Persistent Lyme disease with cutaneous borrelia biofilm formation

British Journal of Dermatology ◽

10.1111/bjd.20977 ◽

2022 ◽

Author(s):

Anna Gindl ◽

Anna‐Margarita Schötta ◽

Sara Berent ◽

Mateusz Markowicz ◽

Hannes Stockinger ◽

...

Keyword(s):

Lyme Disease ◽

Biofilm Formation

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Bacterial biofilm behavior in water-supply lines of dental units

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100124811 ◽

1992 ◽

Vol 50 (1) ◽

pp. 882-883

Author(s):

B.D. Tall ◽

K.S. George ◽

R. T. Gray ◽

H.N. Williams

Keyword(s):

Water Supply ◽

Medical Devices ◽

Biofilm Formation ◽

Bacterial Biofilm

Studies of bacterial behavior in many environments have shown that most organisms attach to surfaces, forming communities of microcolonies called biofilms. In contaminated medical devices, biofilms may serve both as reservoirs and as inocula for the initiation of infections. Recently, there has been much concern about the potential of dental units to transmit infections. Because the mechanisms of biofilm formation are ill-defined, we investigated the behavior and formation of a biofilm associated with tubing leading to the water syringe of a dental unit over a period of 1 month.

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New ultrastructural findings about Borrelia burgdorferi by cryofixation, negative staining, thin sectioning and scanning techniques

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100160467 ◽

1990 ◽

Vol 48 (3) ◽

pp. 582-583

Author(s):

S. F. Hayes ◽

M. D. Corwin ◽

T. G. Schwan ◽

D. W. Dorward ◽

W. Burgdorfer

Keyword(s):

Lyme Disease ◽

Borrelia Burgdorferi ◽

Structural Characterization ◽

Negative Staining ◽

Low Speed ◽

Thin Sectioning ◽

Ultrastructural Findings

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.

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