In vitroeffect of low salinity on egg hatching and larval survival ofHeterobothrium ecuadori(Monogenea) infecting bullseye puffer fishSphoeroides annulatus

2013 ◽  
Vol 46 (6) ◽  
pp. 1522-1526 ◽  
Author(s):  
Mayra I. Grano-Maldonado ◽  
Hugo Aguirre-Villaseñor ◽  
Miguel Betancourt-Lozano ◽  
Emma J. Fajer-Ávila
Keyword(s):  
Larval Survival ◽  
Egg Hatching ◽  
Low Salinity ◽  
Bullseye Puffer

scholarly journals Daya Tetas Telur dan Sintasan Larva Dari Hasil Penambahan Madu pada Bahan Pengencer Sperma Ikan Nila (Oreochromis niloticus)

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Yunus Ayer ◽  
Joppy Mudeng ◽  
Hengky Sinjal
Keyword(s):  
Experimental Design ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Larval Survival ◽  
Hatching Rate ◽  
Dilution Ratio ◽  
Egg Hatching ◽  
Research Results ◽  
Spermatozoa Motility ◽  
Randomized Design

The objective of research was to determine the concentration of honey in sperm dilution  for improving hatching rate of egg and survival of nile tilapia larvae (Oreochromis niloticus).The number of fish used was five pairs broodstock. Dilution substance was NaCl and honey.  Observations were conducted on spermatozoa motility, fertility and egg hatching rate. Experimental design used was complete randomized design. Sperm dilution substance was prepare by disolving honey  (0 mL; 0,60 mL; 0,65 mL dan 0,70 mL) in 100 mL; 99,40 mL; 99,35 mL and  99,30 mL NaCl respectively.  Each dilution was homogenized using aerator for 15 minutes. Fertilization was done 12 hours after ovulation.  Egg hatching rate was observed after ovulation. Research results showed the use of honey had significant effet on egg hatching rate, but not on larval survival.  Treatment D (0,70 mL honey in 99,30 mL NaCl) had the highest hatching rate (77.33%). Dilution ratio 1:60 was the best indicated by spermatozoa motility 96.66%, fertility 71.65, hatching rate 70% and larval survival 81.67%   Keywords : honey, egg hatching rate, larval survival, Oroechromnis niloticus

scholarly journals Influences of Temperature on Development and Survival, Reproduction and Growth of a Calanoid Copepod (Pseudodiaptomus dubia)

2009 ◽  
Vol 9 ◽  
pp. 866-879 ◽  
Author(s):  
Changling Li ◽  
Xiaoxia Luo ◽  
Xianghu Huang ◽  
Binhe Gu
Keyword(s):  
Growth Rate ◽  
Water Temperature ◽  
Larval Development ◽  
Calanoid Copepod ◽  
Southern China ◽  
Larval Survival ◽  
Population Doubling ◽  
Population Doubling Time ◽  
Reproduction Rate ◽  
Egg Hatching

Pseudodiaptomus dubiais a calanoid copepod that is distributed widely in the estuarine-coastal waters of Asia and is a dominant copepod in the shrimp grow-out ponds in southern China. A laboratory culture experiment was conducted to evaluate the influences of water temperature on larval development, survival, and reproduction. Results indicate that within a temperature range from 15 to 35°C, larval development increases as the temperature increases. The water temperature for optimal larval survival rate ranges from 20 to 35°C. Longevity and egg hatching time decrease as the temperature increases from 20 to 35°C. Total fecundity and reproduction frequency increase as the water temperature increases, with the maximum at 30°C. Fecundity and reproduction frequency decrease when the temperature exceeds 30°C. Intrinsic growth rate (rm) ranges from 0.168 to 0.195 at 25 to 30°C; net reproduction rate (R0) and finite growth rate (?) are 163 to 264 and 1.183 to 1.215, respectively, when the temperature is greater than 20 and 35°C; population doubling time (t) varies from 3.556 to 4.128 days at temperatures less than 20 and 35°C. Population generation time (T) is negatively correlated with temperature, with the optimal population growth rate at 25 to 30°C.

scholarly journals The effect of amino acid analogues on larval growth and survival of the olive fruit fly Bαctrocerα oleαe (Diptera: Tephritidae)

2017 ◽  
Vol 13 ◽  
pp. 23
Author(s):  
Α. G. Manoukas
Keyword(s):  
Amino Acid ◽  
Larval Growth ◽  
Adult Emergence ◽  
Fruit Fly ◽  
Larval Survival ◽  
Pupal Weight ◽  
Olive Fruit Fly ◽  
Egg Hatching ◽  
Olive Fruit ◽  
Amino Acid Analogues

The effects of eight amino acid analogues [L-canavanine, D-cycloserine, allylglycine, L-glutamic acid-A-hydrazide, DL-ethionine, L-,-3,4 dihydroxyphenylalanine (L-DOPA), DL-,-3,4 dihydroxyphenyl-alanine (DL-DOPA) and thiaproline] added to an artificial diet on egg hatching, larval survival, larval weight, pupal weight and adult emergence of the olive fruit fly, Bactrocera oleae (Gmelin) (Diptera: Tephritidae) were investigated. Larval survival and weight were significantly decreased by all amino acid analogues tested. Pupal weight and adult emergence was depressed by L-canavanine, D-cycloserine, L-DOPA, DL-DOPA and allyl-glycine. Of all amino acid analogues tested only L-canavanine inhibited hatching of the eggs. The depression of the parameters affected was increased by increasing the concentration of each analogue tested. The larvae of most experimental diets took longer to pupate than those of the control.

scholarly journals PATOGENISITAS CENDAWAN Lecanicillium sp. PTN01 TERHADAP PENGGEREK TONGKOL JAGUNG Helicoverpa armigera (HUBNER) (LEPIDOPTERA: NOCTUIDAE)

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Sempurna Ginting ◽  
Teguh Santoso ◽  
Yayi Munara ◽  
Ruly Anwar ◽  
Lisdar Sudirman
Keyword(s):  
Helicoverpa Armigera ◽  
Larval Mortality ◽  
Larval Survival ◽  
Reproductive Rate ◽  
Economic Losses ◽  
Control Group ◽  
Pathogenicity Test ◽  
Agricultural Pests ◽  
Egg Hatching ◽  
Helicoverpa Armígera

Helicoverpa armigera is one of the most important agricultural pests because it has a high reproductive rate and resulted in economic losses. One of the H. armigera control techniques that is in accord with IPM principle is the utilizing of entomopathogenic fungus. The aim of this research was to test the pathogenicity of Lecanicillium (Lecanicillium sp. PTN01) against H. armigera. Pathogenicity test was conducted by conidia application of Lecanicillium sp. PTN01 on both the larvae and eggs at density of 105, 106, 107 conidia/ml. The control group was only treated with steril water. Polymerase chain reaction (PCR) was performed for molecular identification Lecanicillium sp. PTN01. The results showed that Lecanicillium sp. PTN01 able to inhibit egg hatching 13.75%, and cause mortality of first instar larval survival 98.75%. The result of virulence test to the highest larval mortality at 107 conidia/ml density was 41,25%, with the values of LT25, 50, 75 were 3.95, 7.12, 12.82 (days) and LC25, 50, 75 were 4.6 x 105, 1.7 x 106, 4.6 x 109 (conidia/ml). The DNA sequence analysis of ITS 1 and ITS 4 primers showed that Lecanicillium sp. PTN01 was similar to fungus species L. kalimantanense strain BTCC F23 with 94% homology.   

Effect of temperature on egg hatching and survival of larvae of Anisakis simplex B (Nematoda: Ascaridoidea)

1992 ◽  
Vol 70 (2) ◽  
pp. 274-279 ◽  
Author(s):  
John Brattey ◽  
Kirsten J. Clark
Keyword(s):  
Larval Survival ◽  
Anisakis Simplex ◽  
Effect Of Temperature ◽  
Curvilinear Relationship ◽  
Survival Times ◽  
Egg Hatching ◽  
Hatching Time ◽  
Length Width ◽  
Wet Weight ◽  
Newly Hatched Larvae

Eggs of the parasitic nematode Anisakis simplex B, obtained from a white-beaked dolphin (Lagenorhynchus albirostris Gray), were incubated in seawater at constant temperatures from −0.7 to 24.3 °C and hatching times determined. Eggs hatched at all temperatures except −0.7 °C. The number of days until hatching ranged from 3 at 24.3 °C to 74–81 at 1.9 °C. There was a declining curvilinear relationship between temperature and both hatching time and duration of the hatching period. The hatching period increased and became more variable with decreasing temperature and ranged from 5 days at 24.3 °C to 55–72 days at 3.8 °C. The percentage of eggs that hatched at each temperature was variable but generally fell in the range 30–60% and was not correlated with temperature. Survival times of hatched larvae peaked at 75–105 days at 8.6 °C, with a minimum of 3–8 days at 24.3 °C. Dimensions of eggs (length, width, volume) differed significantly among individual worms but were not correlated with worm size (wet weight). Egg hatching and larval survival characteristics indicate a species adapted to cold-temperate waters and are consistent with the known distribution of A. simplex B. Preliminary experiments indicated that harpacticoid copepods will ingest newly hatched larvae of A. simplex B and become infected, suggesting that these microcrustaceans may be involved in the life cycle of the nematode.

Effects of adult salinity acclimation on larval survival and early development of Strongylocentrotus droebachiensis and Strongylocentrotus pallidus (Echinodermata: Echinoidea)

1994 ◽  
Vol 72 (11) ◽  
pp. 1931-1939 ◽  
Author(s):  
Richard A. Roller ◽  
William B. Stickle
Keyword(s):  
Sea Water ◽  
Sea Urchins ◽  
Subsequent Development ◽  
Larval Survival ◽  
Strongylocentrotus Droebachiensis ◽  
Salinity Acclimation ◽  
Percent Survival ◽  
Low Salinity ◽  
Development Rates ◽  
Survival And Development

Larval survival and development rates of Strongylocentrotus droebachiensis and Strongylocentrotus pallidus were determined as a function of salinity in two experiments by (i) directly transferring fertilized eggs obtained from adults acclimated to sea water at a salinity of 30‰ to cultures containing seawater at salinities of 30, 27.5, 25, 22.5, 20, 17.5, 15, 12.5, and 10‰ at 10 °C; and (ii) acclimation of adult sea urchins to the salinity–temperature conditions described above for 2, 3, and 4 weeks prior to spawning. Subsequent development occurred under these acclimation conditions. Development rates and percent survival of larvae prior to metamorphosis varied directly with salinity. Survival of S. pallidus plutei to metamorphosis decreased at salinities below 30‰. Strongylocentrotus droebachiensis plutei survived to metamorphosis at 20‰ and above. Lactic acid concentrations in the coelomic cavity fluid of adult S. droebachiensis and S. pallidus acclimated to low salinity were significantly higher than initial controls at 30‰. Cell volumes of fertilized eggs of both species exhibited osmotic swelling when exposed to lowered salinity. LC50 values (‰), development rates, and percent survival to metamorphosis indicate that acclimation of adult urchins to lower salinity prior to spawning and fertilization does not enhance development or survival of embryos of these two species exposed to low salinity. Furthermore, our results show that S. pallidus larvae are stenohaline when compared with larvae of other echinoderm species.

scholarly journals A comparative study on the effect of dispersed and undispersed Kuwait crude oil on egg hatching and larval survival of Epinephelus coioides

2019 ◽  
Vol 40 (2) ◽  
pp. 192-199 ◽  
Author(s):  
Q. Karam ◽  
◽  
M. Ali ◽  
M.N.V. Subrahmanyam ◽  
K. Al-Abdul Elah ◽  
...  
Keyword(s):  
Comparative Study ◽  
Crude Oil ◽  
Larval Survival ◽  
Epinephelus Coioides ◽  
Egg Hatching

Effects of soil conditions and drought on egg hatching and larval survival of the clover root weevil (Sitona lepidus)

2010 ◽  
Vol 44 (1) ◽  
pp. 75-79 ◽  
Author(s):  
Scott N. Johnson ◽  
Peter J. Gregory ◽  
James W. McNicol ◽  
Yasmina Oodally ◽  
Xiaoxian Zhang ◽  
...  
Keyword(s):  
Larval Survival ◽  
Soil Conditions ◽  
Egg Hatching ◽  
Sitona Lepidus ◽  
Clover Root

Effects of elevated hydrogen ion and aluminum concentrations on the survival of amphibian embryos and larvae

1985 ◽  
Vol 63 (1) ◽  
pp. 116-123 ◽  
Author(s):  
Karen L. Clark ◽  
Ronald J. Hall
Keyword(s):  
Hatching Success ◽  
Rana Sylvatica ◽  
Larval Survival ◽  
Hydrogen Ion ◽  
Ph Effects ◽  
Bufo Americanus ◽  
Amphibian Species ◽  
Egg Hatching ◽  
Effects Of Ph ◽  
Chemical Variable

Egg hatching success was measured for three amphibian species (Bufo americanus, Rana sylvatica, and Ambystoma maculatum) exposed to varying hydrogen ion, aluminum, and dissolved organic carbon (DOC) concentrations. In the first experiment, hatching success in six stream pools was negatively correlated with Al and DOC and positively correlated with pH. Effects of any one chemical variable, however, could not be separated from the others. In a second experiment, to separate effects of pH and Al from DOC, eggs and larvae were exposed to simulated stream pools in which pH was reduced. At pH 4.8 and 37 μg/L inorganic monomeric Al, only eggs of A. maculatum were affected. At pH 4.3, 46 μg/L inorganic monomeric Al was more toxic (compared with 34–35 μg/L at pH 4.8 and 4.3) to B. americanus and R. sylvatica embryos. Bufo americanus was more sensitive to low pH and elevated Al than R. sylvatica. Mortality of eggs of both species mainly occurred within 1 day of exposure but continued until hatching. Mortality of A. maculatum eggs, however, did not occur during the 4 days of acidification, but 2–3 weeks later, at the time of hatching. Larval survival in general was not affected by reduced pH (pH 4.3) and (or) elevated Al (up to 46 μg/L inorganic monomeric Al).

scholarly journals Induced spawning of Asian bumblebee catfish (Pseudomystus siamensis Regan, 1913)

2020 ◽  
Vol 19 (01) ◽  
pp. 49-58
Author(s):  
Binh T. T. Vo
Keyword(s):  
Luteinizing Hormone ◽  
Oocyte Maturation ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Artificial Insemination ◽  
Larval Survival ◽  
Hormone Analogue ◽  
Egg Hatching ◽  
Luteinizing Hormone Releasing Hormone ◽  
Final Oocyte Maturation

Asian bumblebee catfish (Pseudomystus siamensis Regan, 1913) is an indigenous and economically valuable species in southern provinces of Vietnam. It is being exploited for food and ornamentation. This study used fish pituitary gland (FPG), human chorionic gonadotropin (HCG) and luteinizing hormone releasing hormone analogue (LHRHa) to induce spawning of the fish. The three spawning inducers affected on final oocyte maturation (FOM) and ovulation (FOMO) of females. The optimal revolving dose to induce FOM and FOMO was 10 mg/kg for FPG, 4.000 UI/kg for HCG and 120 μg/kg of female for LHRHa. The suitable periods of egg stripping and artificial insemination were from 9 - 11 h post resolving injection (hpri). Maximum spawning response was obtained with the optimal revolving dose of LHRHa (120 μg/kg). At the suitable stripping period (10 hpri) the rates of spawning, egg fertilization, egg hatching, larval survival, and relative fecundity were 64.5%, 70%, 44.5%, 39.3%, and 38,500 egg/kg of female, respectively.

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