scholarly journals Erythropoietin‐producing cells in the kidney: Novel insights in their long‐term fate during hypoxaemia and renal tissue remodeling

2022 ◽  
Author(s):  
Holger Scholz
Keyword(s):  
Tissue Remodeling ◽  
Renal Tissue

Stromal fibroblasts synthesize collagenase and stromelysin during long-term tissue remodeling

1993 ◽  
Vol 104 (4) ◽  
pp. 1001-1011
Author(s):  
M.T. Girard ◽  
M. Matsubara ◽  
C. Kublin ◽  
M.J. Tessier ◽  
C. Cintron ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Tissue Remodeling ◽  
Enzyme Synthesis ◽  
Stromal Fibroblasts ◽  
Repair Tissue ◽  
New Synthesis ◽  
Direct Support ◽  
Connective Tissue Remodeling ◽  
Specific Mrna

The process of connective tissue remodeling is an important mechanism contributing to tissue morphogenesis in development and homeostasis. Although it has long been known that remodeling tissues actively mediate collagenolysis, little is understood about the molecular mechanisms controlling this cell-regulated process. In this study, we examined the biosynthesis of collagenase and the related metalloproteinase, stromelysin, during remodeling of repair tissue deposited after mechanical injury to the rabbit cornea. Neither enzyme was synthesized by uninjured corneas; however, synthesis and secretion was detectable within one day after injury. Collagenase accumulated in its latent form while stromelysin appeared to be partially activated. Enzymes were synthesized by cells having a fibroblast phenotype. These cells were found within the stroma. New synthesis was correlated with accumulation of enzyme-specific mRNA. Highest levels of enzyme synthesis were observed in the repair tissue. However, stromal cells outside of the repairing area also synthesized both enzymes. The level of synthesis decreased in a gradient radiating from the repair tissue. Total synthetic levels in a given area of cornea were dependent on both the number of cells expressing enzyme and the rate of enzyme synthesis. Synthesis of collagenase was detected in repair tissue as long as nine months after injury. Our findings provide direct support for the hypothesis that new collagenase synthesis by cells in repair tissue is the first step in collagen degradation during long-term tissue remodeling.

scholarly journals Long-Term Exposure of Lead Acetate on Rabbit Renal Tissue

2016 ◽  
Vol 18 (2) ◽  
Author(s):  
Mohammad Hassan Karimfar ◽  
Afshar Bargahi ◽  
Darab Moshtaghi ◽  
Parviz Farzadinia
Keyword(s):  
Lead Acetate ◽  
Renal Tissue

Factors Contributing to Peritoneal Tissue Remodeling in Peritoneal Dialysis

2009 ◽  
Vol 29 (6) ◽  
pp. 605-617 ◽  
Author(s):  
Margot N. Schilte ◽  
Johanna W.A.M Celie ◽  
Piet M. ter Wee ◽  
Robert H.J. Beelen ◽  
Jacob van den Born
Keyword(s):  
Peritoneal Dialysis ◽  
Structural Changes ◽  
Tissue Remodeling ◽  
Therapeutic Strategies ◽  
Molecular Pathways ◽  
Peritoneal Membrane ◽  
Volume Loading ◽  
Peritoneal Cells ◽  
Peritoneal Tissue

Peritoneal dialysis (PD) is associated with functional and structural changes of the peritoneal membrane. In this review we describe factors contributing to peritoneal tissue remodeling, including uremia, peritonitis, volume loading, the presence of a catheter, and the PD fluid itself. These factors initiate recruitment and activation of peritoneal cells such as macrophages and mast cells, as well as activation of peritoneal cells, including mesothelial cells, fibroblasts, and endothelial cells. We provide an overview of cytokines, growth factors, and other mediators involved in PD-associated changes. Activation of downstream pathways of cellular modulators can induce peritoneal tissue remodeling, leading to ultrafiltration loss. Identification of molecular pathways, cells, and cytokines involved in the development of angiogenesis, fibrosis, and membrane failure may lead to innovative therapeutic strategies that can protect the peritoneal membrane from the consequences of long-term PD.

scholarly journals Co-occurrence of IgA nephropathy and IgG4-Tubulointersitial nephritis effectively treated with tacrolimus: a case report

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Mi Tian ◽  
Junjun Luan ◽  
Congcong Jiao ◽  
Qing Chang ◽  
Jeffrey B. Kopp ◽  
...  
Keyword(s):  
Renal Function ◽  
Complete Remission ◽  
Maintenance Treatment ◽  
Renal Tissue ◽  
Serum Igg4 Level ◽  
Serum Igg4 ◽  
Term Maintenance ◽  
Igg4 Level

Abstract Background Cases of concurrent immunoglobulin A nephropathy (IgAN) and IgG4-related tubulointerstitial nephritis (IgG4-TIN) are rare and previous case reports have lacked important data. KDIGO suggests a treatment with systemic glucocorticoids in IgAN patients. Glucocorticoids are recommended as the first-line therapy for IgG4-TIN. The use of tacrolimus as a long-term maintenance treatment has not been described. We report the case of a man who developed IgAN and IgG4-TIN without abnormalities in extra-renal tissue, without renal function abnormalities or impairment as well, and was treated by tacrolimus as a long-term maintenance during 45 months follow-up. Case presentation A 56-year-old Chinese man first presented to our hospital with the chief complaint of foamy urine for 1 year and hematuria for 3 months, with a medical history of hypertension. Testing revealed a notable increase in serum IgG4 level without abnormalities in renal function or imaging, or in dysfunction other organs. Renal biopsy showed mesangial extracellular matrix proliferation, increased mesangial cell numbers and infiltration of plasma cells. Immunofluorescence showed mesangial positivity for IgA and C3. Immunohistochemistry staining showed widespread IgG4 and increased CD38 and CD138 expression. Electron microscopy showed immune complexes located on the tubular basement membrane. He was diagnosed with IgAN and IgG4-TIN. He received glucocorticoids, leflunomide and tacrolimus to induce remission. He was given tacrolimus as long-term maintenance treatment. When tacrolimus was temporarily withdrawn, proteinuria recurred. After resuming tacrolimus therapy, he again entered complete remission. After 45 months of therapy, he remains in complete remission and the serum IgG4 level is normal. Conclusions The finding of concurrent IgAN and IgG4-TIN without abnormalities in renal function, imaging or extra-renal tissue is rare and their coexistence may be coincidental. Long-term treatment with tacrolimus proved effective and he has remained in remission during 45 months follow-up.

BENIGN FAMILIAL HEMATURIA

PEDIATRICS ◽  
1969 ◽  
Vol 44 (4) ◽  
pp. 590-593
Author(s):  
Melvin I. Marks ◽  
Keith N. Drummond
Keyword(s):  
Renal Disease ◽  
Family Members ◽  
Renal Tissue ◽  
Interstitial Tissue ◽  
History Of ◽  
Health And Development ◽  
Long Term Prognosis ◽  
General Physical ◽  
Hereditary Renal Disease

Asymptomatic persistent renal hematuria of 6 months to 8 years' duration was observed in seven of eight siblings. There was no family history of renal disease or deafness, and other family members examined showed normal urinalyses; none of the other known forms of hereditary renal disease were detected. Abnormalities of renal function or general physical health and development were not detected. Renal biopsies were performed in the two patients with the longest history of hematuria (7 and 8 years). Light and immunofluorescence microscopic study of renal tissue revealed no abnormalities of glomeruhi or interstitial tissue. This familial condition is characterized by persistent microscopic hematuria with episodic gross hematuria and a favorable long-term prognosis. Although the pathogenesis is unclear, an immune process does not appear to be involved. Microscopic examination of the urine of family members in hematuria without an obvious etiology is warranted.

Refraction Introscopy at Synchronized Radiation: Expansion of Possibilities for Visualization of Bone Tissue Remodeling

2010 ◽  
Vol 17 (2) ◽  
pp. 78-82
Author(s):  
D K Pogorelyi ◽  
A N Torgashin ◽  
K M Podurets ◽  
S S Rodionova
Keyword(s):  
Bone Tissue ◽  
Bone Remodeling ◽  
Tissue Remodeling ◽  
Laboratory Animals ◽  
Experimental Animals ◽  
Rat Tibia ◽  
Bone Structures ◽  
Bone Tissue Remodeling

Method of refraction introscopy at synchronized radiation for visualization of bone structures and allografts in laboratory animals was described. The aim of study was adaptation of method for the study of alloigrafts in bones of laboratory anomals. Method was approved at 150 samples of rat tibia. It was shown that use of refraction introscopy at synchronized radiation enabled properly to visualize the borders of allografts and own bone tissue of rat. Method may be recommended for the study of bone remodeling dynamics in experimental animals during long term after operation.

scholarly journals Autoimmune responses against renal tissue proteins in long-term surviving allograft recipients

2009 ◽  
Vol 22 (11) ◽  
pp. 1091-1099
Author(s):  
Cheng Fang ◽  
Caroline Ballet ◽  
Anne-Sophie Dugast ◽  
Anne Godard ◽  
Anne Moreau ◽  
...  
Keyword(s):  
Renal Tissue

scholarly journals NO-ERGIC SYSTEM IN RENAL TISSUE DURING BURN DISEASE

2019 ◽  
Vol 19 (2) ◽  
pp. 107-109
Author(s):  
Ya.O. Basarab
Keyword(s):  
Histological Study ◽  
Renal Tissue ◽  
Hot Water ◽  
Male Rats ◽  
Burn Shock ◽  
The Status ◽  
Inflammatory Processes ◽  
Series Of Experiments ◽  
Burn Disease

This research aimed at studying the state of the NO-ergic system in renal tissue of rats in modelled burn disease. Materials and methods. The series of experiments were carried out on pubescent male rats. Burn disease was modeled according to the Dovgansky method: the shaved and depilated area of a hind limb of test animals was dipped into hot water (t = 70 – 75 0С) under ethereal anesthesia for 7 sec. The size of the site damaged was determined depending on the area of ​​the skin, which averaged 12-15% of the animal’s body surface. The area of the skin damaged was calculated using the special Kochetygov’s table. Histological study of the damaged skin showed that under the above conditions there was a burn of IIIA-B degree that, according to modern conceptions, is considered as a standard model of the development of burn disease in the experiment. The rats were decapitated under ethereal anesthesia in 1, 7, 14, 21 days that in accordance with the modern ideas is commensurate with shock phases, early and late toxemia, and septicotoxemia. To evaluate the status of NO-ergic system, we assessed the NO-synthase activity and the nitrites content in the renal tissues. Results. Depending on the stage of burn disease, the NO-ergic system changed its characteristics. On the 1st day, at the stage of burn shock, the activity of NOS in renal tissues increased to its maximal level, while the peak increase in the content of nitrites was observed on the 7th day, at the stage of early toxemia. Conclusions. Thus, during the course of long-term burn disease, there is an increase in the activity of the NO-ergic system in kidney of rats. This can be explained by the enhancement of inflammatory processes in the kidneys under burn disease and by NO2– accumulation of in the renal tissues.

scholarly journals Exosomes Derived from SIRT6 Transfected Urine-Derived Stem Cells Improves Renal Fibrosis

2021 ◽  
Author(s):  
Rui Wang
Keyword(s):  
Stem Cells ◽  
Renal Fibrosis ◽  
Renal Tissue ◽  
Obstructive Nephropathy ◽  
Nanoparticle Tracking Analysis ◽  
Gene Expressions ◽  
Transmission Electron ◽  
Relative Gene ◽  
E Cadherin

Purpose: The treatment of renal fibrosis caused by long-term obstructive nephropathy is limited. The purpose of this study was to establish a mouse model of renal fibrosis with unilateral ureteral obstruction (UUO) and to treat it with exosomes derived from SIRT6 transfected urine-derived stem cells (USCs), which will determine whether exosomes have the effect of anti-fibrosis Methods: The renal fibrosis model of UUO mice was established by ligating unilateral ureter. USCs were extracted from human urine and transfected with SIRT6 lentivirus plasmid. The SIRT6-USCs-exosomes(SIRT6-USCs-exos) were collected and identified by transmission electron microscope, nanoparticle tracking analysis and western blot. SIRT6-USCs-exos were injected into the tail vein of mice and the renal tissue of mice was stained with HE. The relative gene expressions of α-SMA, E-cadherin and TGF-β1 were analyzed by RT-qPCR. Results: In this study, we successfully constructed the renal fibrosis model of UUO mice and hUSCs with overexpression of SIRT6. The SIRT6-USCs-exos significantly improved the renal fibrosis in UUO mice. The relative mRNA expressions of fibrosis-related genes α-SMA, E-cadherin and TGF-β1 in renal tissue were significantly down-regulated after SIRT6-USCs-exos treatment. Conclusion: Our results indicate that SIRT6-USCs-exos can effectively alleviate renal fibrosis caused by long-term obstructive nephropathy. The findings may be promising for dealing with renal fibrosis.

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