Auer rods in neutrophils in bone marrow and peripheral blood in mixed phenotype acute leukaemia in a child

2018 ◽  
Vol 184 (5) ◽  
pp. 708-708
Author(s):  
Samia Kabbage ◽  
Roberto Cupaiolo ◽  
Laurence Rozen ◽  
Anne Demulder
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Acute Leukaemia ◽  
Mixed Phenotype

scholarly journals Acute leukemia in sickle cell disease patients in a tertiary health facility in Nigeria: a case series

2020 ◽  
Vol 20 (3) ◽  
pp. 1304-1312 ◽  
Author(s):  
Oladapo W Aworanti ◽  
Foluke A Fasola ◽  
Taiwo R Kotila ◽  
John A Olaniyi ◽  
Biobele J Brown
Keyword(s):  
Bone Marrow ◽  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Peripheral Blood ◽  
Acute Leukaemia ◽  
Film Review ◽  
Case Series ◽  
Blood Film ◽  
Cell Disease ◽  
Peripheral Blood Film

Background and objectives: Sickle cell disease(SCD) is a disorder of red cells resulting from the co-inheritance of hae- moglobin S (HbS) with another abnormal haemoglobin. The diagnosis of acute leukaemia is uncommon in our patients with sickle cell disease more so the patients have high morbidity and mortality due to the sickling process.Acute leukemia is a malignant clonal disorder of haemopoietic precursor cells resulting in accumulation of immature blood cells in the bone marrow and blood.The objective of the case series was to highlight the challenges of diagnosis and management of SCD patients with acute leukaemia, the importance of peripheral blood film review and propound a possible risk factor. Methods: Records of 58 patients diagnosed and managed for acute leukaemia over a 7 year period at the University College Hospital, Ibadan were reviewed. The diagnosis of acute leukaemia was based on clinical features in addition to peripheral and bone marrow smears findings. Microsoft excel version 2013 was used for statistical analysis. Results: Five (8.6%) of the patients with acute leukaemia also had sickle cell disease: 3 males and 2 females were described. Recurrent fever and anaemia were the most consistent presenting features in the patients. All the patients were not on any routine medications meant for SCD patients and had poor history of clinic attendance prior to the diagnosis of acute leu- kaemia. The diagnosis of acute leukaemia was not made until the patients were seen by a haematologist. The principal tool of diagnosis in all the patients was peripheral blood film review. Two patients were discharged against medical advice.The treatment period ranged between one month and one year in the remaining three patients. Conclusion: SCD patients are not exempted from developing acute leukaemias and the diagnoses of the two conditions overwhelms the social and economic support of patients and care givers.The study also underscores the relevance of high level of suspicion and prompt review of peripheral blood film of SCD patients particularly when patients present with un- remitting symptoms associated with anaemia and fever. Keywords: Acute leukaemia; sickle cell disease; anaemia.

scholarly journals [Cytological features of acute leukaemia in the central region of Tunisia]

2004 ◽  
Vol 10 (4-5) ◽  
pp. 640-647
Author(s):  
N. Braham Jmili ◽  
A. Ben Abdel Aziz ◽  
M. Nagara ◽  
T. Mahjoub ◽  
H. Ghannem ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Acute Lymphoblastic Leukaemia ◽  
Central Region ◽  
Lymphoblastic Leukaemia ◽  
Peripheral Blood ◽  
Acute Leukaemia ◽  
Acute Myeloblastic Leukemia ◽  
Registry Data ◽  
Cytological Features ◽  
Age Range

In Tunisia, because of an absence of population registry, data on acute leukaemia are scarce. We studied the epidemiological and cytological characteristic of 193 patients with acute leukaemia. Haemograms were carried out and slides for peripheral blood and bone marrow were prepared for each patient. The age range of the patients was 10 months to 83 years with a predominance of males [ratio:1.27]. As regards type of leukaemia, 40.4% had acute lymphoblastic leukaemia, 51.8% had acute myeloblastic leukemia and 7.8% were unclassified. Diagnosis was made at less than 10 years in 31.6% of cases and 72% of these were the lymphoblastic type. Anaemia [Hb < 11 g/dL was found in 85% of cases, thrombocytopenia [platelets < 100 000/mm3] in 80.5% and hyperleukocytosis [WBC > 100 000/mm3] in 14.5% of cases with blasts in peripheral blood in 92% of cases

Neutrophil pseudoplatelets in subgingival plaque and crevicular fluid samples from periodontal diseased sites

1989 ◽  
Vol 47 ◽  
pp. 862-863 ◽  
Author(s):  
J Hanker ◽  
E.J. Burkes ◽  
G. Greco ◽  
R. Scruggs ◽  
B. Giammara
Keyword(s):  
Electron Microscopy ◽  
Bone Marrow ◽  
Peripheral Blood ◽  
Gingival Crevicular Fluid ◽  
Light And Electron Microscopy ◽  
Subgingival Plaque ◽  
Staining Reaction ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Crevicular Fluid

The mature neutrophil with a segmented nucleus (usually having 3 or 4 lobes) is generally considered to be the end-stage cell of the neutrophil series. It is usually found as such in the bone marrow and peripheral blood where it normally is the most abundant leukocyte. Neutrophils, however, must frequently leave the peripheral blood and migrate into areas of infection to combat microorganisms. It is in such areas that neutrophils were first observed to fragment to form platelet-size particles some of which have a nuclear lobe. These neutrophil pseudoplatelets (NPP) can readily be distinguished from true platelets because they stain for neutrophil myeloperoxidase. True platelets are not positive in this staining reaction because their peroxidase Is inhibited by glutaraldehyde. Neutrophil pseudoplatelets, as well as neutrophils budding to form NPP, could frequently be observed in peripheral blood or bone marrow samples of leukemia patients. They are much more prominent, however, in smears of inflammatory exudates that contain gram-negative bacteria and in gingival crevicular fluid samples from periodontal disease sites. In some of these samples macrophages ingesting, or which contained, pseudoplatelets could be observed. The myeloperoxidase in the ingested pseudoplatelets was frequently active. Despite these earlier observations we did not expect to find many NPP in subgingival plaque smears from diseased sites. They were first seen by light microscopy (Figs. 1, 3-5) in smears on coverslips stained with the PATS reaction, a variation of the PAS reaction which deposits silver for light and electron microscopy. After drying replicate PATS-stained coverslips with hexamethyldisilazane, they were sputter coated with gold and then examined by the SEI and BEI modes of scanning electron microscopy (Fig. 2). Unstained replicate coverslips were fixed, and stained for the demonstration of myeloperoxidase in budding neutrophils and NPP. Neutrophils, activated macrophages and spirochetes as well as other gram-negative bacteria were also prominent in the PATS stained samples. In replicate subgingival plaque smears stained with our procedure for granulocyte peroxidases only neutrophils, budding neutrophils or NPP were readily observed (Fig. 6).

The human bone marrow (BM) niche in acute leukaemia

2017 ◽  
Author(s):  
D Pal ◽  
H Blair ◽  
S Boyd ◽  
P Bakelis ◽  
A Elder ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Acute Leukaemia ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bm Niche
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