Characterization of a novel cathepsin D and its immunity response in noble scallop Chlamys nobilis with different total carotenoids content

2021 ◽  
Author(s):  
Hongkuan Zhang ◽  
Karsoon Tan ◽  
Huaiping Zheng
Keyword(s):  
Cathepsin D ◽  
Total Carotenoids ◽  
Chlamys Nobilis ◽  
Immunity Response

Chemical characterization of cathepsin D inhibitor from potatoes

1977 ◽  
Vol 42 (7) ◽  
pp. 2279-2286 ◽  
Author(s):  
L. Rupova ◽  
H. Keilová ◽  
V. Tomášek
Keyword(s):  
Cathepsin D ◽  
Chemical Characterization

Cloning and characterization of RAR s and RXR s from noble scallop Chlamys nobilis , a bivalve rich in carotenoids

2021 ◽  
Author(s):  
Karsoon Tan ◽  
Longhui Deng ◽  
Hongkuan Zhang ◽  
Hongyu Ma ◽  
Shengkang Li ◽  
...  
Keyword(s):  
Chlamys Nobilis

Purification and characterization of a digestive cathepsin D proteinase isolated from Tribolium castaneum Larvae (Herbst)

1996 ◽  
Vol 26 (1) ◽  
pp. 95-100 ◽  
Author(s):  
A. Blanco-Labra ◽  
N.A. Martinez-Gallardo ◽  
L. Sandoval-Cardoso ◽  
J. Delano-Frier
Keyword(s):  
Tribolium Castaneum ◽  
Cathepsin D ◽  
Purification And Characterization

Identification and characterization of a cathepsin D homologue from lampreys (Lampetra japonica)

2015 ◽  
Vol 49 (1) ◽  
pp. 149-156 ◽  
Author(s):  
Rong Xiao ◽  
Zhilin Zhang ◽  
Hongyan Wang ◽  
Yinglun Han ◽  
Meng Gou ◽  
...  
Keyword(s):  
Cathepsin D ◽  
Lampetra Japonica ◽  
Identification And Characterization

scholarly journals Characterization of a Macrophage-Specific Infectivity Locus (milA) of Legionella pneumophila

2000 ◽  
Vol 68 (1) ◽  
pp. 368-376 ◽  
Author(s):  
Omar S. Harb ◽  
Yousef Abu Kwaik
Keyword(s):  
Legionella Pneumophila ◽  
Cathepsin D ◽  
Laser Scanning ◽  
Laser Scanning Confocal Microscopy ◽  
Alveolar Epithelial Cells ◽  
Host Cells ◽  
Reading Frame ◽  
Alveolar Epithelial ◽  
Scanning Confocal Microscopy

ABSTRACT Legionella pneumophila has been shown to possess multiple genetic loci that play roles in its ability to survive within host cells. The mil (macrophage-specific infectivity loci) mutants of L. pneumophila exhibit a spectrum of defects in intracellular survival in and cytopathogenicity to macrophages and alveolar epithelial cells. This study characterizes one of themil mutants (GB111). Intracellular growth of GB111 in macrophages was approximately 100- to 1,000-fold less than that of AA100, the parental strain, at 24 and 48 h postinfection. This defect in turn corresponded to a defect in cytopathogenicity. Sequence analysis of the affected GB111 open reading frame (ORF) revealed it to encode a putative transport protein, and the ORF was designatedmilA. The phenotypic defect of the milA mutant was complemented with a PCR fragment containing only milA, indicating that the defect in GB111 was due to the disruption ofmilA. Intracellular trafficking of the mutant was examined by laser scanning confocal microscopy. The data showed that 50% of the GB111 phagosomes colocalized with the late endosomal/lysosomal marker LAMP-2 (2 and 4 h postinfection), while less than 10% of the AA100 phagosomes colocalized with this marker. On the other hand, over 80% of the GB111 phagosomes were similar to the AA100 phagosome in that they were devoid of LAMP-1 and cathepsin D, and they were colocalized with the endoplasmic reticulum (ER) marker BiP. However, the number of GB111 phagosomes that colocalized with BiP decreased to 50% 6 h postinfection compared to that of AA100, which remained constant (80% colocalization). Thus, compared to AA100, themilA mutation caused a defect in intracellular replication, which was associated with colocalization of the phagosome with LAMP-2 and BiP, while colocalization with LAMP-1 and cathepsin D was not affected.

scholarly journals Meloidogyne incognita: Molecular cloning and characterization of a cDNA encoding a cathepsin D-like aspartic proteinase

2009 ◽  
Vol 121 (2) ◽  
pp. 115-123 ◽  
Author(s):  
Rodrigo da Rocha Fragoso ◽  
Isabela Tristan Lourenço ◽  
João Aguiar Nogueira Batista ◽  
Osmundo Brilhante Oliveira-Neto ◽  
Maria Cristina Mattar Silva ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Meloidogyne Incognita ◽  
Cathepsin D ◽  
Aspartic Proteinase
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