scholarly journals Differential expression of long non-coding RNAs are related to proliferation and histological diversity in follicular lymphomas

2018 ◽  
Vol 184 (3) ◽  
pp. 373-383 ◽  
Author(s):  
Alejandro Roisman ◽  
Giancarlo Castellano ◽  
Alba Navarro ◽  
Blanca Gonzalez-Farre ◽  
Patricia Pérez-Galan ◽  
...  
Keyword(s):  
Differential Expression ◽  
Non Coding Rnas ◽  
Follicular Lymphomas

scholarly journals lncRNAs in development and disease: from functions to mechanisms

Open Biology ◽  
2017 ◽  
Vol 7 (7) ◽  
pp. 170121 ◽  
Author(s):  
M. Joaquina Delás ◽  
Gregory J. Hannon
Keyword(s):  
Differential Expression ◽  
Cell Fate ◽  
Mode Of Action ◽  
Non Coding Rnas

Differential expression of long non-coding RNAs (lncRNAs) during differentiation and their misregulation in cancer highlight their potential as cell fate regulators. While some example lncRNAs have been characterized in great detail, the functional in vivo relevance of others has been called into question. Finding functional lncRNAs will most probably require a combination of complementary approaches that will greatly vary depending on their mode of action. In this review, we discuss the different tools available to dissect genetically lncRNA requirements and how each is best suited to studies in particular contexts. Moreover, we review different strategies used to select candidate lncRNAs and give an overview of lncRNAs described to regulate development and cancer through different mechanisms.

Differential expression of H19, BC1, MIAT1, and MALAT1 long non-coding RNAs within key brain reward regions after repeated morphine treatment

2021 ◽  
pp. 113478
Author(s):  
Shamseddin Ahmadi ◽  
Mohammad Zobeiri ◽  
Shiva Mohammadi Talvar ◽  
Kayvan Masoudi ◽  
Amir Khanizad ◽  
...  
Keyword(s):  
Differential Expression ◽  
Morphine Treatment ◽  
Non Coding Rnas ◽  
Brain Reward

scholarly journals Exosomal microRNAs are novel circulating biomarkers in cigarette, waterpipe smokers, E-cigarette users and dual smokers

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Kameshwar P. Singh ◽  
Krishna P. Maremanda ◽  
Dongmei Li ◽  
Irfan Rahman
Keyword(s):  
Differential Expression ◽  
Tobacco Smoking ◽  
Dna Analysis ◽  
Electronic Cigarettes ◽  
Differential Expression Analysis ◽  
High Sensitivity ◽  
Gene Set Enrichment Analysis ◽  
Differentially Expressed ◽  
Cigarette Smokers ◽  
Non Coding Rnas

Abstract Background Electronic cigarettes (e-cigs) vaping, cigarette smoke, and waterpipe tobacco smoking are associated with various cardiopulmonary diseases. microRNAs are present in higher concentration in exosomes that play an important role in various physiological and pathological functions. We hypothesized that the non-coding RNAs transcript may serve as susceptibility to disease biomarkers by smoking and vaping. Methods Plasma exosomes/EVs from cigarette smokers, waterpipe smokers and dual smokers (cigarette and waterpipe) were characterized for their size, morphology and TEM, Nanosight and immunoblot analysis. Exosomal RNA was used for small RNA library preparation and the library was quantified using the High Sensitivity DNA Analysis on the Agilent 2100 Bioanalyzer system and sequenced using the Illumina NextSeq 500 and were converted to fastq format for mapping genes. Results Enrichment of various non-coding RNAs that include microRNAs, tRNAs, piRNAs, snoRNAs, snRNAs, Mt-tRNAs, and other biotypes are shown in exosomes. A comprehensive differential expression analysis of miRNAs, tRNAs and piRNAs showed significant changes across different pairwise comparisons. The seven microRNAs that were common and differentially expressed of when all the smoking and vaping groups were compared with non-smokers (NS) are hsa-let-7a-5p, hsa-miR-21-5p, hsa-miR-29b-3p, hsa-let-7f-5p, hsa-miR-143-3p, hsa-miR-30a-5p and hsa-let-7i-5p. The e-cig vs. NS group has differentially expressed 5 microRNAs (hsa-miR-224-5p, hsa-miR-193b-3p, hsa-miR-30e-5p, hsa-miR-423-3p, hsa-miR-365a-3p, and hsa-miR-365b-3p), which are not expressed in other three groups. Gene set enrichment analysis of microRNAs showed significant changes in the top six enriched functions that consisted of biological pathway, biological process, molecular function, cellular component, site of expression and transcription factor in all the groups. Further, the pairwise comparison of tRNAs and piRNA in all these groups revealed significant changes in their expressions. Conclusions Plasma exosomes of cigarette smokers, waterpipe smokers, e-cig users and dual smokers have common differential expression of microRNAs which may serve to distinguish smoking and vaping subjects from NS. Among them has-let-7a-5p has high sensitivity and specificity to distinguish NS with the rest of the users, using ROC curve analysis. These findings will pave the way for the utilizing the potential of exosomes/miRNAs as a novel theranostic agents in lung injury and disease caused by tobacco smoking and vaping.

scholarly journals Differential expression of circulating long non-coding RNAs in patients with acute myocardial infarction

Medicine ◽  
2018 ◽  
Vol 97 (51) ◽  
pp. e13066 ◽  
Author(s):  
Zhixiong Zhong ◽  
Jingyuan Hou ◽  
Qifeng Zhang ◽  
Bin Li ◽  
Cunren Li ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Differential Expression ◽  
Non Coding Rnas

Differential expression profile of long non-coding RNAs in human thoracic aortic aneurysm

2018 ◽  
Vol 119 (10) ◽  
pp. 7991-7997 ◽  
Author(s):  
Yang Li ◽  
Yang Liu ◽  
Suxuan Liu ◽  
Feng Wu ◽  
Songhua Li ◽  
...  
Keyword(s):  
Aortic Aneurysm ◽  
Differential Expression ◽  
Expression Profile ◽  
Thoracic Aortic Aneurysm ◽  
Differential Expression Profile ◽  
Non Coding Rnas

Differential expression of microRNAs in porcine placentas on Days 30 and 90 of gestation

2010 ◽  
Vol 22 (8) ◽  
pp. 1175 ◽  
Author(s):  
Lijie Su ◽  
Shuhong Zhao ◽  
Mengjin Zhu ◽  
Mei Yu
Keyword(s):  
Differential Expression ◽  
Differentially Expressed ◽  
Locked Nucleic Acid ◽  
Chain Reaction ◽  
Stem Loop ◽  
Non Invasive ◽  
Differentially Expressed Mirnas ◽  
Non Coding Rnas ◽  
Polymerase Chain ◽  
And Function

The porcine placenta is classified as a non-invasive epitheliochorial type. To meet the increasing demands for nutrients by the rapidly growing conceptus and/or fetus, the placental microscopic folds undergo significant morphological and biochemical changes during two periods critical for conceptus and/or fetus, namely Days 30–40 and after Day 90 of gestation. MicroRNAs (miRNAs) are a class of small non-coding RNAs that can modulate gene activity by inhibiting the translation or regulation of mRNA degradation. In the present study, we identified 17 differentially expressed miRNAs in porcine placenta on Days 30 and 90 of gestation using a locked nucleic acid (LNA) microRNA array. Stem–loop real-time reverse transcription–polymerase chain reaction confirmed the differential expression of eight selected miRNAs (miR-24, miR-125b, miR-92b, miR-106a, miR-17, let-7i, miR-27a and miR-20). Analysis of targets and the pathways in which these miRNAs are involved revealed that the differentially expressed miRNAs target many genes that are important in various processes, including cell growth, trophoblast differentiation, angiogenesis and formation and maintenance of adherens junctions. The results of the present study suggest potential roles for these differentially expressed miRNAs in porcine placental growth and function.

Long non‐coding RNAs differential expression in breast cancer subtypes: What do we know?

2019 ◽  
Vol 95 (5) ◽  
pp. 558-568 ◽  
Author(s):  
Carolina Mathias ◽  
Erika P. Zambalde ◽  
Philip Rask ◽  
Daniela F. Gradia ◽  
Jaqueline C. de Oliveira
Keyword(s):  
Breast Cancer ◽  
Differential Expression ◽  
Breast Cancer Subtypes ◽  
Cancer Subtypes ◽  
Non Coding Rnas
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