Cultivable autochthonous bacteria of the intestinal mucosa of Arapaima gigas (Pisces: Arapaimidae) with probiotic potential

2020 ◽  
Author(s):  
Aldo Aparecido Proietti‐Junior ◽  
Luciana Sampaio Lima ◽  
Verônica Duarte Gonçalves ◽  
Bruno Rocha Pribul ◽  
Dália Prazeres Rodrigues ◽  
...  
2017 ◽  
Vol 6 (5) ◽  
pp. 50
Author(s):  
Patience Chisa Obinna-Echem

Two strains of Lactobacillus plantarum isolated from akamu a Nigerian fermented maize food were investigated for probiotic potential based on: adhesion to hydrocarbons (hydrophobicity), porcine mucin and epithelial cell models. Gelatinase and haemolytic activities of the L. plantarum isolates were also studied. Adhesions to mono polar solvents (>22%) were significantly (p≤0.05) higher than the n-alkanes (<13%) with significant maximal affinity (35%) for chloroform an acidic solvent. The general order of affinity was chloroform > ethyl acetate > hexadecane > hexane. NGL7 had significantly (p≤0.05) the highest affinity for all the solvents. Both L. plantarum strains had significant adhesions to porcine mucin (≥6.51 Log10 CFU mL-1) after 2 h at 37oC. Viable counts on Caco-2 cells were 5.13 and 5.53 Log10 CFU mL-1 for NGL7 and NGL5 respectively. The L. plantarum strains possessed significant adhesion abilities: adhesion to hydrocarbons, porcine mucin and Caco-2 cells with no gelatinase and haemolytic activity. This suggested that the L. plantarum strains isolated from the Nigerian fermented maize food -akamu would be able to adhere to the intestinal mucosa and epithelial cells for beneficial health effects without posing any risk. 


2016 ◽  
Vol 9 (1) ◽  
pp. 12-21 ◽  
Author(s):  
Ankita Nandi ◽  
Suhas Kumar Dan ◽  
Goutam Banerjee ◽  
Pinki Ghosh ◽  
Koushik Ghosh ◽  
...  

2021 ◽  
Author(s):  
Suzana Kotzent ◽  
Silvia Umeda Gallani ◽  
Gustavo Moraes Ramos Valladão ◽  
Lindomar de Oliveira Alves ◽  
Fabiana Pilarski

Author(s):  
R. B. Moyes ◽  
R. E. Droleskey ◽  
M. H. Kogut ◽  
J. R. DeLoach

Salmonella enteritidis (SE) is of great concern to the poultry industry due to the organism's ability to penetrate the intestinal mucosa of the laying hen and subsequently colonize the ovaries and yolk membrane. The resultant subclinical infection can lead to SE infection of raw eggs and egg products. Interference with the ability of the organism to invade has been linked to the activation and recruitment of inflammatory polymorphonuclear cells, heterophils, to the lamina propria of the intestinal tract.Recently it has been established that heterophil activation and increased resistance to SE organ invasion can be accomplished by the administration of SE-immune lymphokines (SE-ILK) obtained from supernatants of concanavalin-A stimulated SE immune T lymphocytes from SE hyperimmunized hens. Invasion of SE into the lamina propria provides a secondary signal for directing activated heterophils to the site of SE invasion.


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