Effects of culture methods on the nutrient levels, physiological characteristics and intestinal microbiota of the innkeeper worm Urechis unicinctus

Effects of substrate on the physiological characteristics and intestinal microbiota of Echiura worm (Urechis unicinctus) juveniles

Aquaculture ◽

10.1016/j.aquaculture.2020.735710 ◽

2021 ◽

Vol 530 ◽

pp. 735710 ◽

Cited By ~ 1

Author(s):

Wenbo Chen ◽

Saisai Zhang ◽

Yang Sun ◽

Bin Tian ◽

Luojia Song ◽

...

Keyword(s):

Intestinal Microbiota ◽

Physiological Characteristics ◽

Urechis Unicinctus

Download Full-text

Movement and Fixation of Intestinal Microbiota after Administration of Human Feces to Germfree Mice

Applied and Environmental Microbiology ◽

10.1128/aem.71.6.3171-3178.2005 ◽

2005 ◽

Vol 71 (6) ◽

pp. 3171-3178 ◽

Cited By ~ 34

Author(s):

Ryoko Kibe ◽

Mitsuo Sakamoto ◽

Hiroshi Yokota ◽

Hiroki Ishikawa ◽

Yuji Aiba ◽

...

Keyword(s):

Intestinal Microbiota ◽

Intestinal Bacteria ◽

Rrna Gene ◽

Culture Methods ◽

Total Peak Area ◽

Human Intestinal Microbiota ◽

Human Intestinal Bacteria ◽

The Family ◽

Mouse Intestine ◽

Human Flora

ABSTRACT Human flora-associated (HFA) mice have been considered a tool for studying the ecology and metabolism of intestinal bacteria in humans, although they have some limitations as a model. Shifts in dominant species of microbiota in HFA mice after the administration of human intestinal microbiota was revealed by 16S rRNA gene sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses. Characteristic terminal restriction fragments (T-RFs) were quantified as the proportion of total peak area of all T-RFs. Only the proportion of the T-RF peak at bp 366, identified as the Gammmaproteobacteria group and the family Coriobacteriaceae, was reduced in this study. Increased T-RFs over time at bp 56, 184, and 196 were affiliated with the Clostridium group. However, most of the isolated bacteria with unique population shifts were phylotypes. The vertical transmission of the intestinal microbiota of the mouse offspring was also investigated by dendrogram analysis derived from the similarity of T-RFLP patterns among samples. As a result, the intestinal microbiota of HFA mice and their offspring reflected the composition of individual human intestinal bacteria with some modifications. Moreover, we revealed that human-derived lactobacilli (HDL), which have been considered difficult to colonize in the HFA mouse intestine in previous studies based on culture methods, could be detected in the HFA mouse intestine by using a lactic acid bacterium-specific primer and HDL-specific primers. Our results indicate that the intestinal microbiota of HFA mice represents a limited sample of bacteria from the human source and are selected by unknown interactions between the host and bacteria.

Download Full-text

Effects of stocking density on the growth performance, physiological response and intestinal microbiota of juvenile Echiura worms ( Urechis unicinctus )

Aquaculture Research ◽

10.1111/are.14741 ◽

2020 ◽

Vol 51 (10) ◽

pp. 3983-3992 ◽

Cited By ~ 1

Author(s):

Wenbo Chen ◽

Saisai Zhang ◽

Yan Xu ◽

Yang Sun ◽

Luojia Song ◽

...

Keyword(s):

Growth Performance ◽

Intestinal Microbiota ◽

Physiological Response ◽

Stocking Density ◽

Urechis Unicinctus

Download Full-text

Relationship between the composition of the digestive microbiota and the concentrations of opportunistic pathogens in intensive care patients

10.1101/2020.05.12.090373 ◽

2020 ◽

Author(s):

Candice Fontaine ◽

Laurence Armand-Lefèvre ◽

Mélanie Maignan ◽

Anissa Nazimoudine ◽

Jean-François Timsit ◽

...

Keyword(s):

Intensive Care ◽

Intestinal Microbiota ◽

Multidrug Resistant ◽

Opportunistic Pathogens ◽

Culture Methods ◽

Intensive Care Patients ◽

Endotracheal Aspiration ◽

Hospital Patients ◽

Medical Intensive Care ◽

Negative Link

AbstractObjectiveInfections caused by multidrug-resistant Gram-negative bacilli (MDR-GNB) are a major issue in intensive care. The intestinal and oropharyngeal microbiota being the reservoir of MDR-GNB. Our main objective was to assess the link between the composition of the intestinal and tracheal microbiota and colonization by MDR-GNB.MethodsWe performed a 2-month prospective, monocentric cohort study in the medical intensive care unit of our hospital. Patients ventilated >3 days and spontaneously passing faeces were included. A faecal sample and an endotracheal aspiration (EA) were collected twice a week. MDR-GNB but also Enterococcus faecium and yeasts (as potential dysbiosis surrogate markers) were detected by culture methods. The composition of the intestinal and tracheal microbiota was assessed by 16S profiling.ResultsWe collected 62 couples of faeces and EA from 31 patients, including 18 faeces and 9 EA positive for MDR-GNB. We did not observe a link between the diversity and the richness of the intestinal microbiota and the MDR-GNB intestinal relative abundance (RA). Conversely, we observed a negative link between the intestinal diversity and richness and the RA of Enterococcus spp. (p<0.001).ConclusionThe intestinal MDR-GNB RA was not associated to the diversity nor the richness of the intestinal microbiota, but that of Enterococcus spp. was.

Download Full-text