Acidified seawater suppresses insulin-like growth factor I mRNA expression and reduces growth rate of juvenile orange-spotted groupers,Epinephelus coioides(Hamilton, 1822)

2014 ◽  
Vol 47 (3) ◽  
pp. 721-731 ◽  
Author(s):  
Yi Ta Shao ◽  
Feng Yu Chang ◽  
Wen-Chun Fu ◽  
Hong Young Yan
Keyword(s):  
Growth Rate ◽  
Growth Factor ◽  
Mrna Expression ◽  
Epinephelus Coioides ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Growth Factor I

Hormonal control of insulin-like growth factor-I and growth hormone receptor mRNA expression by porcine hepatocytes in culture

1995 ◽  
Vol 146 (2) ◽  
pp. 239-245 ◽  
Author(s):  
J M Brameld ◽  
P A Weller ◽  
J C Saunders ◽  
P J Buttery ◽  
R S Gilmour
Keyword(s):  
Growth Factor ◽  
Thyroid Hormones ◽  
Mrna Expression ◽  
Culture Medium ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Igf I ◽  
Class 1 ◽  
Growth Factor I ◽  
Porcine Hepatocytes

Abstract The effects of various hormones commonly added to hepatocyte culture media upon the expression of the GH receptor (GHR) and insulin-like growth factor-I (IGF-I) genes in cultured porcine hepatocytes were investigated. Preliminary investigations indicated that there was an absolute requirement only for insulin, with high losses of cell viability upon long term exclusion of insulin from the culture medium. The decline in GHR expression with time in culture was found to be less when high levels of glucose were included in the medium. Therefore the basal culture medium used in these studies was Williams' medium E supplemented with 0·2% (w/v) BSA, 5000 mg glucose/l and 100 nmol porcine insulin/l. The addition of dexamethasone (100 nmol/l) increased the expression of both GHR and IGF-I (class 1 transcripts only) mRNA (P<0·001 and P<0·05 respectively), and resulted in an increased responsiveness of IGF-I mRNA expression to GH (1 μg/ml), when the two were added in combination (although only class 1 transcripts were shown to be statistically significant, P<0·01). The addition of either thyroid hormone (1 nmol/l T3 or T4) alone also increased the expression of GHR mRNA (P<0·01) in addition to the dexamethasone stimulated expression, with T4 appearing to decrease IGF-I expression slightly (P<0·05) (either on its own or with T3). As with dexamethasone, the thyroid hormones increased the response of IGF-I mRNA expression to GH (1 μg/ml) when added in combination with GH (P<0·001). These observations demonstrate one possible mechanism for the interactions of glucocorticoids and thyroid hormones with the GH–IGF axis. Journal of Endocrinology (1995) 146, 239–245

scholarly journals The effect of hypophysectomy on pancreatic islet hormone and insulin-like growth factor I content and mRNA expression in rat

2005 ◽  
Vol 123 (2) ◽  
pp. 179-188 ◽  
Author(s):  
Tanja Jevdjovic ◽  
Caroline Maake ◽  
Cornelia Zwimpfer ◽  
Gunthild Krey ◽  
Elisabeth Eppler ◽  
...  
Keyword(s):  
Growth Factor ◽  
Mrna Expression ◽  
Pancreatic Islet ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Islet Hormone ◽  
Growth Factor I
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