Individuality in flounder ( Paralichthys olivaceus ): Differential responses of immune–endocrine during temperature stress in head kidney

2021 ◽  
Author(s):  
Xueshu Zhang ◽  
Mingzhe Yuan ◽  
Weiqun Lu
Keyword(s):  
Temperature Stress ◽  
Paralichthys Olivaceus ◽  
Head Kidney ◽  
Differential Responses

Differential responses of anthers of stress tolerant and sensitive wheat cultivars to high temperature stress

Planta ◽  
2021 ◽  
Vol 254 (1) ◽  
Author(s):  
Richard G. Browne ◽  
Song F. Li ◽  
Sylvana Iacuone ◽  
Rudy Dolferus ◽  
Roger W. Parish
Keyword(s):  
High Temperature ◽  
Temperature Stress ◽  
High Temperature Stress ◽  
Wheat Cultivars ◽  
Differential Responses

scholarly journals Expression analysis of tissue factor pathway inhibitors TFPI-1 and TFPI-2 in Paralichthys olivaceus and antibacterial and anticancer activity of derived peptides

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Guanghua Wang ◽  
Bing Xie ◽  
Yanli Su ◽  
Qinqin Gu ◽  
Dongfang Hao ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Tissue Factor ◽  
Micrococcus Luteus ◽  
Paralichthys Olivaceus ◽  
Expression Patterns ◽  
Head Kidney ◽  
Tissue Type ◽  
Pcr Analysis ◽  
Tissue Factor Pathway ◽  
The Brain

AbstractTissue factor pathway inhibitors (TFPI), including TFPI-1 and TFPI-2, are Kunitz-type serine protease inhibitors that mainly inhibit the blood coagulation induced by tissue factors. Previous reports on teleost proved TFPI play important roles in innate immunity. In this study, two TFPI (PoTFPI-1 and PoTFPI-2) molecules from Japanese flounder (Paralichthys olivaceus) were analyzed and characterized for their expression patterns, antibacterial and anticancer activities of the C-terminal derived peptides. Quantitative real time RT-PCR analysis shows that constitutive PoTFPI-1 expression occurred, in increasing order, in the brain, muscle, spleen, gills, head kidney, blood, intestine, heart, and liver; PoTFPI-2 was expressed, in increasing order, in the brain, gills, head kidney, muscle, intestine, spleen, liver, heart, and blood. Under the stimulation of fish pathogens, both PoTFPI-1 and PoTFPI-2 expressions increased significantly in a manner that depended on the pathogens, tissue type, and infection stage. Furthermore, C-terminal peptides TP25 and TP26, derived from PoTFPI-1 and PoTFPI-2, respectively, were synthesized and proved to be active against Micrococcus luteus (for TP25 and TP26) and Staphylococcus aureus (for TP25) via retardation effects on bacterial nucleic acids. In addition, TP25 and TP26 also displayed significant inhibitory effects on human colon cancer cell line HT-29. These results reveal that both PoTFPI-1 and PoTFPI-2 play important roles in host innate immunity. The antibacterial activity and anticancer cells function of TP25 and TP26 will add new insights into the roles of teleost TFPI.

scholarly journals Characteristics of Cyp11a during Gonad Differentiation of the Olive Flounder Paralichthys olivaceus

2018 ◽  
Vol 19 (9) ◽  
pp. 2641 ◽  
Author(s):  
Dongdong Liang ◽  
Zhaofei Fan ◽  
Yuxia Zou ◽  
Xungang Tan ◽  
Zhihao Wu ◽  
...  
Keyword(s):  
Treatment Group ◽  
Paralichthys Olivaceus ◽  
Expression Patterns ◽  
Head Kidney ◽  
Adenosine Monophosphate ◽  
Expression Level ◽  
Olive Flounder ◽  
High Temperature Treatment ◽  
Gonad Differentiation ◽  
Olive Flounder Paralichthys Olivaceus

The P450 side-chain cleavage enzyme, P450scc (Cyp11a) catalyzes the first enzymatic step for the synthesis of all steroid hormones in fish. To study its roles in gonads of the olive flounder Paralichthys olivaceus, an important maricultured fish species, we isolated the cyp11a genomic DNA sequence of 1396 bp, which consists of 5 exons and 4 introns. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) results indicated that the flounder cyp11a was exclusively expressed in gonad and head kidney tissues. Its expression level in the testis was higher than that in the ovary. According to the in situ hybridization patterns, cyp11a was mainly expressed in the Leydig cells of the testis, and the thecal cells of the ovary. Immunofluorescence analysis showed that Cyp11a was located in the cytoplasm of the cultured flounder testis cells. Further quantitative real-time PCR results presented the cyp11a differential expression patterns during gonad differentiation. Among different sampling points of the 17β-estradiol (E2, 5 ppm) treatment group, cyp11a expression levels were relatively high in the differentiating ovary (30 and 40 mm total length, TL), and then significantly decreased in the differentiated ovary (80, 100 and 120 mm TL, p < 0.05). The pregnenolone level also dropped in the differentiated ovary. In the high temperature treatment group (HT group, 28 ± 0.5 °C), the cyp11a expression level fluctuated remarkably in the differentiating testis (60 mm TL), and then decreased in the differentiated testis (80, 100 mm TL, p < 0.05). In the testosterone (T, 5 ppm) treatment group, the cyp11a was expressed highly in undifferentiated gonads and the differentiating testis, and then dropped in the differentiated testis. Moreover, the levels of cholesterol and pregnenolone of the differentiating testis in the HT and T groups increased. The expression level of cyp11a was significantly down-regulated after the cultured flounder testis cells were treated with 75 and 150 μM cyclic adenosine monophosphate (cAMP), respectively (p < 0.05), and significantly up-regulated after treatment with 300 μM cAMP (p < 0.05). Both nuclear receptors NR5a2 and NR0b1 could significantly up-regulate the cyp11a gene expression in a dosage dependent way in the testis cells detected by cell transfection analysis (p < 0.05). The above data provides evidence that cyp11a would be involved in the flounder gonad differentiation and development.

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