Simultaneous effect of sex and dose on efficacy of clove oil, tricaine methanesulfonate, 2-phenoxyethanol and propofol as anaesthetics in guppies,Poecilia reticulata(Peters)

2018 ◽  
Vol 49 (6) ◽  
pp. 2140-2146 ◽  
Author(s):  
Olga Mitjana ◽  
Cristina Bonastre ◽  
María Teresa Tejedor ◽  
Laura Garza ◽  
Jaime Esteban ◽  
...  
Keyword(s):  
Poecilia Reticulata ◽  
Clove Oil ◽  
Simultaneous Effect ◽  
Tricaine Methanesulfonate

Comparative efficacy of tricaine methanesulfonate and clove oil for use as anesthetics in red pacu (Piaractus brachypomus)

2001 ◽  
Vol 62 (3) ◽  
pp. 337-342 ◽  
Author(s):  
Kurt K. Sladky ◽  
Clifford R. Swanson ◽  
Michael K. Stoskopf ◽  
Michael R. Loomis ◽  
Gregory A. Lewbart
Keyword(s):  
Clove Oil ◽  
Comparative Efficacy ◽  
Tricaine Methanesulfonate

The Use of Tricaine Methanesulfonate, Clove Oil, Metomidate, and 2-Phenoxyethanol for Anesthesia Induction in Alewives

2015 ◽  
Vol 78 (1) ◽  
pp. 84-91 ◽  
Author(s):  
David L. Berlinsky ◽  
Mark T. Watson ◽  
Matthew A. DiMaggio ◽  
Timothy S. Breton
Keyword(s):  
Anesthesia Induction ◽  
Clove Oil ◽  
Tricaine Methanesulfonate

scholarly journals The acute toxicity of clove oil to fish Danio rerio and Poecilia reticulata

2011 ◽  
Vol 80 (3) ◽  
pp. 305-308 ◽  
Author(s):  
Petra Doleželová ◽  
Stanislava Mácová ◽  
Lucie Plhalová ◽  
Vladimíra Pištěková ◽  
Zdeňka Svobodová
Keyword(s):  
Acute Toxicity ◽  
Fish Species ◽  
Danio Rerio ◽  
Poecilia Reticulata ◽  
Juvenile Fish ◽  
Probit Analysis ◽  
Model Organisms ◽  
Toxicity Tests ◽  
Clove Oil ◽  
Oil Composition

Clove oil (active substance eugenol) is an anaesthetic used in aquaculture for stress prevention and prevention of mechanical damage during veterinary procedures. The aim of this study was to determine the acute toxicity of clove oil in two aquarium fish species - zebrafish (Danio rerio) and guppy (Poecilia reticulata), which are considered the most commonly used model organisms in toxicity testing. The semi-static method according to OECD no. 203 (Fish, Acute toxicity test) was used for testing the toxicity of clove oil for juvenile fish. A series of 5 acute toxicity tests was performed, with 10 fish of both species used for each concentration and for the control. The results obtained (number of dead individuals at particular test concentrations) were subjected to a probit analysis using the EKO-TOX 5.2 program in order to determine 96hLC50 clove oil values. The significance of the difference between 96hLC50 values in D. rerio and P. reticulata was tested using the Mann-Whitney non-parametric test. The 96hLC50 mean value for clove oil was 18.2 ± 5.52 mg·l–1 in juvenile D. rerio and 21.7 ± 0.8 mg·l–1 in P. reticulata. In spite of variability in clove oil composition, acute toxicity values of clove oil for juvenile stages of both fish species were comparable. The results did not show different sensitivities to clove oil in tested fish species. This is the first similar study in these fish species.

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