Embryo production by intracytoplasmic injection of sperm retrieved from neonatal testicular tissue of Agu pigs after cryopreservation and grafting into nude mice

2020 ◽  
Vol 91 (1) ◽  
Author(s):  
Hiroyuki Kaneko ◽  
Kazuhiro Kikuchi ◽  
Nguyen Thi Men ◽  
Thanh Quang Dang‐Nguyen ◽  
Motoharu Oyadomari ◽  
...  
Keyword(s):  
Nude Mice ◽  
Testicular Tissue ◽  
Embryo Production ◽  
Intracytoplasmic Injection

280 PORCINE BLASTOCYSTS DERIVED FROM IN VITRO-MATURED OOCYTES INJECTED INTRACYTOPLASMICALLY WITH SPERM FROM TESTICULAR TISSUE XENOGRAFTED INTO NUDE MICE

2006 ◽  
Vol 18 (2) ◽  
pp. 247 ◽  
Author(s):  
K. Kikuchi ◽  
M. Nakai ◽  
N. Kashiwazaki ◽  
M. Ozawa ◽  
N. Maedomari ◽  
...  
Keyword(s):  
Nude Mice ◽  
New Technology ◽  
Testicular Tissue ◽  
Cell Number ◽  
Blastocyst Stage ◽  
Embryo Production ◽  
Vitro Fertilization ◽  
Male Gametes ◽  
Sperm Suspension

The utilization of spermatogonia from testicular tissue after xenografting into immuno-deficient mice should lead to new insights for the conservation of male gametes. However, successful embryo production using sperm cells from xenografted testicular tissues has been limited to rhesus monkeys (Honaramooz et al. 2004 Biol. Reprod. 70, 1500-1503). In the present study, the objective was to establish this new technology for pig conservation in combination with intracytoplasmic sperm injection. Testes were obtained from male piglets 6 to 15 days old, in which most of the germ cells were gonocytes; these were minced into pieces of approximately 1.5 � 1.5 � 1.5 mm. Approximately 20 fragments were transplanted under the back skin of castrated nude mice 5 to 8 weeks old. The testicular grafts were recovered between 125 and 192 days after xenografting, minced in Dulbecco's phosphate-buffered saline, and centrifuged several times, to serve as a sperm suspension. In vitro maturation of the recipient oocytes (Kikuchi et al. 2002 Biol. Reprod. 66, 1033-1041) and injection with an intact spermatozoon, followed by electrical stimulation at 1 h post-injection (Nakai et al. 2003 Biol. Reprod. 68, 1003-1008), were carried out. The putative zygotes were cultured in vitro for 6 days (Kikuchi et al. 2002), and were then fixed, stained, and assessed for embryonic development and quality. From a total of 27 mice that were xenografted with testicular tissues, spermatids and spermatozoa were obtained in 19 of the mice (70.4%). Most of the spermatozoa were matured morphologically, showing faint motility after release into the collection medium. From a total of 253 oocytes (four replications) that were injected with sperm, 63 (24.9 � 7.1%) oocytes developed to the blastocyst stage. The average total cell number was 41.9 � 3.9. These values are comparable to those in in vitro fertilization by frozen-thawed spermatozoa, resulting in developmental ability to piglets after embryo transfer (25.3% and 48.7 cells; Kikuchi et al. 2002). These results suggest the possibility of embryo production using porcine spermatozoa that are differentiated from gonocytes within the xenografts.

Production of sperm from porcine fetal testicular tissue after cryopreservation and grafting into nude mice

2017 ◽  
Vol 91 ◽  
pp. 154-162 ◽  
Author(s):  
Hiroyuki Kaneko ◽  
Kazuhiro Kikuchi ◽  
Nguyen Thi Men ◽  
Michiko Nakai ◽  
Junko Noguchi ◽  
...  
Keyword(s):  
Nude Mice ◽  
Testicular Tissue

Intracytoplasmic injection of spermatids retrieved from testicular tissue: influence of testicular pathology, type of selected spermatids and oocyte activation

1997 ◽  
Vol 12 (6) ◽  
pp. 1203-1213 ◽  
Author(s):  
P. Vanderzwalmen ◽  
H. Zech ◽  
A. Birkenfeld ◽  
M. Yemini ◽  
G. Bertin ◽  
...  
Keyword(s):  
Testicular Tissue ◽  
Oocyte Activation ◽  
Intracytoplasmic Injection

scholarly journals Xenotransplantation of testicular tissue into nude mice can be used for detecting leukemic cell contamination

2007 ◽  
Vol 22 (7) ◽  
pp. 1899-1906 ◽  
Author(s):  
M. Hou ◽  
M. Andersson ◽  
S. Eksborg ◽  
O. Soder ◽  
K. Jahnukainen
Keyword(s):  
Nude Mice ◽  
Leukemic Cell ◽  
Testicular Tissue

Recovery of porcine sperm from fetal testicular tissue xenografted into nude mice

2014 ◽  
Author(s):  
Kazuhiro Kikuchi ◽  
Junko Noguchi ◽  
Hiroyuki Kaneko
Keyword(s):  
Nude Mice ◽  
Testicular Tissue

scholarly journals Generation of Live Piglets for the First Time Using Sperm Retrieved from Immature Testicular Tissue Cryopreserved and Grafted into Nude Mice

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e70989 ◽  
Author(s):  
Hiroyuki Kaneko ◽  
Kazuhiro Kikuchi ◽  
Michiko Nakai ◽  
Tamas Somfai ◽  
Junko Noguchi ◽  
...  
Keyword(s):  
Nude Mice ◽  
Testicular Tissue ◽  
First Time

Normal reproductive development of pigs produced using sperm retrieved from immature testicular tissue cryopreserved and grafted into nude mice

2014 ◽  
Vol 82 (2) ◽  
pp. 325-331 ◽  
Author(s):  
Hiroyuki Kaneko ◽  
Kazuhiro Kikuchi ◽  
Fuminori Tanihara ◽  
Junko Noguchi ◽  
Michiko Nakai ◽  
...  
Keyword(s):  
Nude Mice ◽  
Reproductive Development ◽  
Testicular Tissue

Ultrastructure of NPC/HK1 cells heterotransplanted in athymic nude mice

1982 ◽  
Vol 40 ◽  
pp. 236-237
Author(s):  
E.C. Chew ◽  
C.L. Li ◽  
D.P. Huang ◽  
H.C. Ho ◽  
L.S. Mak ◽  
...  
Keyword(s):  
Cell Line ◽  
Nude Mice ◽  
Biopsy Specimen ◽  
Epithelial Cell Line ◽  
Present Communication ◽  
Recurrent Tumour ◽  
Athymic Nude Mice ◽  
Cell Line Establishment ◽  
Fine Structures ◽  
Well Differentiated

An epithelial cell line, NPC/HK1, has recently been established from a biopsy specimen of a recurrent tumour of the nasopharynx which was histologically diagnosed as a moderately to well differentiated squamous cell carcinoma. A definite decrease in the amount of tonofilaments and desmosomes in the NPC/HK1 cells during the cell line establishment was observed. The present communication reports on the fine structures of the NPC/HK1 cells heterotraneplanted in athymic nude mice.

Sequential detection of mRNA for the chemokine IL-8 and macrophages (F4/80) in human melanoma

1995 ◽  
Vol 53 ◽  
pp. 1008-1009
Author(s):  
C.D. Bucana ◽  
R. Sanchez ◽  
R. Singh ◽  
I.J. Fidler
Keyword(s):  
Tumor Cells ◽  
Nude Mice ◽  
Constitutive Expression ◽  
Metastatic Potential ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Angiogenic Factor ◽  
Infiltrating Cells ◽  
Immunoperoxidase Assay ◽  
Multifunctional Cytokine

The purpose of this study was to demonstrate by ISH the presence of IL-8 mRNA, and by immunohistochemistry (IHC) the presence of the chemokine IL-8 and the distribution of infiltrating macrophages in subcutaneous melanomas in the same tumor. IL-8 is a multifunctional cytokine produced by melanoma cells, activated macrophages and monocytes and it has been shown to be a growth and angiogenic factor for tumor cells. More recently it was shown that constitutive expression of IL-8 correlated directly with metastatic potential of human melanoma cells in nude mice. IL-8 content of a solid tumor as determined by Western blot analysis does not take into account the contribution of macrophages. Previous studies showed that murine tumors contain many infiltrating cells interspersed among tumor cells whereas human tumors growing in nude mice exhibit macrophages at the periphery or between tumor islands. In this study we demonstrate the expression of IL-8 and the distribution of macrophages by immunoperoxidase assay and IL-8 mRNA by ISH.

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