Molecular cloning of heat shock protein 60 from Marsupenaeus japonicus and its expression profiles at early developmental stages and response to heat stress

2017 ◽  
Vol 49 (1) ◽  
pp. 301-312 ◽  
Author(s):  
Jinbin Zheng ◽  
Lijun Li ◽  
Hongbiao Dong ◽  
Yong Mao ◽  
Yongquan Su ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Molecular Cloning ◽  
Developmental Stages ◽  
Expression Profiles ◽  
Heat Shock Protein 60 ◽  
Marsupenaeus Japonicus ◽  
Early Developmental Stages ◽  
Early Developmental

Molecular cloning, sequence analysis and stress-related changes of the heat shock protein 60 gene in Neobenedenia melleni

2013 ◽  
Vol 33 (6) ◽  
pp. 603-608 ◽  
Author(s):  
Fang WANG ◽  
Jiong CHEN ◽  
Yu-Hong SHI ◽  
Xin-Jiang LU ◽  
Ming-Yun LI
Keyword(s):  
Sequence Analysis ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Molecular Cloning ◽  
Heat Shock Protein 60

scholarly journals Heat shock protein (HSP) expression and mitogen-activated protein kinase (MAPK) phosphorylation during early embryonic developmental stages of the Gilthead sea bream (Sparus aurata)

2018 ◽  
pp. 240 ◽  
Author(s):  
CHRYSOULA ROUFIDOU ◽  
KONSTANTINOS FEIDANTSIS ◽  
ELENI MENTE ◽  
ELENA SARROPOULOU ◽  
EFTHIMIA ANTONOPOULOU
Keyword(s):  
Heat Shock ◽  
Protein Expression ◽  
P38 Mapk ◽  
Developmental Stages ◽  
Sparus Aurata ◽  
Sea Bream ◽  
Mapk Phosphorylation ◽  
Early Developmental Stages ◽  
Mitogen Activated Protein ◽  
Early Developmental

Both heat shock proteins (HSPs), which have key roles in vital cell functions, as well as members of the mitogen-activated protein kinases (MAPKs), which adjust gene expression by transducing cellular signals to the nucleus, are necessary for normal embryonic development in vertebrates. Therefore, protein expression levels of HSP70 and HSP90 and the activation of members of the MAPK protein family, such as p38 MAPK, ERKs, and JNKs were studied in the early developmental stages of the Gilthead sea bream, Sparus aurata Linnaeus, 1758. The protein expression of HSP70 and the phosphorylation ratio of JNKs remained at equal levels at all examined developmental stages, while the other examined proteins exhibited a differential profile. HSP90 levels were mostly increased at the 16-cell stage and towards the morula stages, and the lowest values were observed at the two- to four-cell and one-half epiboly stages. While p38 MAPK phosphorylation ratio exhibited increased values mostly in the early developmental stages, the opposite was observed concerning ERK phosphorylation ratio, where increased values were observed in the later embryonic stages (high blastula to one-half epiboly stages). These differential profiles of the examined protein expression levels highlight the importance of these proteins during embryogenesis and pave the way for further research to unveil their distinct role in early development.

scholarly journals New Insights into Evolution of Plant Heat Shock Factors (Hsfs) and Expression Analysis of Tea Genes in Response to Abiotic Stresses

Plants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 311
Author(s):  
Ping Xu ◽  
Qinwei Guo ◽  
Xin Pang ◽  
Peng Zhang ◽  
Dejuan Kong ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Plant Species ◽  
Abiotic Stresses ◽  
Developmental Stages ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Heat Shock Factors ◽  
Camellia Species ◽  
Species Specific

Heat shock transcription factor (Hsf) is one of key regulators in plant abotic stress response. Although the Hsf gene family has been identified from several plant species, original and evolution relationship have been fragmented. In addition, tea, an important crop, genome sequences have been completed and function of the Hsf family genes in response to abiotic stresses was not illuminated. In this study, a total of 4208 Hsf proteins were identified within 163 plant species from green algae (Gonium pectorale) to angiosperm (monocots and dicots), which were distributed unevenly into each of plant species tested. The result indicated that Hsf originated during the early evolutionary history of chlorophytae algae and genome-wide genetic varies had occurred during the course of evolution in plant species. Phylogenetic classification of Hsf genes from the representative nine plant species into ten subfamilies, each of which contained members from different plant species, imply that gene duplication had occurred during the course of evolution. In addition, based on RNA-seq data, the member of the Hsfs showed different expression levels in the different organs and at the different developmental stages in tea. Expression patterns also showed clear differences among Camellia species, indicating that regulation of Hsf genes expression varied between organs in a species-specific manner. Furthermore, expression of most Hsfs in response to drought, cold and salt stresses, imply a possible positive regulatory role under abiotic stresses. Expression profiles of nineteen Hsf genes in response to heat stress were also analyzed by quantitative real-time RT-PCR. Several stress-responsive Hsf genes were highly regulated by heat stress treatment. In conclusion, these results lay a solid foundation for us to elucidate the evolutionary origin of plant Hsfs and Hsf functions in tea response to abiotic stresses in the future.

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