scholarly journals Reconstituting neurovascular unit with primary neural stem cells and brain microvascular endothelial cells in three‐dimensional matrix

2021 ◽  
Author(s):  
Hongjin Wang ◽  
Huan Yang ◽  
Yuhong Shi ◽  
Yaping Xiao ◽  
Yue Yin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Neural Stem Cells ◽  
Neurovascular Unit ◽  
Three Dimensional ◽  
Microvascular Endothelial Cells ◽  
Brain Microvascular Endothelial Cells ◽  
Dimensional Matrix ◽  
Microvascular Endothelial

scholarly journals Comparative study of expression and activity of glucose transporters between stem cell-derived brain microvascular endothelial cells and hCMEC/D3 cells

2017 ◽  
Vol 313 (4) ◽  
pp. C421-C429 ◽  
Author(s):  
Abraham J. Al-Ahmad
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Glucose Uptake ◽  
Glucose Transporter ◽  
Glucose Transporters ◽  
Microvascular Endothelial Cells ◽  
Brain Microvascular Endothelial Cells ◽  
Glucose Transporter 1 ◽  
Microvascular Endothelial

Glucose constitutes a major source of energy of mammalian brains. Glucose uptake at the blood-brain barrier (BBB) occurs through a facilitated glucose transport, through glucose transporter 1 (GLUT1), although other isoforms have been described at the BBB. Mutations in GLUT1 are associated with the GLUT1 deficiency syndrome, yet none of the current in vitro models of the human BBB maybe suited for modeling such a disorder. In this study, we investigated the expression of glucose transporters and glucose diffusion across brain microvascular endothelial cells (BMECs) derived from healthy patient-derived induced pluripotent stem cells (iPSCs). We investigated the expression of different glucose transporters at the BBB using immunocytochemistry and flow cytometry and measured glucose uptake and diffusion across BMEC monolayers obtained from two iPSC lines and from hCMEC/D3 cells. BMEC monolayers showed expression of several glucose transporters, in particular GLUT1, GLUT3, and GLUT4. Diffusion of glucose across the monolayers was mediated via a saturable transcellular mechanism and partially inhibited by pharmacological inhibitors. Taken together, our study suggests the presence of several glucose transporters isoforms at the human BBB and demonstrates the feasibility of modeling glucose across the BBB using patient-derived stem cells.

scholarly journals Proliferation and neurogenesis of neural stem cells enhanced by cerebral microvascular endothelial cells

Microsurgery ◽  
2007 ◽  
Vol 28 (1) ◽  
pp. 54-60 ◽  
Author(s):  
Ying Guo ◽  
Dejin Shi ◽  
Wensheng Li ◽  
Chaofeng Liang ◽  
Hui Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Neural Stem Cells ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial
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