In vitro anthelminthic activity of Lippia  alba essential oil combined with silk fibroin against monogeneans of Colossoma   macropomum (Serrasalmidae)

2021 ◽  
Author(s):  
Marcos Tavares‐Dias ◽  
Ligia Rigôr Neves ◽  
Carliane Maria Guimarães Alves ◽  
Joziele Neves Nogueira ◽  
Fernando Batista Neves ◽  
...  
2021 ◽  
Author(s):  
Carliane Maria Guimarães Alves ◽  
Joziele Neves Nogueira ◽  
João Gabriel Rosário Luz ◽  
Francisco Célio Maia Chaves ◽  
Marcos Tavares‐Dias

2020 ◽  
Vol 141 (1) ◽  
pp. 55-65 ◽  
Author(s):  
Kamila Motta de Castro ◽  
Diego Silva Batista ◽  
Tatiane Dulcineia Silva ◽  
Evandro Alexandre Fortini ◽  
Sérgio Heitor Sousa Felipe ◽  
...  

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Emanuela Mesquita Porfírio ◽  
Hider Machado Melo ◽  
Antônio Matheus Gomes Pereira ◽  
Theodora Thays Arruda Cavalcante ◽  
Geovany Amorim Gomes ◽  
...  

In vitro antimicrobial and antibiofilm activities of the Lippia alba essential oil and its major components (citral and carvone) against Staphylococcus aureus were investigated. Essential oils (LA1EO, LA2EO, and LA3EO) were extracted from the aerial parts of three L. alba specimens by hydrodistillation and analyzed by gas chromatography coupled to a mass spectrometer. Minimum Inhibitory Concentrations (MIC) and Minimum Bacterial Concentration (MBC) were determined by the microdilution method. For the antibiofilm assays, the biomass formation in the biofilm was evaluated by the microtiter-plate technique with the crystal violet (CV) assay and the viability of the bacterial cells was analyzed. All oils and their major components presented antibacterial activity, and the lowest MIC and MBC values were 0.5 mg mL−1 when LA1EO and citral were used. Potential inhibition (100%) of S. aureus biofilm formation at the concentration of 0.5 mg mL−1 of all EOs was observed. However, the elimination of biofilm cells was confirmed at concentrations of 1 mg mL−1, 2 mg mL−1, 2 mg mL−1, and 0.5 mg mL−1 for LA1EO, LA2EO, LA3EO, and citral, respectively. The results obtained in the present research point to the promising antibacterial and antibiofilm potential of L. alba EOs against S. aureus, a species of recognized clinical interest.


2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1986290
Author(s):  
Amner Muñoz-Acevedo ◽  
María C. González ◽  
Juan D. Rodríguez ◽  
Yurina Sh. De Moya

Lippia alba is a plant widely studied due to both chemical diversity and bioactivities related to its ethnobotanical uses. In this work, the composition of the volatile secondary metabolites (volatile fractions/essential oil, EO) of the flower/leaves of L. alba (from northern region of Colombia) was determined by solid phase micro-extraction/distillation-solvent extraction/microwave-hydrodistillation/gas chromatography-mass spectrometry (MWHD/GC-MS), along with some in vitro biological properties (cytotoxicity and acetylcholinesterase enzyme [AChe] inhibition) from leaf EO. Outstanding results were found: (i) cis-piperitone oxide (~13%-46%), germacrene D (~11%-30%), and limonene (~10%-22%) characterized the volatile secondary metabolites from different parts of the plant; (ii) leaf EO showed a moderate hemolytic activity (HC50: 580 ± 1 µg/mL), a significant cytotoxicity on lymphocytes (LC50: 127 ± 3 µg/mL), a high cytotoxicity on HEp2 cell line (LC50: 38 ± 2 µg/mL), and a moderate inhibitory effect on AChE (IC50: 28 ± 2 µg/mL). Based on these results, a new chemovar of L. alba is reported (represented by cis-piperitone oxide) along with its promising cytotoxic and AChE inhibiting properties.


Aquaculture ◽  
2016 ◽  
Vol 465 ◽  
pp. 374-379 ◽  
Author(s):  
Artur Cedraz Sena ◽  
Rafael Rivas Teixeira ◽  
Erica Lima Ferreira ◽  
Berta Maria Heinzmann ◽  
Bernardo Baldisserotto ◽  
...  

2020 ◽  
Vol 140 (3) ◽  
pp. 593-603
Author(s):  
Kamila Motta de Castro ◽  
Diego Silva Batista ◽  
Tatiane Dulcineia Silva ◽  
Evandro Alexandre Fortini ◽  
Sérgio Heitor Sousa Felipe ◽  
...  

2017 ◽  
Vol 41 (3) ◽  
pp. 443-449 ◽  
Author(s):  
D S S Valentim ◽  
J L Duarte ◽  
A E M F M Oliveira ◽  
R A S Cruz ◽  
J C T Carvalho ◽  
...  

2015 ◽  
Vol 87 (1) ◽  
pp. 95-100 ◽  
Author(s):  
FERNANDO J. SUTILI ◽  
MAURO A. CUNHA ◽  
ROSANGELA E. ZIECH ◽  
CARINA C. KREWER ◽  
CARLA C. ZEPPENFELD ◽  
...  

In vitro and in vivo activity of the Lippia alba essential oil (EO) against Aeromonas sp. was evaluated. In the in vitro assay the minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) of EO for Aeromonas cells were determined using the microdilution method. Twenty five strains of Aeromonassp. isolated from infected fish obtained from local fish farms were used. MIC and MBC values were 2862 and 5998 µg mL-1 for L. alba EO and 0.5 and 1.2 µg mL-1for gentamicin, respectively. In the in vivo assay silver catfish juveniles (Rhamdia quelen) (7.50 ± 1.85 g and 10.0 ± 1.0 cm) with typical injuries associated to Aeromonas infection were divided into four treatments (in triplicate n=10): untreated fish (negative control), 10 mg L-1 of gentamicin, and 20 or 50 µL L-1 of EO. Fish were maintained in aerated 20 L plastic boxes. After 10 days survival of silver catfish infected with Aermonas sp. and treated with essential oil (50 µL L-1) was greater than 90%.


2016 ◽  
Vol 88 (2) ◽  
pp. 999-1010 ◽  
Author(s):  
ELISA Z. TOMAZONI ◽  
MÁRCIA R. PANSERA ◽  
GABRIEL F. PAULETTI ◽  
SIDNEI MOURA ◽  
RUTE T.S. RIBEIRO ◽  
...  

Several volatile natural compounds produced by plant secondary metabolism have been proven to present antimicrobial action, enabling their use in phytopathogen control. They also present low environmental impact when compared to conventional pesticides. Essential oils contain these compounds and can be found in several plant species, such as Lippia alba (Mill.) N.E. Brown (Verbenaceae). Essential oils of four chemotypes of L. alba, characterized by their major compounds, namely camphor, citral, linalool and camphor/1,8-cineole, were tested against the phytopathogen Alternaria solani Sorauer (Pleosporaceae), which causes early blight on tomatoes and is responsible for great economic losses regarding production. Essential oils antifungal action was tested in vitro using potato dextrose agar medium with essential oil concentrations at 0.1, 0.5, 1.0, 1.5 and 2.0 µL mL-1. The chemotype that had the best performance was citral, showing significant inhibition compared to the others, starting at the 0.5 µL mL-1 concentration. The essential oil belonging to the linalool chemotype was efficient starting at the 1.5 µL mL-1 concentration. Conversely, the camphor chemotype did not show any action against the phytopathogen. Moreover, the essential oils had no remarkable effect on tomato germination and growth. In conclusion, these essential oils presented fungicidal action against A. solani.


Aquaculture ◽  
2016 ◽  
Vol 452 ◽  
pp. 107-114 ◽  
Author(s):  
Bruna Viana Soares ◽  
Lígia Rigôr Neves ◽  
Marcos Sidney Brito Oliveira ◽  
Francisco Célio Maia Chaves ◽  
Márcia Kelly Reis Dias ◽  
...  

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