scholarly journals Physiological role of Kvβ2 (AKR6) in murine skeletal muscle growth and regulation

2018 ◽  
Vol 224 (2) ◽  
pp. e13083 ◽  
Author(s):  
K. C. Chapalamadugu ◽  
J. Tur ◽  
S. L. Badole ◽  
R. C. Kukreja ◽  
M. Brotto ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Growth ◽  
Physiological Role ◽  
Skeletal Muscle Growth ◽  
Murine Skeletal Muscle

scholarly journals The Possible Role of Complete Loss of Myostatin in Limiting Excessive Proliferation of Muscle Cells (C2C12) via Activation of MicroRNAs

2019 ◽  
Vol 20 (3) ◽  
pp. 643 ◽  
Author(s):  
Peixuan Huang ◽  
Daxin Pang ◽  
Kankan Wang ◽  
Aishi Xu ◽  
Chaogang Yao ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Muscle Growth ◽  
Interaction Network ◽  
Muscle Cells ◽  
Complete Loss ◽  
Skeletal Muscle Growth ◽  
Excessive Proliferation ◽  
Mouse Myoblast

Myostatin (MSTN) is a member of the TGF-β superfamily that negatively regulates skeletal muscle growth and differentiation. However, the mechanism by which complete MSTN deletion limits excessive proliferation of muscle cells remains unclear. In this study, we knocked out MSTN in mouse myoblast lines using a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) system and sequenced the mRNA and miRNA transcriptomes. The results show that complete loss of MSTN upregulates seven miRNAs targeting an interaction network composed of 28 downregulated genes, including TGFB1, FOS and RB1. These genes are closely associated with tumorigenesis and cell proliferation. Our study suggests that complete loss of MSTN may limit excessive cell proliferation via activation of miRNAs. These data will contribute to the treatment of rhabdomyosarcoma (RMS).

scholarly journals Effects of day-time feeding on murine skeletal muscle growth and synthesis

2019 ◽  
Vol 17 ◽  
pp. 100099 ◽  
Author(s):  
Shinya Aoyama ◽  
Shuichi Kojima ◽  
Keisuke Sasaki ◽  
Takeru Shimoda ◽  
Kengo Takahashi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Growth ◽  
Skeletal Muscle Growth ◽  
Murine Skeletal Muscle

scholarly journals 343 Wnt/β-catenin pathway is required for porcine satellite cell proliferation and differentiation to promote skeletal muscle growth

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 97-97
Author(s):  
Zong-ming Zhang ◽  
Chun-qi Gao ◽  
Hui-chao Yan ◽  
Xiu-qi Wang
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Satellite Cell ◽  
Muscle Growth ◽  
Control Group ◽  
Skeletal Muscle Growth ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Deficiency Group

Abstract Wnt/β-catenin plays a crucial role in skeletal muscle growth, but its specific mechanism still unclear. In this study, due to the distinct role of lysine in pig industry, we provided it as an entry point to investigate the role of Wnt/β-catenin in governing skeletal muscle growth. Firstly, total 18 weaned piglets were divided into three groups: control group, lysine deficiency group and lysine re-supplementation group (lysine levels added from 0.83% to 1.31% at 14 d). After 28 d experiment, all pigs were slaughtered to measure the change of Wnt/β-catenin in skeletal muscle. Secondly, satellite cell (SC) was isolated and cultured with Wnt activator, such as Wnt3a and WRN (Wnt3a, R-spondin1, Noggin) after lysine deficiency for 48 h to investigate cell proliferation and differentiation ability and the level of Wnt/β-catenin in different conditions. The results showed that compared with the control group, lysine deficiency significantly reduced longissimus dorsi muscle weight and Pax7 positive SC, and inhibited Wnt/β-catenin (P < 0.05). Fortunately, these restrictions were rescued to the control levels by lysine re-supplementation (P > 0.05). Meanwhile, compared with the lysine deficiency group, the MTT and western blotting assay showed cell proliferation ability was significantly increased with re-activated Wnt/β-catenin by re-supplemented lysine, Wnt3a or WRN (P < 0.05), respectively. Moreover, under the condition of cell differentiation, compared with the control group, cell fusion index was significantly decreased in the lysine deficiency group (P < 0.05), whereas it was significantly increased with lysine re-supplementation group, Wnt3a or WRN respective supplementation group in comparison with the lysine deficiency group (P < 0.05). In addition, compared with the lysine deficiency group, the protein levels of myogenic regulatory factors and Wnt/β-catenin pathway were also re-activated by re-supplemented lysine, Wnt3a or WRN (P < 0.05). Collectively, we found Wnt/β-catenin activation is required for porcine SC proliferation and differentiation to promote skeletal muscle growth.

Changes in collagen synthesis and degradation during skeletal muscle growth

1985 ◽  
Vol 249 (3) ◽  
pp. C352-C355 ◽  
Author(s):  
G. J. Laurent ◽  
R. J. McAnulty ◽  
J. Gibson
Keyword(s):  
Skeletal Muscle ◽  
Muscle Growth ◽  
Physiological Role ◽  
Synthesis Rate ◽  
Normal Muscle ◽  
Small Molecular Weight ◽  
Adult Chicken ◽  
Skeletal Muscle Growth ◽  
Anterior Latissimus Dorsi ◽  
Synthesis And Degradation

The changes in collagen metabolism during skeletal muscle growth were investigated by measuring rates of synthesis and degradation during stretch-induced hypertrophy of the anterior latissimus dorsi muscle of the adult chicken (Gallus domesticus). Synthesis rates were obtained from the uptake of tritiated proline injected intravenously with a flooding dose of unlabeled proline. Degradation of newly synthesized and "mature" collagen was estimated from the amount of hydroxyproline in the free pool as small molecular weight moieties. In normal muscle, the synthesis rate was 1.1 +/- 0.3%/day, with 49 +/- 7% of the newly produced collagen degraded rapidly after synthesis. During hypertrophy there was an increase of about fivefold in the rate of synthesis (P less than 0.01), a 60% decrease in the rate of degradation of newly synthesized collagen (P less than 0.02), and an increase of about fourfold in the amount of degradation of mature collagen (P less than 0.01). These results suggest an important role for degradative as well as synthetic processes in the regulation of collagen mass. They indicate that enhanced degradation of mature collagen is required for muscle growth and suggest a physiological role for the pathway whereby in normal muscle, a large proportion of newly produced collagen is rapidly degraded.

Chapter 5 Role of cytokines in skeletal muscle growth and differentiation

2002 ◽  
pp. 97-126
Author(s):  
B.B. Olwin ◽  
Y. Bren-Mattison ◽  
D.D.W. Cornelison ◽  
Y.V. Fedorov ◽  
H. Flanagan-Steet ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Growth ◽  
Skeletal Muscle Growth

scholarly journals Regulation of murine skeletal muscle growth by STAT5B is age- and sex-specific

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ryan G. Paul ◽  
Alex S. Hennebry ◽  
Marianne S. Elston ◽  
John V. Conaglen ◽  
Chris D. McMahon
Keyword(s):  
Skeletal Muscle ◽  
Muscle Growth ◽  
Skeletal Muscle Growth ◽  
Murine Skeletal Muscle ◽  
Age And Sex

Role of Satellite Cells in FoxO1-Mediated Skeletal Muscle Growth

2007 ◽  
Vol 39 (Supplement) ◽  
pp. S223
Author(s):  
Alissa DeLong ◽  
Yasutomi Kamei ◽  
Shinji Miura ◽  
Osamu Ezaki ◽  
Thomas J. McLoughlin
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cells ◽  
Muscle Growth ◽  
Skeletal Muscle Growth
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