Direct and indirect immunofluorescence staining patterns in the diagnosis of paraneoplastic pemphigus

2016 ◽  
Vol 174 (4) ◽  
pp. 912-915 ◽  
Author(s):  
A.M. Poot ◽  
J. Siland ◽  
M.F. Jonkman ◽  
H.H. Pas ◽  
G.F.H. Diercks
Keyword(s):  
Indirect Immunofluorescence ◽  
Immunofluorescence Staining ◽  
Paraneoplastic Pemphigus ◽  
Indirect Immunofluorescence Staining

scholarly journals Optimized fixation of actin filaments for improved indirect immunofluorescence staining of rickettsiae

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Monika Danchenko ◽  
Lucia Csaderova ◽  
Pierre Edouard Fournier ◽  
Zuzana Sekeyova
Keyword(s):  
Indirect Immunofluorescence ◽  
Actin Filaments ◽  
Immunofluorescence Assay ◽  
Host Cells ◽  
Immunofluorescence Staining ◽  
Sensitive Detection ◽  
Filamentous Actin ◽  
Indirect Immunofluorescence Staining ◽  
Accurate Imaging

Abstract Objective The objective was to investigate fixative solutions: 3.7% formaldehyde, 4% paraformaldehyde, 4% paraformaldehyde in the cytoskeletal buffer and 4% paraformaldehyde in PHEM buffer (containing PIPES, HEPES, EGTA and MgCl2), applicable for immunofluorescence assay. Results Herein we optimized this serological technique, testing four fixative solutions, for the sensitive detection of rickettsial antigens, and preservation of intracellular structures of the host cells, particularly filamentous actin. Rickettsial antigens were presented equally well both with formaldehyde and all paraformaldehyde-based fixations, but only protocol with 4% paraformaldehyde in PHEM buffer allowed accurate imaging of actin filaments, and simultaneously allows monitoring of rickettsiae using actin-based motility during infection inside the host cells.

SEM study of cytoskeleton of osteoblasts immobilized on HA-ceramic during biocompatibility testing

1992 ◽  
Vol 50 (1) ◽  
pp. 784-785
Author(s):  
P. Fratssinet ◽  
C. Delga ◽  
P. Conte ◽  
N. Rouquet ◽  
M. Nadal
Keyword(s):  
Indirect Immunofluorescence ◽  
Chicken Embryo ◽  
Light Microscope ◽  
Immunofluorescence Staining ◽  
Transparent Material ◽  
Differentiated Cells ◽  
Sem Study ◽  
Indirect Immunofluorescence Staining ◽  
Biocompatibility Testing

Some standards for biocompatibility testing of biomaterials requires the determination of the cytoskeleton aspect of differentiated cells in contact with the material. It is usually determined by indirect immunofluorescence staining of cytoskeleton proteins and examination with a light microscope.This examination is difficult on non transparent material and the resolution is very poor. In order to study the interaction of osteoblast cytoskeleton with HA-macroporous ceramics, we grew chicken embryo osteoblasts on the material then we extracted cell membranes to expose the cytoskeleton proteins. They were then examined in SEM.

HLA-B27 typing by use of flow cytofluorometry.

1987 ◽  
Vol 33 (9) ◽  
pp. 1619-1623 ◽  
Author(s):  
J Albrecht ◽  
H A Müller
Keyword(s):  
Indirect Immunofluorescence ◽  
Hla Typing ◽  
Immunofluorescence Staining ◽  
Cytotoxicity Test ◽  
Hla B27 ◽  
Cell Surfaces ◽  
Flow Cytofluorometry ◽  
Indirect Immunofluorescence Staining ◽  
The Cross ◽  
Total Agreement

Abstract We describe the use of flow cytofluorometry to type lymphocytes for HLA-B27 antigens. We modified the cytotoxicity test of Terasaki and McClelland [Nature (London) 1964;204:998] for use with a flow cytofluorometer. In a second assay we used a commercially available monoclonal anti-HLA-B27 antibody and indirect immunofluorescence staining of cell surfaces. Although this antibody cross reacts with B7 and Bw22 antigens, the results for HLA-B27 and the cross-reacting antigens can be separated. Comparison of our results with those by a HLA-typing laboratory for 100 patients showed total agreement of the assigned typings.

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