scholarly journals Spinal Lewy body pathology in older adults without an antemortem diagnosis of Parkinson's disease

2017 ◽  
Vol 28 (4) ◽  
pp. 560-568 ◽  
Author(s):  
Aron S. Buchman ◽  
Sukriti Nag ◽  
Sue E. Leurgans ◽  
Jared Miller ◽  
Veronique G. J. M. VanderHorst ◽  
...  
Keyword(s):  
Older Adults ◽  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Lewy Body ◽  
Lewy Body Pathology

scholarly journals A Matrigel-based 3D construct of SH-SY5Y cells models the α-synuclein pathologies of Parkinson's disease

2022 ◽  
Author(s):  
Zhao-Feng Li ◽  
Lei Cui ◽  
Mi-Mi Jin ◽  
Dong-Yan Hu ◽  
Xiao-Gang Hou ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Lewy Body ◽  
Cultured Cells ◽  
Three Dimensional ◽  
Proteinase K ◽  
All Trans Retinoic Acid ◽  
Lewy Body Pathology ◽  
Rotenone Treatment ◽  
2D And 3D

Parkinson's disease (PD) is featured with α-synuclein-based Lewy body pathology, which however was difficult to observe in conventional two-dimensional (2D) cell culture and even in animal models. We herein aimed to develop a three-dimensional (3D) cellular model of PD to recapitulate the α-synuclein pathologies. All-trans-retinoic acid-differentiated human SH-SY5Y cells and Matrigel were optimized for 3D construction. The 3D cultured cells displayed higher tyrosine hydroxylase expression and improved dopaminergic-like phenotypes than 2D cells as suggested by RNA-sequencing analyses. Multiple forms of α-synuclein, including monomer, low and high molecular weight oligomers, were differentially present in the 2D and 3D cells, but mostly remained unchanged upon the MPP+ or rotenone treatment. Phosphorylated α-synuclein was accumulated and detergent-insoluble α-synuclein fraction was observed in the neurotoxin-treated 3D cells. Importantly, Lewy body-like inclusions were captured in the 3D system, including proteinase K-resistant α-synuclein aggregates, ubiquitin aggregation, β-amyloid and β-sheet protein deposition. The study provides a unique and convenient 3D model of PD which recapitulates critical α-synuclein pathologies and should be useful in multiple PD-associated applications.

scholarly journals Parkinson's disease susceptibility variants and severity of Lewy body pathology

2017 ◽  
Vol 44 ◽  
pp. 79-84 ◽  
Author(s):  
Michael G. Heckman ◽  
Koji Kasanuki ◽  
Nancy N. Diehl ◽  
Shunsuke Koga ◽  
Alexandra Soto ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Lewy Body ◽  
Disease Susceptibility ◽  
Lewy Body Pathology

Lewy body pathology involves the olfactory cells in Parkinson's disease and related disorders

2016 ◽  
Vol 31 (1) ◽  
pp. 135-138 ◽  
Author(s):  
Yuko Saito ◽  
Ayako Shioya ◽  
Terunori Sano ◽  
Hiroyuki Sumikura ◽  
Miho Murata ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Lewy Body ◽  
Lewy Body Pathology ◽  
Olfactory Cells

scholarly journals Divergent α-synuclein solubility and aggregation properties in G2019S LRRK2 Parkinson's disease brains with Lewy Body pathology compared to idiopathic cases

2013 ◽  
Vol 58 ◽  
pp. 183-190 ◽  
Author(s):  
Adamantios Mamais ◽  
Meera Raja ◽  
Claudia Manzoni ◽  
Sybille Dihanich ◽  
Andrew Lees ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Lewy Body ◽  
Lewy Body Pathology

scholarly journals LRRK2-Related Parkinson’s Disease Due to Altered Endolysosomal Biology With Variable Lewy Body Pathology: A Hypothesis

2020 ◽  
Vol 14 ◽  
Author(s):  
Pilar Rivero-Ríos ◽  
María Romo-Lozano ◽  
Rachel Fasiczka ◽  
Yahaira Naaldijk ◽  
Sabine Hilfiker
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Lewy Body ◽  
Lewy Body Pathology
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