Expression and functional characterization of glutamine synthetase from giant freshwater prawn ( Macrobrachium rosenbergii ) under osmotic stress

2019 ◽  
Vol 50 (9) ◽  
pp. 2635-2645 ◽  
Author(s):  
Zhijie Lu ◽  
Zhendong Qin ◽  
Vijayaraman Sarath Babu ◽  
Chengkai Ye ◽  
Guomao Su ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Osmotic Stress ◽  
Functional Characterization ◽  
Macrobrachium Rosenbergii ◽  
Freshwater Prawn ◽  
Giant Freshwater Prawn

scholarly journals Osmoregulation of glutamine synthetase from Giant freshwater prawn (Macrobrachium rosenbergii) under osmotic stress

2019 ◽  
Author(s):  
Zhijie Lu ◽  
Zhendong Qin ◽  
V Sarath Babu ◽  
Chengkai Ye ◽  
Guomao Su ◽  
...  
Keyword(s):  
Amino Acid ◽  
Glutamine Synthetase ◽  
Osmotic Stress ◽  
Mrna Expression ◽  
Macrobrachium Rosenbergii ◽  
Amino Acid Sequences ◽  
Post Treatment ◽  
Freshwater Prawn ◽  
Giant Freshwater Prawn ◽  
Key Enzyme

AbstractGlutamine synthetase is a key enzyme that catalyzes the biosynthesis of glutamine (Gln) from glutamate and ammonia. Gln a vital amino acid acts as a precursor for protein synthesis and also assist in ammonia repressor and a key osmoregulators in aquatics. Here, we report the cloning and characterization of the GS gene from Macrobrachium rosenbergii (Mr-GS). The complete nucleotide and deduced amino acid sequences were determined that phylogenetically shared highest identity with other crustaceans. GS mRNA was differentially expressed in 6 different tissues, with high to low order as muscle > gills > heart > stomach > brain > haemolymph. Mr-GS expression and the glutamine concentrations were analyzed in the gills and muscle tissues of prawn under hyper/hypo-osmotic stress conditions. Under hyper-osmotic stress, the mRNA expression of Mr-GS was significantly increased in both gills and muscle at 3, 6 and 12 h post-treatment with 2.54, 4.21 and 10.83 folds, and 11.66, 17.97 and 45.92 folds, respectively. Protein analysis by western blot (WB) and Immunohistochemistry (IHC) further confirmed the Mr-GS expression was increased at 12 h post treatment. On the other hand, under hypo-osmotic stress, the mRNA expression of Mr-GS was also significantly increased in both gills and muscle at 3, 6 and 12 h post treatment with 1.63, 3.30 and 3.52 folds, and 4.06, 42.99 and 26.69 folds, respectively. Furthermore, under hyperosmotic stress, Gln concentration was increased in both gills and muscle at 6 and 12 h post treatment with 1.83, 2.02 folds, and 1.41, 1.29 folds, respectively. While, under hypo-osmotic stress, Gln concentration was increased in both gills and muscle at 3, 6 and 12 h post treatment with 3.99, 3.40, 2.59 folds, and 1.72, 1.83, 1.80 folds, respectively. Taken together, these results suggest that Mr-GS might play a key role in osmoregulation in M. rosenbergii.

scholarly journals Characterization of prostanoid pathway and the control of its activity by the eyestalk optic ganglion in the female giant freshwater prawn, Macrobrachium rosenbergii

Heliyon ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. e05898
Author(s):  
Tipsuda Thongbuakaew ◽  
Chanudporn Sumpownon ◽  
Attakorn Engsusophon ◽  
Napamanee Kornthong ◽  
Charoonroj Chotwiwatthanakun ◽  
...  
Keyword(s):  
Macrobrachium Rosenbergii ◽  
Freshwater Prawn ◽  
Giant Freshwater Prawn ◽  
Optic Ganglion

Isolation and characterization of microsatellite loci in giant freshwater prawn, Macrobrachium rosenbergii

2008 ◽  
Vol 10 (5) ◽  
pp. 1473-1475 ◽  
Author(s):  
Shripathi Bhat ◽  
Alok Patel ◽  
Paramananda Das ◽  
Prem K. Meher ◽  
Bidu R. Pillai ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Macrobrachium Rosenbergii ◽  
Freshwater Prawn ◽  
Giant Freshwater Prawn ◽  
Isolation And Characterization

Cloning and characterization of two different ficolins from the giant freshwater prawn Macrobrachium rosenbergii

2014 ◽  
Vol 44 (2) ◽  
pp. 359-369 ◽  
Author(s):  
Xiao-Wen Zhang ◽  
Xian-Wei Wang ◽  
Ying Huang ◽  
Kai-Min Hui ◽  
Yan-Ru Shi ◽  
...  
Keyword(s):  
Macrobrachium Rosenbergii ◽  
Freshwater Prawn ◽  
Giant Freshwater Prawn

scholarly journals Identification and Characterization of Differentially Expressed Transcripts in the Gills of Freshwater Prawn (Macrobrachium rosenbergii) under Salt Stress

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Hirak Kumar Barman ◽  
Swagat Kumar Patra ◽  
Varsha Das ◽  
Shibani Dutta Mohapatra ◽  
Pallipuram Jayasankar ◽  
...  
Keyword(s):  
Saline Water ◽  
Macrobrachium Rosenbergii ◽  
Freshwater Prawn ◽  
Salinity Acclimation ◽  
Important Species ◽  
Giant Freshwater Prawn ◽  
Wide Range ◽  
Rapid Amplification ◽  
Identification And Characterization

The giant freshwater prawn,Macrobrachium rosenbergii,is an economically important species. It is a euryhaline shrimp, surviving in wide-range salinity conditions. A change in gene expression has been suggested as an important component for stress management. To better understand the osmoregulatory mechanisms mediated by the gill, a subtractive and suppressive hybridization (SSH) tool was used to identify expressed transcripts linked to adaptations in saline water. A total of 117 transcripts represented potentially expressed under salinity conditions. BLAST analysis identified 22% as known genes, 9% as uncharacterized showing homologous to unannotated ESTs, and 69% as unknown sequences. All the identified known genes representing broad spectrum of biological pathways were particularly linked to stress tolerance including salinity tolerance. Expression analysis of 10 known genes and 7 unknown/uncharacterized genes suggested their upregulation in the gills of prawn exposed to saline water as compared to control indicating that these are likely to be associated with salinity acclimation. Rapid amplification of cDNA ends (RACE) was used for obtaining full-length cDNA of MRSW-40 clone that was highly upregulated during salt exposure. The sequenced ESTs presented here will have potential implications for future understanding about salinity acclimation and/or tolerance of the prawn.

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