scholarly journals Altered Loyalties of Neuronal Markers in Cultured Slices of Resected Human Brain Tissue

2015 ◽  
Vol 26 (4) ◽  
pp. 523-532 ◽  
Author(s):  
Ronald W. H. Verwer ◽  
Arja A. Sluiter ◽  
Rawien A. Balesar ◽  
Johannes C. Baayen ◽  
Dave Speijer ◽  
...  
Keyword(s):  
Human Brain ◽  
Brain Tissue ◽  
Human Brain Tissue ◽  
Neuronal Markers ◽  
Cultured Slices

GFAP-positive astrocytes are rare or absent in primary adult human brain tissue cultures

Biologia ◽  
2009 ◽  
Vol 64 (4) ◽  
Author(s):  
Ivana Macikova ◽  
Anna Perzelova ◽  
Peter Mraz ◽  
Ivan Bizik ◽  
Juraj Steno
Keyword(s):  
White Matter ◽  
Human Brain ◽  
Brain Tissue ◽  
Cell Phenotype ◽  
Human Brain Tissue ◽  
Adult Human Brain ◽  
Neuronal Markers ◽  
Adult Human

AbstractTraditionally, astrocytes are divided into fibrous and protoplasmic types based on their morphologic appearance. Here the cultures were prepared separately from the adult human cortical gray and white matter of brain biopsies. Both cultures differed only in the number of glial fibrillary acidic protein (GFAP)-positive cells. In the gray matter these were absent or rare, whereas in confluent cultures from the white matter they reached 0.1% of all cells. Three main morphologic types of GFAP-positive cells were found in this study: stellate, bipolar and large flat cells. GFAP-positive cells with two or three long processes mimic a neuron-like morphology. We did not find process-bearing cells expressing neuronal markers (MAP-2, NF, and N-CAM). The conflicting reports concerning GFAP immunostaining and the study dealing with the presence of putative neurons in adult human brain cultures are discussed with respect to these findings. The latter classification of astrocytes into type 1 and type 2 is based on immunostaining to A2B5 antigen: type 1 (GFAP+/A2B5−) and type 2 (GFAP+/A2B5+) astrocytes are proposed to be analogous to protoplasmic and fibrous astrocytes, respectively. In adult human brain cultures we found only small amount of A2B5-positive cells. Double immunofluorescence revealed that astroglial cells of similar fibrous or bipolar shape grown on one coverslip were either GFAP+/A2B5+ or GFAP+/A2B5−. On the other hand, the A2B5+/GFAP− immunophenotype was not observed. These results indicate that in general the cell phenotype from adult human brain tissue is not well established when they are in culture.

scholarly journals N-Acetylaspartate and 2-Hydroxyglutarate Assessed in Human Brain Tissue by Mass Spectrometry as Neuronal Markers of Oncogenesis

2017 ◽  
Vol 63 (11) ◽  
pp. 1766-1767 ◽  
Author(s):  
Karen E Yannell ◽  
Katelynn Smith ◽  
Clint M Alfaro ◽  
Alan K Jarmusch ◽  
Valentina Pirro ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Human Brain ◽  
Brain Tissue ◽  
Human Brain Tissue ◽  
Neuronal Markers

scholarly journals RBIO-06. IMPLEMENTATION OF HUMAN INDUCED PLURIPOTENT STEM CELL DERIVED CEREBRAL ORGANOIDS TO MODEL NORMAL TISSUE RADIORESPONSE

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii193-ii193
Author(s):  
Lawrence Bronk ◽  
Sanjay Singh ◽  
Riya Thomas ◽  
Luke Parkitny ◽  
Mirjana Maletic-Savatic ◽  
...  
Keyword(s):  
Stem Cell ◽  
Human Brain ◽  
Brain Tissue ◽  
Human Tissue ◽  
Model Systems ◽  
Human Brain Tissue ◽  
Post Irradiation ◽  
Mature Neurons ◽  
Induced Pluripotent ◽  
Effects Of Radiation

Abstract Treatment-related sequelae following cranial irradiation have life changing impacts for patients and their caregivers. Characterization of the basic response of human brain tissue to irradiation has been difficult due to a lack of preclinical models. The direct study of human brain tissue in vitro is becoming possible due to advances in stem cell biology, neuroscience, and tissue engineering with the development of organoids as novel model systems which enable experimentation with human tissue models. We sought to establish a cerebral organoid (CO) model to study the radioresponse of normal human brain tissue. COs were grown using human induced pluripotent stem cells and a modified Lancaster protocol. Compositional analysis during development of the COs showed expected populations of neurons and glia. We confirmed a population of microglia-like cells within the model positive for the makers Iba1 and CD68. After 2-months of maturation, COs were irradiated to 0, 10, and 20 Gy using a Shepard Mark-II Cs-137 irradiator and returned to culture. Subsets of COs were prepared for immunostaining at 30- and 70-days post-irradiation. To examine the effect of irradiation on the neural stem cell (NSC) population, sections were stained for SOX2 and Ki-67 expression denoting NSCs and proliferation respectively. Slides were imaged and scored using the CellProfiler software package. The percentage of proliferating NSCs 30-days post-irradiation was found to be significantly reduced for irradiated COs (5.7% (P=0.007) and 3.4% (P=0.001) for 10 and 20 Gy respectively) compared to control (12.7%). The reduction in the proliferating NSC population subsequently translated to a reduced population of NeuN-labeled mature neurons 70 days post-irradiation. The loss of proliferating NSCs and subsequent reduction in mature neurons demonstrates the long-term effects of radiation. Our initial results indicate COs will be a valuable model to study the effects of radiation therapy on normal and diseased human tissue.

Demonstration of α1A-and α1B-adrenoceptor binding sites in human brain tissue

1989 ◽  
Vol 169 (2-3) ◽  
pp. 325-328 ◽  
Author(s):  
Gerhard Gross ◽  
Gertraud Hanft ◽  
Hubertus M. Mehdorn
Keyword(s):  
Human Brain ◽  
Brain Tissue ◽  
Binding Sites ◽  
Human Brain Tissue

The lipopigments in human brain tissue necroses

1980 ◽  
Vol 52 (2) ◽  
pp. 147-151 ◽  
Author(s):  
R. Schr�der ◽  
B. Reinartz
Keyword(s):  
Human Brain ◽  
Brain Tissue ◽  
Human Brain Tissue
Sign in / Sign up

Export Citation Format

Share Document