scholarly journals Temporal definition of haematopoietic stem cell niches in a large animal model ofin uterostem cell transplantation

2014 ◽  
Vol 166 (2) ◽  
pp. 268-278 ◽  
Author(s):  
Christine Jeanblanc ◽  
Angelina Daisy Goodrich ◽  
Evan Colletti ◽  
Saloomeh Mokhtari ◽  
Christopher D. Porada ◽  
...  
Keyword(s):  
Animal Model ◽  
Stem Cell ◽  
Cell Transplantation ◽  
Haematopoietic Stem Cell ◽  
Large Animal Model ◽  
Large Animal ◽  
Definition Of ◽  
Stem Cell Niches

Posttransplantation lymphoproliferative disease in miniature swine after allogeneic hematopoietic cell transplantation: similarity to human PTLD and association with a porcine gammaherpesvirus

Blood ◽  
2001 ◽  
Vol 97 (5) ◽  
pp. 1467-1473 ◽  
Author(s):  
Christene A. Huang ◽  
Yasushi Fuchimoto ◽  
Zachary L. Gleit ◽  
Thomas Ericsson ◽  
Adam Griesemer ◽  
...  
Keyword(s):  
Animal Model ◽  
Stem Cell ◽  
Stem Cell Transplantation ◽  
Cell Transplantation ◽  
Peripheral Blood ◽  
Lymphoproliferative Disease ◽  
Large Animal Model ◽  
Large Animal ◽  
Miniature Swine ◽  
Hematopoietic Stem

Posttransplantation lymphoproliferative disease (PTLD) is a major complication of current clinical transplantation regimens. The lack of a reproducible large-animal model of PTLD has limited progress in understanding the pathogenesis of and in developing therapy for this clinically important disease. This study found a high incidence of PTLD in miniature swine undergoing allogeneic hematopoietic stem cell transplantation and characterized this disease in swine. Two days before allogeneic peripheral blood stem cell transplantation, miniature swine were conditioned with thymic irradiation and in vivo T-cell depletion. Animals received cyclosporine daily beginning 1 day before transplantation and continuing for 30 to 60 days. Flow cytometry and histologic examination were performed to determine the cell type involved in lymphoproliferation. Polymerase chain reaction was developed to detect and determine the level of porcine gammaherpesvirus in involved lymph node tissue. PTLD in swine is morphologically and histologically similar to that observed in human allograft recipients. Nine of 21 animals developed a B-cell lymphoproliferation involving peripheral blood (9 of 9), tonsils, and lymph nodes (7 of 9) from 21 to 48 days after transplantation. Six of 9 animals died of PTLD and 3 of 9 recovered after reduction of immunosuppression. A novel porcine gammaherpesvirus was identified in involved tissues. Miniature swine provide a genetically defined large-animal model of PTLD with many characteristics similar to human PTLD. The availability of this reproducible large-animal model of PTLD may facilitate the development and testing of diagnostic and therapeutic approaches for prevention or treatment of PTLD in the clinical setting.

scholarly journals Large Animal Model Efficacy Testing Is Needed Prior to Launch of a Stem Cell Clinical Trial

2017 ◽  
Vol 121 (5) ◽  
pp. 496-498 ◽  
Author(s):  
Stephen E. Epstein ◽  
Dror Luger ◽  
Michael J. Lipinski
Keyword(s):  
Clinical Trial ◽  
Animal Model ◽  
Stem Cell ◽  
Large Animal Model ◽  
Large Animal

PL12. Tissue-Engineered Small Intestine and Stomach Demonstrate Features of an Intact Stem Cell Niche in a Large Animal Model

2009 ◽  
Vol 151 (2) ◽  
pp. 279-280
Author(s):  
F.G. Sala ◽  
S. Kunisaki ◽  
E. Ochoa ◽  
J.P. Vacanti ◽  
T.C. Grikscheit
Keyword(s):  
Animal Model ◽  
Small Intestine ◽  
Stem Cell ◽  
Stem Cell Niche ◽  
Large Animal Model ◽  
Large Animal ◽  
Cell Niche

scholarly journals Refractory autoimmune haemolytic anaemia following allogenic haematopoietic stem cell transplantation: successful treatment of rituximab

2019 ◽  
Vol 47 (7) ◽  
pp. 3320-3331
Author(s):  
Xiaofan Li ◽  
Jiafu Huang ◽  
Zhijuan Zhu ◽  
Nainong Li
Keyword(s):  
Animal Model ◽  
Stem Cell ◽  
Stem Cell Transplantation ◽  
Cell Transplantation ◽  
Haemolytic Anaemia ◽  
Haematopoietic Stem Cell Transplantation ◽  
Chronic Gvhd ◽  
Autoimmune Haemolytic Anaemia ◽  
Haematopoietic Stem Cell ◽  
Anti Cd20

Objective To investigate the effectiveness and safety of rituximab in treating autoimmune haemolytic anaemia (AIHA) after allogeneic haematopoietic stem cell transplantation (allo-HSCT). Methods Patients with refractory AIHA following allo-HSCT were treated once-weekly with rituximab 375 mg/m2 for a total of four doses. In an animal study, recipient CB6F1 mice were conditioned with busulfan/fludarabine and transplanted with splenocytes and T-cell-depleted bone marrow from C57Bl/6 mice. In this animal model, anti-CD20 monoclonal antibody (mAb) was evaluated to see if it could prevent graft versus host disease (GVHD). GVHD was monitored by body weight loss, GVHD clinical scores and the survival of each group of mice. Histopathological analyses of the skin, intestine, liver and lung were used to analyse the severity of GVHD. Results After rituximab therapy, refractory AIHA was resolved in all four patients as shown by increased haemoglobin levels. B-cell proportions were reduced with a relative increase of the proportions of T-cells following rituximab treatment. None of the four patients experienced chronic GVHD. In the animal model, anti-CD20 mAb treatment reduced GVHD. Conclusions Rituximab therapy deserves consideration for the treatment of post-HSCT patients with refractory AIHA. Further studies are needed to define the therapeutic role of this anti-CD20 mAb.

Mesenchymal stem cell derived exosomes in the treatment of synovitis: proof of concept in large animal model

2016 ◽  
Vol 33 (3) ◽  
pp. 409
Author(s):  
F. Javier Vela González ◽  
Francisco M. Sánchez-Margallo ◽  
Angelo Tapia-Araya ◽  
Rebeca Blázquez ◽  
Verónica Álvarez ◽  
...  
Keyword(s):  
Animal Model ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Large Animal Model ◽  
Large Animal ◽  
Proof Of Concept
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