Use of response surface methodology to study the combined effects of temperature and salinity on hatching and deformity of the hybrid grouper, Epinephelus fuscoguttatus (♀) × Epinephelus polyphekadion (♂)

2018 ◽  
Vol 49 (5) ◽  
pp. 1997-2005 ◽  
Author(s):  
Jiansheng Huang ◽  
Gang Chen ◽  
Zhongliang Wang ◽  
Jiandong Zhang
2019 ◽  
Vol 09 ◽  
Author(s):  
Hossein Zaeri ◽  
Bahareh Kamyab Moghadas ◽  
Bijan Honarvar ◽  
Ali Shokuhi Rad

: In this research, the extraction of essential oil from Calotropis Procera with the family name of Asclepiadaceae, by supercritical carbon dioxide (CO2) solvent has been investigated in detail, and the yield and chemical profile of the extracts achieved by this method were compared with those resulted by the conventional Hydro distillation method. To optimize the process parameters of CO2 supercritical extraction (SCE) of the Calotropis Procera, the Response Surface Methodology (RSM) with central composite design (CCD) was employed. The effects of temperature, pressure, and extraction time on the oil yield are considered for investigation. Results showed that the data were sufficiently fitted into the second-order polynomial model. The extraction conditions, including pressure, temperature, and extraction time, were studied between 150-200 bar, 40-50 ºC, and 50-100 min, respectively. The optimal conditions are achieved as the temperature of 47.19ºC, the pressure of 172.2 bar, and time of 86 minutes with the retrieval rate of 31.39%.


Toxins ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 499
Author(s):  
Xiaofang Lu ◽  
Chaoquan Luo ◽  
Jianyong Xing ◽  
Zhengzhou Han ◽  
Tong Li ◽  
...  

The root of Ilex asprella is a commonly used herb in Southern China, and also constitutes the main raw material of Canton herbal tea. I. asprella is readily contaminated by mildew because of rich nutrients. Aspergillus versicolor producing sterigmatocystin is one of the most common molds that contaminate foodstuffs and medicinal herbs. Previous study on the evaluation of fungal contamination on samples of I. asprella revealed that A. versicolor was the dominant contaminant. In this study, experiments based on response surface methodology combined with central composite design were carried out to determine the optimal storage conditions of I. asprella to minimize the contamination of sterigmatocystin. The herb, manually innoculated with A. versicolor, was stored under different temperatures (20–40 °C) and humidity (80–95%) for seven days. The effects of temperature and humidity were evaluated using total saponin, polysaccharide and the sterigmatocystin levels as quality indexes. The results showed that A. versicolor grew quickly and produced large amounts of sterigmatocystin on I. asprella, at humidity ranging from 85% to 90% and temperatures above 26 °C. Meanwhile, total saponin and polysaccharide amounts were reduced significantly. These findings suggested that I. asprella samples should be stored in an environment with humidity and temperature below 85% and 26 °C, respectively, to reduce A. versicolor growth and sterigmatocystin production.


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