Effects of salinity on osmoregulation during the embryonic development of the bullseye puffer (Sphoeroides annulatusJenyns 1842)

Javier Pérez-Robles; Fernando Diaz; Leonardo Ibarra-Castro; Ivone Giffard-Mena; Ana Denise Re; Luz Estela Rodríguez Ibarra; Jesús Armando Ibarra Soto

doi:10.1111/are.12542

Effects of salinity on osmoregulation during the embryonic development of the bullseye puffer (Sphoeroides annulatusJenyns 1842)

Aquaculture Research ◽

10.1111/are.12542 ◽

2014 ◽

Vol 47 (3) ◽

pp. 838-846 ◽

Cited By ~ 6

Author(s):

Javier Pérez-Robles ◽

Fernando Diaz ◽

Leonardo Ibarra-Castro ◽

Ivone Giffard-Mena ◽

Ana Denise Re ◽

...

Keyword(s):

Embryonic Development ◽

Bullseye Puffer

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Live Confocal Analysis of Fertilization and Early Development

Microscopy and Microanalysis ◽

10.1017/s1431927600031135 ◽

2001 ◽

Vol 7 (S2) ◽

pp. 1012-1013

Author(s):

Uyen Tram ◽

William Sullivan

Keyword(s):

Drosophila Melanogaster ◽

Embryonic Development ◽

Real Time ◽

Early Development ◽

Fluorescent Probes ◽

Primary Defect ◽

Fluorescent Analysis ◽

Dynamic Event ◽

Enormous Amount ◽

Fluorescent Studies

Embryonic development is a dynamic event and is best studied in live animals in real time. Much of our knowledge of the early events of embryogenesis, however, comes from immunofluourescent analysis of fixed embryos. While these studies provide an enormous amount of information about the organization of different structures during development, they can give only a static glimpse of a very dynamic event. More recently real-time fluorescent studies of living embryos have become much more routine and have given new insights to how different structures and organelles (chromosomes, centrosomes, cytoskeleton, etc.) are coordinately regulated. This is in large part due to the development of commercially available fluorescent probes, GFP technology, and newly developed sensitive fluorescent microscopes. For example, live confocal fluorescent analysis proved essential in determining the primary defect in mutations that disrupt early nuclear divisions in Drosophila melanogaster. For organisms in which GPF transgenics is not available, fluorescent probes that label DNA, microtubules, and actin are available for microinjection.

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