hypoxia ◽  
Chromaffin Cells ◽  
Receptor Expression ◽  
Catecholamine Secretion ◽  
Pcr Analysis ◽  
Neuronal Nicotinic Receptor ◽  
Intracellular Ca ◽  
Adrenal Chromaffin ◽  
And Function ◽  
Reactive Oxygen Species Signaling

We recently reported that adrenomedullary chromaffin cells (AMC) from neonatal rats treated with intermittent hypoxia (IH) exhibit enhanced catecholamine secretion by hypoxia (Souvannakitti D, Kumar GK, Fox A, Prabhakar NR. J Neurophysiol 101: 2837–2846, 2009). In the present study, we examined whether neonatal IH also facilitate AMC responses to nicotine, a potent stimulus to chromaffin cells. Experiments were performed on rats exposed to either IH (15-s hypoxia-5-min normoxia; 8 h/day) or to room air (normoxia; controls) from ages postnatal day 0 (P0) to P5. Quantitative RT-PCR analysis revealed expression of mRNAs encoding α3-, α5-, α7-, and β2- and β4-nicotinic acetylcholine receptor (nAChR) subunits in adrenal medullae from control P5 rats. Nicotine-elevated intracellular Ca2+ concentration ([Ca2+]i) in AMC and nAChR antagonists prevented this response, suggesting that nAChRs are functional in neonatal AMC. In IH-treated rats, nAChR mRNAs were downregulated in AMC, which resulted in a markedly attenuated nicotine-evoked elevation in [Ca2+]i and subsequent catecholamine secretion. Systemic administration of antioxidant prevented IH-evoked downregulation of nAChR expression and function. P35 rats treated with neonatal IH exhibited reduced nAChR mRNA expression in adrenal medullae, attenuated AMC responses to nicotine, and impaired neurogenic catecholamine secretion. Thus the response to neonatal IH lasts for at least 30 days. These observations demonstrate that neonatal IH downregulates nAChR expression and function in AMC via reactive oxygen species signaling, and the effects of neonatal IH persist at least into juvenile life, leading to impaired neurogenic catecholamine secretion from AMC.

Role of the 807 C/T Polymorphism of the α2 Gene in Platelet GP Ia Collagen Receptor Expression and Function

1999 ◽  
Vol 81 (06) ◽  
pp. 951-956 ◽  
Author(s):  
J. Corral ◽  
R. González-Conejero ◽  
J. Rivera ◽  
F. Ortuño ◽  
P. Aparicio ◽  
...  
Keyword(s):  
Venous Thrombosis ◽  
Cell Types ◽  
Receptor Expression ◽  
Case Control Studies ◽  
Platelet Surface ◽  
Vitro Analysis ◽  
And Function ◽  
In Vitro Analysis

SummaryThe variability of the platelet GP Ia/IIa density has been associated with the 807 C/T polymorphism (Phe 224) of the GP Ia gene in American Caucasian population. We have investigated the genotype and allelic frequencies of this polymorphism in Spanish Caucasians. The T allele was found in 35% of the 284 blood donors analyzed. We confirmed in 159 healthy subjects a significant association between the 807 C/T polymorphism and the platelet GP Ia density. The T allele correlated with high number of GP Ia molecules on platelet surface. In addition, we observed a similar association of this polymorphism with the expression of this protein in other blood cell types. The platelet responsiveness to collagen was determined by “in vitro” analysis of the platelet activation and aggregation response. We found no significant differences in these functional platelet parameters according to the 807 C/T genotype. Finally, results from 3 case/control studies involving 302 consecutive patients (101 with coronary heart disease, 104 with cerebrovascular disease and 97 with deep venous thrombosis) determined that the 807 C/T polymorphism of the GP Ia gene does not represent a risk factor for arterial or venous thrombosis.

P2 receptor expression signaling and function in osteoclasts

10.2741/s214 ◽  
2011 ◽  
Vol S3 (3) ◽  
pp. 1101-1118
Author(s):  
S. Jeffrey Dixon
Keyword(s):  
Receptor Expression ◽  
P2 Receptor ◽  
And Function

Receptor Binding Thermodynamics at the Neuronal Nicotinic Receptor

2004 ◽  
Vol 4 (3) ◽  
pp. 361-368 ◽  
Author(s):  
P.A. Borea ◽  
K. Varani ◽  
S.G., S. Merighi ◽  
A.D. Piaz ◽  
P. Gilli ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Nicotinic Receptor ◽  
Neuronal Nicotinic Receptor ◽  
Binding Thermodynamics

scholarly journals Differential Regulation of Gonadotropins as Revealed by Transcriptomes of Distinct LH and FSH Cells of Fish Pituitary

2021 ◽  
Vol 22 (12) ◽  
pp. 6478
Author(s):  
Lian Hollander-Cohen ◽  
Matan Golan ◽  
Berta Levavi-Sivan
Keyword(s):  
Follicle Stimulating Hormone ◽  
Differential Regulation ◽  
Receptor Expression ◽  
Hormone Regulation ◽  
Fish Reproduction ◽  
Cell Type ◽  
Conserved Genes ◽  
Transgenic Tilapia ◽  
And Function ◽  
Direct Regulation

From mammals to fish, reproduction is driven by luteinizing hormone (LH) and follicle-stimulating hormone (FSH) temporally secreted from the pituitary gland. Teleost fish are an excellent model for addressing the unique regulation and function of each gonadotropin cell since, unlike mammals, they synthesize and secrete LH and FSH from distinct cells. Only very distant vertebrate classes (such as fish and birds) demonstrate the mono-hormonal strategy, suggesting a potential convergent evolution. Cell-specific transcriptome analysis of double-labeled transgenic tilapia expressing GFP and RFP in LH or FSH cells, respectively, yielded genes specifically enriched in each cell type, revealing differences in hormone regulation, receptor expression, cell signaling, and electrical properties. Each cell type expresses a unique GPCR signature that reveals the direct regulation of metabolic and homeostatic hormones. Comparing these novel transcriptomes to that of rat gonadotrophs revealed conserved genes that might specifically contribute to each gonadotropin activity in mammals, suggesting conserved mechanisms controlling the differential regulation of gonadotropins in vertebrates.

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