scholarly journals H01: The haematoxylin and eosin stain: from piracy to pathology

2021 ◽  
Vol 185 (S1) ◽  
pp. 161-161
Keyword(s):  
Haematoxylin And Eosin Stain

DIAGNOSTIC ACCURACY OF SQUASH CYTOLOGY OF ASTROCYTOMA

2021 ◽  
pp. 57-58
Author(s):  
M. S. Lalitha ◽  
A. G. Krishnaveni ◽  
A. Vijayalakshmi
Keyword(s):  
Central Nervous System ◽  
Cost Effective ◽  
Rapid Diagnosis ◽  
Accurate Diagnosis ◽  
Intracranial Tumour ◽  
Diffuse Astrocytoma ◽  
Squash Preparation ◽  
Central Nervous System Tumours ◽  
Extent Of Surgery ◽  
Haematoxylin And Eosin Stain

BACKGROUND: Primary Central nervous system tumours occupies less than 2% of overall human cancers in adults. The accurate diagnosis of intracranial tumour is necessary for therapeutic and prognostic purpose. Intraoperative smear cytology provides a rapid diagnosis which helps the neurosurgeon for immediate decision regarding the extent of surgery. To Objectives: determine the accuracy of squash preparation, by comparing it with histopathological sections and analysing the cytomorphological features of astrocytoma This was both retrospective and prospective study. We receive Methods: d nineteen radiologically and clinically suspected Astrocytoma in saline with xatives. Crush smear were made and stained with rapid Haematoxylin and Eosin. The corresponding biopsy materials were xed in 10% neutral buffered formalin and submitted for tissue processing. Staining was done with routine Haematoxylin and Eosin stain. The cytomorphologicalfeatures of these tumours were correlated with histopathological sections. In our study, we received Results: nineteen radiologically and clinically suspected Astrocytoma , tissue of all the tumours were soft and easy to smear . Glioblastomamultiforme was the most commonly encounterd tumor amomgneuroepithelial tumor constituting about 42.1% followed by diffuse astrocytoma (36.8%), WHOgrade II and IV tumors of astrocytoma were commonly encountered in ourstudy. Astrocytoma was common in males than females with majority of them fall in 5 th decade. we got 94.7% correlation between squash cytology and histopathology. Squash cytology is Conclusion: simple,rapid, accurate and cost effective diagnostic tool for Astrocytoma

scholarly journals Ovarian Cancer: Diagnostic Accuracy and Tumor Types Distribution in East Africa compared to North America

2020 ◽  
Author(s):  
Peter Fabian Rambau ◽  
Martin Köbel ◽  
Derek Tilley ◽  
Alex Mremi ◽  
Robert Lukande ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Diagnostic Accuracy ◽  
Ovarian Carcinoma ◽  
East Africa ◽  
Response To Therapy ◽  
Tumor Type ◽  
Integrated Diagnosis ◽  
Resource Limited ◽  
Haematoxylin And Eosin Stain ◽  
Tumor Types

Abstract Background Ovarian cancer is a spectrum of several histologically distinct tumor types which differ in etiology, response to therapy and prognosis. In a resource-limited settings, the diagnosis of ovarian cancer can be challenging. This study describes the distribution of ovarian cancer tumor types in East Africa as well as assessing the diagnostic accuracy by using contemporary methods. Methods Data from 210 women identified from the records with a diagnosis of ovarian cancer in a period of 15 years were included. Two tissue microarrays were constructed and stained with 20 antibodies relevant to ovarian cancer subtyping. An integrated diagnosis was reached by the review of full Haematoxylin and Eosin stained sections, with consideration of immunohistochemical results. The integrated diagnoses were compared with the original diagnoses, and the degree of agreement was evaluated by percentage and Kappa statistics. Results The estimated rates of ovarian cancer were much lower in East Africa compared to a North American population from Alberta, Canada. There was a higher proportion of sex cord stromal tumors and germ cell tumors in the East African population. Diagnostic accuracy for main ovarian tumor type categories was substantial (Kappa 0.70), but only fair for specific ovarian carcinoma histotypes (Kappa 0.34). Poor Haematoxylin and Eosin stain was the main factor hindering correct diagnosis, which was not related to tissue processing. Conclusions In a resource- limited setting, where immunohistochemistry is not routinely carried out, diagnostic accuracy for the main categories of ovarian carcinoma is substantial and could be further improved by standardization of the basic Haematoxylin and Eosin stain.

scholarly journals Histological studies on the retina and cerebellum of Wistar rats treated with Arteether™

2014 ◽  
Vol 31 (01) ◽  
pp. 028-032
Author(s):  
A. Okunlola ◽  
C. Okunlola ◽  
C. Okani ◽  
O. Adewole ◽  
D. Ofusori ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Study Group ◽  
Group A ◽  
Cervical Dislocation ◽  
Nissl Stain ◽  
Group B ◽  
Haematoxylin And Eosin Stain ◽  
Therapeutic Doses ◽  
Cellular Components ◽  
Group D

Abstract Introduction: Arteether™, a derivative of artemisinin, is among the recent drugs that have given renewed hope for combating malarial menace. The present study investigated the effects of arteether™ on the histology of the retina and cerebellum of Wistar rats. Materials and Methods: Twenty adult albino Wistar rats weighing 150-200 g, were randomly divided into four groups (A, B, C and D) of five animals each and used for this study. Group A rats were given intramuscular (i.m.) arteether™ (3 mg/kg b.w.) daily for 3 days. Group B rats were given i.m. arteether™ (6 mg/kg b.w.) daily for 3 days. Group C rats were also given i. m. of arteether™ (3 mg/kg b. w.) daily for 3 days, and the same dose was repeated at two-weekly intervals for 4 further weeks; while Group D rats which received normal saline (0.9 % w/v, 3 ml/kg b.w.), served as controls. At the end of the experiment, the rats were sacrificed by cervical dislocation. The retina and cerebellum were excised and processed routinely for histopathology changes, using haematoxylin and eosin stain (H & E), as well as Nissl stain. Results: Results obtained showed normal cellular components of the retina and cerebellum in all groups, and no cyto-pathological changes were observed. Conclusion: Thus, this study showed that under light microscopic examination, therapeutic doses of arteether™ caused no significant cyto-pathologic changes in the retina and cerebellum of Wistar rats.

Electron microscopy of the oxynticopeptic cells of the gastric glands and the intestinal glands of the caecum of the guineapig

1992 ◽  
Vol 26 (1) ◽  
pp. 47-52 ◽  
Author(s):  
H. S. Bal ◽  
N. G. Ghoshal
Keyword(s):  
Electron Microscopy ◽  
Cell Types ◽  
Cell Type ◽  
Gastric Glands ◽  
Mature Adult ◽  
Pepsinogen Secretion ◽  
Pure Cell ◽  
Haematoxylin And Eosin Stain ◽  
Protein Secreting ◽  
Oxynticopeptic Cells

Histomorphology of the gastric and intestinal glands was investigated in 19 sexually mature, adult guineapigs by light and transmission electron microscopy. Gastric glands exhibited the cytological characteristics of oxynticopeptic cells capable of both hydrochloric acid (HCl) and pepsinogen secretion. In the literature, occurrence of oxynticopeptic cells in tbe proventriculus of the domestic fowl (Toner, 1963; Bell & Freeman, 1971) and in the gastric glands of frogs has been reported (Sedar, 1961; Patt & Patt, 1969; Forte & Forte, 1970). It has been claimed by other investigators (Herriot et al., 1938; Long, 1967) that simultaneous secretion of HCl and pepsinogen by a single, not completely differentiated 'pure' cell type, was highly effective for rapid conversion of the zymogen to active enzyme. Under the light microscope with haematoxylin and eosin stain, the protein secreting activity of gastric glands in guineapigs was masked by the HCl secreting activity, thus morphologically resembling the oxyntic cells. Therefore, different cell types, for example protein-secreting peptic cells and the acid-secreting oxyntic cells, could not be distinguished on the basis of their morphology and staining affinity. For histochemical evaluation of the sections with stains-all method, most cells in the gastric glands responded by a positive reaction to protein. Further, protein containing cells were seen in the intestinal glands of the guineapig caecum. The function of this cell type was correlated with caecotrophic food habits of this species.

scholarly journals Histochemical Localization of Bio-macromolecules in Cestode (Raillietina sp.) infected country fowls (Gallus domesticus) in India

2021 ◽  
Vol 8 (4) ◽  
pp. 457-460
Author(s):  
N C Patra ◽  
Arkaprava Mandal ◽  
S Pradhan ◽  
R N Hansda ◽  
Samiran Mondal ◽  
...  
Keyword(s):  
Growth Rate ◽  
Health Status ◽  
Lipid Droplet ◽  
Congo Red ◽  
Gallus Domesticus ◽  
The Body ◽  
Protein Source ◽  
Poultry Meat ◽  
Indian Country ◽  
Haematoxylin And Eosin Stain

In Indian GDP, veterinary sector plays a major role in contribution wherein poultry meat plays good protein source. But the health status is less studied in case of country fowl.Indian country fowl are highly infected with producing emaciation, reduced growth rate, highly morbidity with enteritis.After confirmation by gross and microscopy, samples undergone histopathology study using Haematoxylin and Eosin stain (H&E). Thereafter, the samples also processed for detection of carbohydrate by McManus method using PAS stain and mucoprotein was detected using congo red method.Adult possesses carbohydrate, protein and fat. Histologically the mature, immature and gravid segments were differentiated whereas the histochemical techniques qualifies the biomacromolecules. The lipid droplet showed a clear vacuole preferably in and around the periphery portion. The glycogen distributed in an even manner. Subsequently, the protein was deposited mostly at the periphery in the body surfaces as well as in the egg.

scholarly journals Environmental health hazard of arsenic on epididymis and ductus deferens in male Black Bengal Goats

2014 ◽  
Vol 11 (1) ◽  
pp. 103-110 ◽  
Author(s):  
MA Wares ◽  
MA Awal ◽  
M Nasrin ◽  
MNH Siddiqi
Keyword(s):  
Environmental Health ◽  
Histological Study ◽  
Health Hazard ◽  
Reproductive Organs ◽  
Control Group ◽  
Ductus Deferens ◽  
Length Width ◽  
Histomorphological Changes ◽  
Haematoxylin And Eosin Stain ◽  
Black Bengal Goat

Histomorphological changes of epididymis and ductus deferens in male Black Bengal goat due to arsenic were studied. A total of 12 male Black Bengal goats, in which 6 were collected from arsenic affected areas of Mymensingh district and another 6 were collected from hill tracts of Chittagong which were arsenic free.The goats were sacrificed by piercing carotid artery and the samples (Epididymis and Ductus deferens) were collected immediately. Samples were prepared and stained with haematoxylin and eosin stain technique to study the histology under light microscope. In the morphological study, measurement of length, width, breadth and weight of epididymis and ductus deferens were observed. The gross study revealed that there were slight variations in the gross morphology of epididymis and ductus deferens of arsenic affected Black Bengal goat, but this variation was statistically insignificant. In the histological study, arsenic affected goat showing increased thickness of epididymal covering (P<0.05) and trabeculae compared to control group of epididymis. The diameter of ductule efferentes of arsenic affected goat was narrower (P<0.01), width between intertubular space of ductuli efferentes was wider (P<0.01), lumen of ductules contain smaller in amount of spermatozoa compared to control group. The wall of the ductus deferens was thicker in arsenic affected goats than the control group (P<0.01). It may be concluded that environmental health hazard of arsenic might have adverse effects on the male reproductive organs. DOI: http://dx.doi.org/10.3329/jbau.v11i1.18220 J. Bangladesh Agril. Univ. 11(1): 103-110, 2013

scholarly journals Morphometric Identification, Gross and Histopathological Lesions of Eimeria Species in Japanese Quails (Coturnix coturnix japonica) in Zaria, Nigeria

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
H. A. Umar ◽  
I. A. Lawal ◽  
O. O. Okubanjo ◽  
A. M. Wakawa
Keyword(s):  
Developmental Stage ◽  
Developmental Stages ◽  
Eimeria Species ◽  
Shape Index ◽  
Molecular Techniques ◽  
Japanese Quails ◽  
Histopathological Lesions ◽  
Faecal Samples ◽  
Shape Indices ◽  
Haematoxylin And Eosin Stain

The objective of the study was to identify the species, gross and histopathological lesions of Eimeria in Japanese quails in Zaria. A total of 400 fresh faecal samples were collected and 10 quail birds were purchased from a quail farm. The faecal samples were processed using simple floatation technique. Oocysts shape indices of sporulated oocysts were determined. The intestines were observed for gross lesions and segments were analyzed using Giemsa stain and Haematoxylin and Eosin stain and then observed microscopically for the developmental stages of the parasite. Four species of Eimeria were identified in the study. Eimeria bateri of shape index of 1.36 conformed to the guidelines used while the other three Eimeria species with shape indices of 1.48, 1.03, and 1.40 were not confirmed. The main gross lesion seen was nonhaemorrhagic ballooning of the caeca. Intestinal scrapping smear revealed a developmental stage of the parasite (merozoites) in the jejunum. Histopathology also revealed a developmental stage (schizont) of the parasite in the caecum and desquamation of the epithelial lining with areas of necrosis. Further study is required using molecular techniques to properly identify the unknown species of Eimeria that were detected in the study.

scholarly journals Ovarian Cancer: Diagnostic accuracy and tumor types distribution in East Africa compared to North America

2020 ◽  
Author(s):  
Peter Fabian Rambau ◽  
Martin Köbel ◽  
Derek Tilley ◽  
Alex Mremi ◽  
Robert Lukande ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Diagnostic Accuracy ◽  
Ovarian Carcinoma ◽  
East Africa ◽  
Response To Therapy ◽  
Tumor Type ◽  
Integrated Diagnosis ◽  
Resource Limited ◽  
Haematoxylin And Eosin Stain ◽  
Tumor Types

Abstract Background: Ovarian cancer is a spectrum of several histologically distinct tumor types that differ in etiology, response to therapy, and prognosis. In resource-limited settings, the diagnosis of ovarian cancer can be challenging. This study describes the distribution of ovarian cancer tumor types in East Africa as well as assessing the diagnostic accuracy by using contemporary methods. Methods: Data from 210 women identified from the records with a diagnosis of ovarian cancer in a period of 15 years were included. Two tissue microarrays were constructed and stained with 20 antibodies relevant to ovarian cancer subtyping. An integrated diagnosis was reached by the review of full Haematoxylin and Eosin stained sections, with consideration of immunohistochemical results. The integrated diagnoses were compared with the original diagnoses, and the degree of agreement was evaluated by percentage and Kappa statistics. Results: Though limited by selection bias, the results suggest lower rates of ovarian cancer in East Africa compared to a North American population from Alberta, Canada. There was a higher proportion of sex cord stromal tumors and germ cell tumors in the East African population. Diagnostic accuracy for main ovarian tumor type categories was substantial (Kappa 0.70), but only fair for specific ovarian carcinoma histotypes (Kappa 0.34). Poor Haematoxylin and Eosin stain was the main factor hindering the correct diagnosis, which was not related to tissue processing. Conclusions: In a resource-limited setting, where immunohistochemistry is not routinely carried out, diagnostic accuracy for the main categories of ovarian carcinoma is substantial and could be further improved by standardization of the basic Haematoxylin and Eosin stain.

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