Effects of dietary lysozyme levels on growth performance, intestinal morphology, non-specific immunity and mRNA expression in weanling piglets

2015 ◽  
Vol 87 (3) ◽  
pp. 411-418 ◽  
Author(s):  
Yanrong Long ◽  
Sen Lin ◽  
Jiatao Zhu ◽  
Xiaoxue Pang ◽  
Zhengfeng Fang ◽  
...  
Keyword(s):  
Growth Performance ◽  
Mrna Expression ◽  
Intestinal Morphology ◽  
Specific Immunity ◽  
Weanling Piglets

scholarly journals Effects of vitamin B6 on the growth performance, intestinal morphology, and gene expression in weaned piglets that are fed a low-protein diet1

2020 ◽  
Vol 98 (2) ◽  
Author(s):  
Lanmei Yin ◽  
Jun Li ◽  
Huiru Wang ◽  
Zhenfeng Yi ◽  
Lei Wang ◽  
...  
Keyword(s):  
Growth Performance ◽  
Mrna Expression ◽  
Inflammatory Cytokines ◽  
Vitamin B6 ◽  
Animal Health ◽  
Dietary Supplementation ◽  
Experimental Period ◽  
Intestinal Morphology ◽  
Weaned Piglets ◽  
Cox 2

Abstract Vitamin B6 (VB6), which is an essential functional substance for biosome, plays an irreplaceable role in animal health. However, there are few studies that focus on the correlation between VB6 and intestinal health in weaned piglets. This study was conducted to investigate the effects of VB6 on the growth performance, intestinal morphology, and inflammatory cytokines and amino acid (AA) transporters mRNA expression in weaned piglets that are fed a low crude-protein (CP, 18%) diet. Eighteen crossbred piglets with initial body weights of 7.03 ± 0.17 kg (means ± SEM), weaned at 21-d age, were randomly assigned three diets with 0, 4, and 7 mg/kg VB6 supplementation, respectively. The experimental period lasted 14 days. Our results showed that there were no significant differences in growth performance, diarrhea rate, and biochemical parameters among the three treatments. In the jejunum, dietary VB6 supplementation did not affect the morphology and positive Ki67 counts. Dietary supplementation with 4 mg/kg VB6 decreased the mRNA expression of COX-2, IL-10, and TGF-β (P < 0.05). Dietary supplementation with 7 mg/kg VB6 increased the mRNA expression of SLC7A1, SLC7A6, SLC16A14, and SLC38A5 (P < 0.05) and 4 or 7 mg/kg VB6 decreased SLC36A1 mRNA expression (P < 0.05). In the ileum, VB6 supplementation did not affect positive Ki67 counts but significantly decreased villus area (P < 0.05) and tended to decrease villus height (P = 0.093). Dietary supplementation with 4 mg/kg VB6 had significantly increased the mRNA expression of IL-1β, TNF-α, COX-2, IL-10, and TGF-β (P < 0.05). Dietary supplementation with 4 or 7 mg/kg VB6 had significantly decreased SLC6A20, SLC7A1, SLC7A6, SLC16A14, and SLC38A5 mRNA expression (P < 0.05). These findings suggest that dietary supplementation of VB6 mainly down-regulated inflammatory cytokines and up-regulated AA transporters mRNA expression in jejunum, while up-regulated (4 mg/kg) inflammatory cytokines and down-regulated AA transporters mRNA expression in ileum, which may provide a reference for the intestinal development of weaned piglets that are fed a low-CP diet.

scholarly journals PSVI-40 Effects of dietary glucan on intestinal morphology, immunity response, barrier function and antioxidant capacity in weaning pigs

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 212-213
Author(s):  
xia xiong ◽  
Lvliang Wu ◽  
Yirui Shao ◽  
Jian zou ◽  
Yulong Yin
Keyword(s):  
Antioxidant Capacity ◽  
Growth Performance ◽  
Mrna Expression ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Intestinal Morphology ◽  
Control Group ◽  
Gut Health ◽  
Weaned Piglets ◽  
Weaning Piglets

Abstract Glucan has been studied as a potential alternative to antibiotics for animals in recent years. The aim of this study was to evaluate the effect of dietary glucan on growth performance and gut health of weaning piglets, which is a water-soluble extracellular ꞵ-glucan produced by Agrobacterium sp. ZX09. A total of 108 weaned piglets (21 d of age; 6.05 ± 0.36 kg) were randomly assigned (6 pens/diet; 18 piglets/pen) to 3 dietary treatments consisting of a basal diet (control group) or the basal diet supplemented with 20 ppm olaquindox or 200 ppm glucan for 14 days, respectively. The results showed that piglets fed with glucan had greater (P < 0.05) body weight and average daily gain than piglets in control group. Piglets fed with glucan or antibiotic had greater villus height to crypt depth ratio on duodenum compared with control group (P < 0.05). The mRNA expression of Claudin-1 on duodenum or ileum was higher (P < 0.05) in glucan group than that on the other groups. The mRNA expression of TLR4, MYD88 and NFκB on jejunum were lower (P < 0.05) in glucan or antibiotic group than those in control group. Dietary supplementation with glucan tended to increase the IL-10 and SIgA concentration on ileum (0.05 < P < 0.1). Dietary supplementation with glucan tended to increase the total antioxidant capacity on jejunum (P = 0.093). In conclusion, 200 ppm glucan or 20 ppm olaquindox can improve the growth performance of weaning piglets. The glucan may can accelerate the growth of weaned piglets by improving gut health. This research will provide guidance for the olaquindox alternative on growing piglets.

scholarly journals Eucommia ulmoides Flavones as Potential Alternatives to Antibiotic Growth Promoters in a Low-Protein Diet Improve Growth Performance and Intestinal Health in Weaning Piglets

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1998
Author(s):  
Daixiu Yuan ◽  
Jing Wang ◽  
Dingfu Xiao ◽  
Jiefeng Li ◽  
Yanhong Liu ◽  
...  
Keyword(s):  
Growth Performance ◽  
Intestinal Morphology ◽  
Coliform Bacteria ◽  
Feed Ratio ◽  
Growth Promoter ◽  
Eucommia Ulmoides ◽  
Intestinal Health ◽  
Villus Height ◽  
Low Protein ◽  
Weanling Piglets

Eucommia ulmoides flavones (EUF) have been demonstrated to attenuate the inflammation and oxidative stress of piglets. This study aimed to test whether EUF could be used as an alternative antibiotic growth promoter to support growth performance and maintain intestinal health in weanling piglets. Weaned piglets (n = 480) were assigned into three groups and fed with a low-protein basal diet (NC), or supplementation with antibiotics (PC) or 0.01% EUF (EUF). Blood, intestinal contents, and intestine were collected on days 15 and 35 after weaning. The results showed the PC and EUF supplementations increased (p < 0.05) body weight on day 35, average daily gain and gain: feed ratio from day 15 to day 35 and day 0 to day 35, whereas decreased (p < 0.05) the diarrhea index of weanling piglets. EUF treatment increased (p < 0.05) jejunal villus height: crypt depth ratio, jejunal and ileal villus height, and population of ileal lactic acid bacteria on day 15 but decreased (p < 0.05) the population of ileal coliform bacteria on day 15 and day 35. These findings indicated the EUF, as the potential alternative to in-feed antibiotic growth promoter, could improve growth performance and intestinal morphology, and decrease colonization of coliform bacteria and diarrhea index in weanling piglets.

scholarly journals Medium-chain TAG improve intestinal integrity by suppressing toll-like receptor 4, nucleotide-binding oligomerisation domain proteins and necroptosis signalling in weanling piglets challenged with lipopolysaccharide

2018 ◽  
Vol 119 (9) ◽  
pp. 1019-1028 ◽  
Author(s):  
Xiao Xu ◽  
Shaokui Chen ◽  
Haibo Wang ◽  
Zhixiao Tu ◽  
Shuhui Wang ◽  
...  
Keyword(s):  
Protein Expression ◽  
Mrna Expression ◽  
Nucleotide Binding ◽  
Intestinal Morphology ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Medium Chain ◽  
Intestinal Integrity ◽  
Weanling Piglets ◽  
Maize Oil

AbstractThis study was conducted to evaluate whether medium-chain TAG (MCT) could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury by regulating intestinal epithelial inflammatory response, as well as necroptosis. A total of twenty-four weanling piglets were randomly allotted to one of four treatments in a 2×2 factorial arrangement including diet type (5 % maize oil v. 4 % MCT+1 % maize oil) and immune stress (saline v. E. coli LPS). The piglets were fed diets containing maize oil or MCT for 21 d. On 21 d, piglets were injected intraperitoneally with saline or LPS. The blood and intestinal samples were collected at 4 h post injection. Supplementation with MCT improved intestinal morphology, digestive and barrier function, indicated by increased jejunal villus height, increased jejunal and ileal disaccharidases (sucrase and maltase) activities, as well as enhanced protein expression of claudin-1. Furthermore, the protein expression of heat-shock protein 70 in jejunum and the concentration of TNF-α in plasma were reduced in the piglets fed diets supplemented with MCT. In addition, MCT down-regulated the mRNA expression of toll-like receptor 4 (TLR4) and nucleotide-binding oligomerisation domain proteins (NOD) signalling-related genes in jejunum and ileum. Finally, MCT inhibited jejunal and ileal enterocyte necroptosis indicated by suppressed mRNA expression of the receptor-interacting protein 3 and mixed-lineage kinase domain-like protein. These results indicate that MCT supplementation may be closely related to inhibition of TLR4, NOD and necroptosis signalling pathways and concomitant improvement of intestinal integrity under an inflammatory condition.

scholarly journals Eucommia Ulmoides Flavones as Antibiotic Alternatives in a Low-protein Diet Improve Growth Performance and Intestinal Health in Weaning Piglets

2020 ◽  
Author(s):  
Daixiu Yuan ◽  
Jing Wang ◽  
Dingfu Xiao ◽  
Jiefeng Li ◽  
Yanhong Liu ◽  
...  
Keyword(s):  
Growth Performance ◽  
Average Daily Gain ◽  
Basal Diet ◽  
The Body ◽  
Intestinal Morphology ◽  
Coliform Bacteria ◽  
Eucommia Ulmoides ◽  
Intestinal Health ◽  
Low Protein ◽  
Weanling Piglets

Abstract Background Eucommia ulmoides flavones (EUF), have been demonstrated to attenuate the inflammation and oxidative stress of piglets. This study was designed to test whether EUF could be as an antibiotic alternative to support growth performance and maintain intestinal health in weanling-piglets. Weaned-piglets (n = 480) were assigned into 3 groups and fed with a low-protein basal diet (NC), or supplementation with antibiotics (PC) or 0.1‰ EUF (EUF). Blood, intestinal contents and intestine were collected on d-15 and d-35, respectively. Results The results showed that the body weight on d-35, average daily gain and gain : feed from d 15 to d 35 and d 0 to d 35 in piglets of PC and EUF treatments were higher than (p < 0.05) these in NC treatment, whereas PC and EUF treatments decreased (p < 0.05) the diarrhea index of weanling piglets. Dietary supplementation of EUF significantly enhanced (p < 0.05) the serum concentrations of total protein, alanine transaminase and the immunoglobulin G on d-15 of piglets compared to piglets in NC treatment. EUF supplementation increased (p < 0.05) the jejunal and ileal villus height and the population of ileal lactic acid bacteria on d-15 but remarkable decreased (p < 0.05) the population of ileal coliform bacteria on d-15 and d-35. Conclusion These findings indicated the antibiotic alternative capacity of EUF in piglets exhibiting by improving growth performance and intestinal morphology, decreasing colonization of coliform bacteria and diarrhea index in weanling piglets.

scholarly journals Exogenous dietary lysozyme improves the growth performance and gut microbiota in broiler chickens targeting the antioxidant and non-specific immunity mRNA expression

PLoS ONE ◽  
2017 ◽  
Vol 12 (10) ◽  
pp. e0185153 ◽  
Author(s):  
Mervat A. Abdel-Latif ◽  
Ali H. El-Far ◽  
Ahmed R. Elbestawy ◽  
Rania Ghanem ◽  
Shaker A. Mousa ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Growth Performance ◽  
Mrna Expression ◽  
Broiler Chickens ◽  
Specific Immunity

scholarly journals Dietary ZnO and arginine supplementation on the dynamic change of microbiota, intestinal morphology, and immune function of weaned pigs subjected to heat stress

2020 ◽  
Author(s):  
Se Young Yoon ◽  
SooJin Sa ◽  
Eun Seok Cho ◽  
HanSeo Ko ◽  
Ga Won Kim ◽  
...  
Keyword(s):  
Heat Stress ◽  
Growth Performance ◽  
Mrna Expression ◽  
Immune Status ◽  
Phase 1 ◽  
Dietary Supplementation ◽  
Intestinal Morphology ◽  
Weaned Pigs ◽  
Weaning Stress ◽  
Tnf Α

Abstract Background. Weaning stress is an economically important problem in the pigs, and the economic loss of the growth performance reduction is even more critical if the heat stress adds to the weaning stress. The supplementation of ZnO is an effective option in reducing the adverse effects of weaning time. This study aimed to investigate the effect of the L-arginine (Arg) inclusion and different doses of ZnO to determine the best dietary supplementation ratio on growth performance, intestinal microbiota and integrity, and immune status in weaned pigs. A total of 180 weaned pigs (28 day-old) were randomly allotted to six treatments with 6 replicate pens in each treatment and 5 pigs per pen. The dietary treatments were: control diet (Con; with 1.1% Arg and without ZnO supplementation); Con + 2500 ppm Zn as ZnO (P-Zn); Con + 1.6% Arg (ARG); Con + 500 ppm of Zn as ZnO + 1.6% Arg (ZnArg1); Con + 1000 ppm of Zn as ZnO + 1.6% Arg (ZnArg2); P-Zn + 1.6% Arg (ZnArg3). Results. The overall result showed that the inclusion of ZnArg3 significantly improved the average daily gain compared with the Con treatment. There was a reduction of feed intake in the Con diet compared with the ZnArg3 diet at phase 1 and overall. At phase 1, the weaned pigs in the ZnArg3 and P-Zn groups exhibited the decreased population of Clostridium spp. in the ileum compared with those of the Con group. In addition, a lower ileal Clostridium spp. population was detected in the ZnArg2 pigs compared with the Con pigs. At phase 2, the colonization of Clostridium spp. was higher in the Con and ARG treatments compared with ZnArg3 treatment. The weaned pigs fed the ZnArg1 and ZnArg3 diets showed a greater villus height of duodenum compared with the Con and P-Zn treatments. The count of eosinophil was significantly higher in the Con and ZnArg1 compared with the ZnArg2 and ZnArg3 treatments. The weaned pigs in the Con group showed increased mRNA expression of HSP27 in the liver compared with the P-Zn, ZnArg1, ZnArg2, and ZnArg3 groups. When fed the basal diet, weaned pigs exhibited enhanced mRNA expressions of IL-6 in the muscle compared with the ZnArg3 group. Dietary supplementation with ZnArg2 decreased the mRNA expressions of IFNγ in the muscle compared with the Con group. Supplementation with P-Zn, ZnArg1, ZnArg2, and ZnArg3 exhibited decreased mRNA expressions of TNF-α compared with the Con group. The mRNA gene expressions of IL-4 were decreased in the jejunum of P-Zn, ARG, ZnArg1, ZnArg2, and ZnArg3 weaned pigs compared with the Con group. The jejunum gene expression of TLR4 was upregulated in the Con and ARG treatments compared with the ZnArg1 and ZnArg3. The ZnArg1, ZnArg2, and ZnArg3 treatments showed a lower mRNA expression of TNF-α compared with the Con group. Conclusion. The Arg supplementation did not improve the growth performance, microbial composition, or immune status of weaned pigs but showed a similar growth performance when supplemented with 500 ppm Zn as ZnO compared with 2500 ppm Zn as ZnO.

scholarly journals Effects of Zinc Glycinate on Growth Performance, Serum Biochemical Indexes, and Intestinal Morphology of Yellow Feather Broilers

2021 ◽  
Author(s):  
Xiaoping Zhu ◽  
Xiuguo Shang ◽  
Guozhao Lin ◽  
Haojie Li ◽  
Xin Feng ◽  
...  
Keyword(s):  
Growth Performance ◽  
Mrna Expression ◽  
Average Daily Gain ◽  
Zinc Content ◽  
Basal Diet ◽  
Intestinal Morphology ◽  
Control Group ◽  
Transcriptional Level ◽  
Serum Biochemical ◽  
Morphology Parameters

Abstract The purpose of this study was to investigate the effects of zinc glycinate (Gly-Zn) on growth performance, serum biochemical index, intestinal morphology, and hepatic metallothionein (MT) mRNA expression in the liver of yellow feather broilers. A total of 540 18-day-old yellow feather broilers were randomly divided into three groups: control group (basal diet), ZnSO4 group (basal diet plus 60 mg Zn/kg from ZnSO4), and Gly-Zn group (basal diet plus 60 mg Zn/kg from zinc glycinate). Each treatment group had 6 replicates with 30 birds in each replicate. The experiment lasted for 42 days (18 to 59 days of age). The results showed that Gly-Zn supplementation significantly improved the average daily gain (ADG) and average daily feed intake (ADFI) of broilers during 18 to 39 days of age compared with that in the control group (P < 0.05) but not different from the ZnSO4 group. The Gly-Zn group had higher glutathione peroxidase (GSH-Px) (P < 0.05) and lower malondialdehyde (MDA) concentrations than the broilers in the control and ZnSO4 group. It was also observed that zinc content in the tibia of Gly-Zn group broilers was higher than the control and ZnSO4 group (P < 0.05). The results of intestinal morphology parameters showed that the Gly-Zn group significantly increased the villus height in duodenum and jejunum (P < 0.05) and decreased crypt depth in duodenum and ileum compared to the control group. However, there were no significant differences between the Gly-Zn group and ZnSO4 group in duodenum and ileum regarding intestinal morphology parameters. The Gly-Zn group significantly increased mRNA expression of MT in the liver than both control and ZnSO4 groups (P < 0.05). Collectively, the results indicated that supplementing 60 mg Zn/kg through zinc glycinate improved growth performance and serum indexes as well as intestinal morphology of yellow feather broilers. It also regulates MT gene expression more effectively than the ZnSO4 group at the transcriptional level.

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