Visceral adipose tissue-derived serine protease inhibitor augments acetylcholine-induced relaxation via the inhibition of acetylcholine esterase activity in rat isolated mesenteric artery

2015 ◽  
Vol 216 (2) ◽  
pp. 203-210 ◽  
Author(s):  
S. Kameshima ◽  
K. Yamada ◽  
T. Morita ◽  
M. Okada ◽  
H. Yamawaki
Keyword(s):  
Adipose Tissue ◽  
Protease Inhibitor ◽  
Serine Protease ◽  
Visceral Adipose Tissue ◽  
Esterase Activity ◽  
Mesenteric Artery ◽  
Serine Protease Inhibitor ◽  
Acetylcholine Esterase ◽  
Visceral Adipose

Monoamine oxidase is a source of oxidative stress in obese patients with chronic inflammation

2019 ◽  
Vol 97 (9) ◽  
pp. 844-849 ◽  
Author(s):  
Adrian Sturza ◽  
Sorin Olariu ◽  
Mihaela Ionică ◽  
Oana M. Duicu ◽  
Adrian O. Văduva ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adipose Tissue ◽  
Visceral Adipose Tissue ◽  
Mesenteric Artery ◽  
Vascular Reactivity ◽  
Ros Production ◽  
Obese Patients ◽  
Quantitative Real Time Pcr ◽  
Mao A ◽  
Visceral Adipose

Obesity is an important preventable risk factor for morbidity and mortality from cardiometabolic disease. Oxidative stress (including in visceral adipose tissue) and chronic low-grade inflammation are the major underlying pathomechanisms. Monoamine oxidase (MAO) has recently emerged as an important source of cardiovascular oxidative stress. The present study was conducted to evaluate the role of MAO as contributor to reactive oxygen species (ROS) production in white adipose tissue and vessels harvested from patients undergoing elective abdominal surgery. To this aim, visceral adipose tissue and mesenteric artery branches were isolated from obese patients with chronic inflammation and used for organ bath, ROS production, quantitative real-time PCR, and immunohistology studies. The human visceral adipose tissue and mesenteric artery branches contain mainly the MAO-A isoform, as shown by the quantitative real-time PCR and immunohistology experiments. A significant upregulation of MAO-A, the impairment in vascular reactivity, and increase in ROS production were found in obese vs. non-obese patients. Incubation of the adipose tissue samples and vascular rings with the MAO-A inhibitor (clorgyline, 30 min) improved vascular reactivity and decreased ROS generation. In conclusion, MAO-A is the predominant isoform in human abdominal adipose and vascular tissues, is overexpressed in the setting of inflammation, and contributes to the endothelial dysfunction.

scholarly journals Glyceroneogenesis is inhibited through HIV protease inhibitor-induced inflammation in human subcutaneous but not visceral adipose tissue

2010 ◽  
Vol 52 (2) ◽  
pp. 207-220 ◽  
Author(s):  
Stéphanie Leroyer ◽  
Camille Vatier ◽  
Sarah Kadiri ◽  
Joëlle Quette ◽  
Charles Chapron ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Protease Inhibitor ◽  
Visceral Adipose Tissue ◽  
Hiv Protease ◽  
Hiv Protease Inhibitor ◽  
Visceral Adipose

scholarly journals Vaspin in the pig ovarian follicles: expression and regulation by different hormones

Reproduction ◽  
2019 ◽  
Vol 158 (2) ◽  
pp. 137-148 ◽  
Author(s):  
Patrycja Kurowska ◽  
Ewa Mlyczyńska ◽  
Alix Barbe ◽  
Christophe Staub ◽  
Ewa Gregoraszczuk ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Protease Inhibitor ◽  
Serine Protease ◽  
Polycystic Ovary ◽  
Serine Protease Inhibitor ◽  
Ovarian Follicles ◽  
Oestrous Cycle ◽  
Elevated Concentration ◽  
Female Reproduction ◽  
Large White

Vaspin, also known as visceral adipose tissue-derived serine protease inhibitor, is a member of the serine protease inhibitor family. Its expression is associated with obesity, insulin resistance and type 2 diabetes, and elevated concentration is observed in polycystic ovary syndrome. However, vaspin has never been studied in the ovary. Here, we identified vaspin in two prolific breeds of pigs: fat Meishan (MS) and lean Large White (LW). We then investigated the molecular mechanism involved in the regulation of its expression in response to gonadotropins, insulin, insulin-like growth factor type 1 (IGF-1) and steroids (progesterone, testosterone and oestradiol) in ovarian follicles cells. Using real-time PCR and Western blot, we found higher vaspin mRNA and protein expression in the ovarian follicles and adipose tissue at 10–12 days of the oestrous cycle in MS compared to LW. Moreover, vaspin expression, as well as its concentration in plasma and follicular fluid, decreased in ovarian follicles of LW during days of the oestrous cycle, while the opposite results were noted in MS. Immunohistochemistry showed vaspin in granulosa, theca, cumulus cells and oocytes as well as in adipocytes. Vaspin level in the ovary increased by gonadotropin, insulin, IGF-1 and steroids stimulation through kinases JAK/Stat, ERK1/2, PI3K and AMPK, as well as factor NF-κB. These findings all show vaspin expression and regulation in the pig ovary, indicating vaspin as a new regulator in female reproduction. Future studies will be necessary for understanding the role of vaspin on ovarian physiology providing new insights into the pathology of ovaries.

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