Inflammatory response in swim bladder caused by Aeromonas hydrophila in tambaqui ( Colossoma macropomum Cuvier, 1816) supplemented with an autochthonous probiotic ( Bacillus cereus )

2021 ◽  
Author(s):  
Joel A. R. Dias ◽  
Lumar L. Alves ◽  
Márcia V. S. Couto ◽  
Francisco A. L. Barros ◽  
Carlos A. M. Cordeiro ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Bacillus Cereus ◽  
Aeromonas Hydrophila ◽  
Swim Bladder ◽  
Colossoma Macropomum

scholarly journals Acute inflammation and hematological response in Nile tilapia fed supplemented diet with natural extracts of propolis and Aloe barbadensis

2015 ◽  
Vol 75 (2) ◽  
pp. 491-496 ◽  
Author(s):  
G. Dotta ◽  
J. Ledic-Neto ◽  
ELT. Gonçalves ◽  
A. Brum ◽  
M. Maraschin ◽  
...  
Keyword(s):  
Immune Response ◽  
Inflammatory Response ◽  
Nile Tilapia ◽  
Acute Inflammation ◽  
Leukocyte Count ◽  
Acute Inflammatory Response ◽  
Swim Bladder ◽  
Aloe Barbadensis ◽  
Natural Extracts ◽  
Number Of Cells

This study evaluated the acute inflammatory response induced by carrageenin in the swim bladder of Nile tilapia supplemented with the mixture of natural extracts of propolis and Aloe barbadensis (1:1) at a concentration of 0.5%, 1% and 2% in diet during 15 days. Thirty-six fish were distributed into four treatments with three replicates: fish supplemented with 0.5% of admix of extracts of propolis and Aloe (1:1) injected with 500 µg carrageenin; fish supplemented with 1% of admix of extracts of propolis and Aloe (1:1) injected with 500 µg carrageenin; fish supplemented with 2% of admix of extracts of propolis and Aloe (1:1), injected with 500 µg carrageenin and unsupplemented fish injected with 500 µg carrageenin. Six hours after injection, samples of blood and exudate from the swim bladder of fish were collected. It was observed an increase in the leukocyte count in the swim bladder exudate of fish supplemented with extracts of propolis and Aloe injected with carrageenin. The most frequent cells were macrophages followed by granular leukocytes, thrombocytes and lymphocytes. Supplementation with propolis and Aloe to 0.5% caused a significant increase in the number of cells on the inflammatory focus mainly macrophages, cells responsible for the phagocytic activity in tissues, agent of innate fish immune response.

Involvement of cholinergic and purinergic systems during the inflammatory response caused by Aeromonas hydrophila in Rhamdia quelen

2016 ◽  
Vol 99 ◽  
pp. 78-82 ◽  
Author(s):  
Matheus D. Baldissera ◽  
Carine F. Souza ◽  
Pedro Henrique Doleski ◽  
Guerino B. Júnior ◽  
Agueda C. de Vargas ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Aeromonas Hydrophila ◽  
Rhamdia Quelen

scholarly journals Pengaruh pemberian mikrokapsul probiotik Bacillus cereus P22 dan Staphylococcus lentus L1k pada pakan terhadap kinerja pertumbuhan, respons imun, dan resistensi ikan lele, Clarias gariepinus Burchell 1822 yang diinfeksi Aeromonas hydrophila

2018 ◽  
Vol 17 (2) ◽  
pp. 143
Author(s):  
Lilik Setiyaningsih ◽  
Widanarni Widanarni ◽  
Angela Mariana Lusiastuti3 ◽  
Munti Yuhana
Keyword(s):  
Bacillus Cereus ◽  
Growth Performance ◽  
Aeromonas Hydrophila ◽  
Clarias Gariepinus ◽  
Bacterial Count ◽  
Negative Control ◽  
Total Bacterial Count ◽  
African Catfish ◽  
Feed Conversion ◽  
Significant Difference

The aimed of this research was to evaluate the effects of dietary probiotic microcapsules B.cereus P22 and S. lentus (L1k) at different dose and frequency on growth performance, immune response and resistance of African catfish infected with A. Hydrophila. Probiotics used in this study were B. cereus P22 and S. lentus L1k encapsulated by spray draying method. The research was carried out for 56 days with eight treatments and four replications. The treatments were K- (negative control), K+ (positive control), A (feed supplemented with 0,5% of microencapsulated probiotic, fedevery day), B (feed supplemented with 0,5% of microencapsulated probiotic, fed once every three days), C (feed supplemented with 1% of microencapsulated probiotic, fed every day), D (feed supplemented with 1% of microencap-sulated probiotic with an administration once every three days), E (feed with 2% of microencapsulated probiotic with an administration every day) and F (feed with 2% of microencapsulated probiotic with an administration once every three days). On day 42, all of the fish except K- were challenged by intramuscular injection of A. hydrophila (108 CFU ml-1). In 40 days after infection, there were no significant difference on survival rate (SR) between treatments (p> 0.05). Treatment E displayed the higher growth rate (4,54±0,02%), total probiotic B. cereus (P22) and S . lentus (L1k) (4.06± 0.09 log CFU g-1; 4.02±0.08 log CFU g-1) than other treatments; whereas treatment D showed the best feed conversion rasio(1.191±0.013), and treatment F offered the highest total bacterial count (7.11±0.53 log CFU g-1). An administration of 2% microencapsulated probiotic in every day frequency demonstrated the better growth performance, immune res-ponse and resistance of African catfish to A. hydrophila compare with other treatments. AbstrakPenelitian ini bertujuan untuk mengkaji dosis dan frekuensi pemberian mikrokapsul probiotik melalui pakan terhadap kinerja pertumbuhan, respons imun, dan resistensi ikan lele yang diinfeksi Aeromonas hydrophila. Penelitian ini meng-gunakan Bacillus cereus P22 dan Staphylococcus lentus L1k yang telah dienkapsulasi melalui metode spray draying. Penelitian ini dilaksankan selama 56 hari dengan delapan perlakuan dan empat ulangan, terdiri atas perlakuan K- (kon-trol negatif), K+ (kontrol positif), A (pakan+mikrokapsul probiotik dosis 0,5% frekuensi setiap hari), B (pakan+mikro-kapsul probiotik dosis 0,5% frekuensi tiga hari sekali), C (pakan+mikrokapsul probiotik dosis 1% frekuensi setiap hari), D (pakan+mikrokapsul probiotik dosis 1% frekuensi tiga hari sekali), E (pakan+mikrokapsul probiotik dosis 2% fre-kuensi setiap hari) dan F (pakan+mikrokapsul probiotik dosis 2% frekuensi tiga hari sekali). Ikan diuji tantang dengan A. hydrophila pada hari ke 42 dengan kepadatan 108 CFU ml-1 secara intramuscular (kecuali K- diinjeksi dengan phos-phate buffer saline). Setelah 40 hari pascainjeksi, laju sintasan tidak menunjukkan perbedaan yang signifikan antarperlakuan (p>0,05).Perlakuan E menunjukkan laju pertumbuhan (4,54±0,02%) dan total probiotik B. cereus (P22) dan S. lentus (L1k) (4,06±0,09 log CFU g-1; 4,02±0,08 log CFU g-1) tertinggi; sementara perlakuan D memberikan hasil nisbah konversi pakan terbaik (1,191±0,013), perlakuan F menunjukkan total bacterial count tertinggi (7,11±0,53 log CFU g-1). Hasil penelitian menunjukkan bahwa dosis 2% yang diberikan setiap hari memberikan hasil yang lebih baik dalam meningkatkan laju pertumbuhan, respons imun, dan resistensi ikan lele terhadap A. hydrophila. 

scholarly journals Desempenho produtivo e respostas fisiopatológicas de tambaquis alimentados com ração suplementada com β-glucano

2013 ◽  
Vol 48 (8) ◽  
pp. 899-905 ◽  
Author(s):  
Edsandra Campos Chagas ◽  
Fabiana Pilarski ◽  
Róberson Sakabe ◽  
Flávio Ruas de Moraes
Keyword(s):  
Aeromonas Hydrophila ◽  
Colossoma Macropomum

O objetivo deste trabalho foi avaliar o efeito do imunoestimulante β-glucano na dieta do tambaqui (Colossoma macropomum) sobre o desempenho produtivo, as respostas fisiológicas e imunológicas, e a resistência ao desafio com Aeromonas hydrophila. O experimento foi conduzido em delineamento inteiramente casualizado, em arranjo fatorial 5x2, com cinco níveis de β-glucano na dieta (0, 0,1, 0,2, 0,4 e 0,8%) e dois tempos de amostragem (antes e após o desafio com A. hydrophila), com três repetições. Os peixes (28,65±0,49 g; 12,14±0,07 cm) foram alimentados, por 60 dias, com dieta (28% de proteína bruta) suplementada com preparação comercial de β-glucano. Após o período de alimentação, avaliou-se o desempenho produtivo, e os peixes foram desafiados com A. hydrophila. Os parâmetros hematológicos e imunológicos (concentração e atividade de lisozima) foram avaliados antes e após o desafio bacteriano. Após o desafio bacteriano, observouse a ocorrência de anemia normocítica-normocrômica. A suplementação com β-glucano não alterou a concentração nem a atividade da lisozima; porém, a menor concentração de β-glucano (0,1%) favoreceu maior sobrevivência para a espécie quando desafiada com Aeromonas hydrophila. A suplementação de β-glucano não exerce influência sobre o desempenho produtivo e nem sobre os parâmetros hematológicos do tambaqui.

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