scholarly journals Clinical and ex vivo laboratory comparison of the self-sealing properties and dimensional stability between the femtosecond laser and manual clear corneal incisions

2017 ◽  
Vol 96 (4) ◽  
pp. e510-e514 ◽  
Author(s):  
Takashi Kojima ◽  
Mari Takagi ◽  
Kei Ichikawa ◽  
Rie Horai ◽  
Yukihiro Sakai ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Dimensional Stability ◽  
Ex Vivo ◽  
The Self ◽  
Corneal Incisions

scholarly journals Femtosecond laser effect on the self-sealing properties of the corneal incision of various lengths and profile (experimental trial)

2015 ◽  
Vol 8 (2) ◽  
pp. 41-46
Author(s):  
Yulduz Shavkatovna Nizametdinova ◽  
Yury Viktorovich Takhtaev ◽  
Vadim Petrovich Nikolaenko
Keyword(s):  
Experimental Investigation ◽  
Femtosecond Laser ◽  
High Precision ◽  
The Self ◽  
Corneal Incision ◽  
Incision Length ◽  
Experimental Trial ◽  
Length Increase ◽  
Laser Effect ◽  
Corneal Incisions

An experimental investigation was carried out to study self-sealing properties of corneal incisions of different profile and length carried out with femtosecond laser Victus (Technolas Perfect Vision/Bausch&Lomb). Using femtosecond laser for this purpose allows creating corneal incisions of high precision and predictability. Reproducibility and standardization of the incision profile and length are an advantage of this technology. Obtained results showed that single-profile incisions are less stable and safe when compared to multi-profile ones. It was noted that incision length increase promotes its self-sealing properties.

scholarly journals Icariin promotes osteoblastic differentiation in OVX mice via MAPK signaling pathway revealed by profiling

2018 ◽  
Vol 01 (01) ◽  
pp. 33-41
Author(s):  
Qin Bian ◽  
Shufen Liu ◽  
Yongjian Zhao ◽  
Jianhua Huang ◽  
Ziyin Shen
Keyword(s):  
Bone Mass ◽  
Signaling Pathway ◽  
Pathway Analysis ◽  
Ex Vivo ◽  
Osteoblastic Differentiation ◽  
Mapk Signaling ◽  
Mapk Signaling Pathway ◽  
The Self ◽  
Self Renewal ◽  
Colony Forming Unit

Objective: Icariin (ICA), an extract from epimedium, has been reported to be effective in promoting bone formation. The objective of the study is to search for the molecular targets of ICA in bone mesenchymal stem cells (bMSCs) from the mice with ovariectomy (OVX)-induced osteoporosis. Methods: Six-month-old Imprinting Control Region (ICR) mice that underwent OVX were treated with ICA. After three months, bone mass was evaluated by microcomputed tomography, morphometry and immunohistological detection. bMSCs were isolated from the femur and tibia to observe the self-renewal and differentiation capacities using colony-forming unit fibroblastic (CFU-F), colony-forming unit adipocyte (CFU-Adipo) and alkaline phosphatase (ALP) staining. In addition, microarray of bMSCs ex vivo was measured two weeks after ICA treatment and analyzed by heatmap and pathway analysis. The signaling pathway was further explored by western blot assay and inhibitors of p38 and ERK: SB203508 and PD98059. Results: [Formula: see text]CT displayed a decrease in bone mass for three months after OVX. ICA treatment increased the trabecular thickness (Tb.Th), osteoblast number while decreased osteoclast number, elevating osteocalcin (OC) protein levels in vivo and facilitating the self-renewal and osteoblastic differentiation of bMSCs ex vivo. Microarray data indicated ICA rescued several gene expressions that were dysregulated by OVX. Pathway analysis revealed that the core genes acted by ICA were highly involved in MAPK signaling pathway. Further study demonstrated ICA suppressed ERK while stimulated p38 phosphorylation to promote osteoblastic differentiation in vitro. Conclusion: ICA promotes osteoblastic differentiation of bMSCs in OVX mice. MAPK signaling pathway might be involved in the process.

Maintaining the self-renewal and differentiation potential of human CD34+ hematopoietic cells using a single genetic element

Blood ◽  
2003 ◽  
Vol 102 (13) ◽  
pp. 4369-4376 ◽  
Author(s):  
James C. Mulloy ◽  
Jorg Cammenga ◽  
Francisco J. Berguido ◽  
Kaida Wu ◽  
Ping Zhou ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Ex Vivo ◽  
Severe Combined Immunodeficiency ◽  
Hematopoietic Cells ◽  
The Self ◽  
Differentiation Potential ◽  
Self Renewal ◽  
Hematopoietic Stem ◽  
Human Cd34

AbstractHematopoiesis is a complex process involving hematopoietic stem cell (HSC) self-renewal and lineage commitment decisions that must continue throughout life. Establishing a reproducible technique that allows for the long-term ex vivo expansion of human HSCs and maintains self-renewal and multipotential differentiation will allow us to better understand these processes, and we report the ability of the leukemia-associated AML1-ETO fusion protein to establish such a system. AML1-ETO-transduced human CD34+ hematopoietic cells routinely proliferate in liquid culture for more than 7 months, remain cytokine dependent for survival and proliferation, and demonstrate self-renewal of immature cells that retain both lymphoid and myeloid potential in vitro. These cells continue to express the CD34 cell surface marker and have ongoing telomerase activity with maintenance of telomere ends, however they do not cause leukemia in nonobese diabetic-severe combined immunodeficiency (NOD/SCID) mice. Identification of the signaling pathways that are modulated by AML1-ETO and lead to the self-renewal of immature human progenitor cells may assist in identifying compounds that can efficiently expand human stem and progenitor cells ex vivo. (Blood. 2003; 102:4369-4376)

scholarly journals Prediction of surgically induced astigmatism in manual and femtosecond laser-assisted clear corneal incisions

2018 ◽  
Vol 28 (4) ◽  
pp. 398-405 ◽  
Author(s):  
Joaquín Fernández ◽  
Manuel Rodríguez-Vallejo ◽  
Javier Martínez ◽  
Ana Tauste ◽  
David P Piñero
Keyword(s):  
Femtosecond Laser ◽  
Corneal Astigmatism ◽  
Toric Intraocular Lens ◽  
Surgically Induced Astigmatism ◽  
Orthogonal Components ◽  
Induced Astigmatism ◽  
The Mean ◽  
Surgically Induced ◽  
Corneal Incisions ◽  
Intraocular Lens Calculation

Purpose: To assess the surgically induced astigmatism with femtosecond laser-assisted and manual temporal clear corneal incisions and to evaluate the performance of a model for prediction of the surgically induced astigmatism based on the preoperative corneal astigmatism. Methods: Clinical data of 104 right eyes and 104 left eyes undergoing cataract surgery, 52 with manual incisions and 52 with femtosecond laser-assisted incisions in each eye group, were extracted and revised retrospectively. In all cases, manual incisions were 2.2 mm width and femtosecond incisions were 2.5 mm width, both at temporal location. A predictive model of the surgically induced astigmatism was obtained by means of simple linear regression analyses. Results: Mean surgically induced astigmatisms for right eyes were 0.14D@65° (manual) and 0.24D@92° (femtosecond) (p > 0.05) and for left eyes, 0.15D@101° (manual) and 0.19D@104° (femtosecond) (p > 0.05). The orthogonal components of the surgically induced astigmatism (XSIA, YSIA) were significantly correlated (p < 0.05) with the preoperative orthogonal components of corneal astigmatism (Xpreop, Ypreop) (r = −0.29 for X and r = −0.1 for Y). The preoperative astigmatism explained 8% of the variability of the XSIA and 3% of the variability of YSIA. The postoperative corneal astigmatism prediction was not improved by the surgically induced astigmatism obtained from the model in comparison with the simple vector subtraction of the mean surgically induced astigmatism. Conclusion: Temporal incisions induce similar astigmatism either for manual or for femtosecond procedures. This can be clinically negligible for being considered for toric intraocular lens calculation due to the great standard deviation in comparison with the mean. The usefulness of the prediction model should be confirmed in patients with high preoperative corneal astigmatism.

Arcuate Incisions in Corneal Surgery

2013 ◽  
Vol 06 (01) ◽  
pp. 26
Author(s):  
Christopher L Blanton ◽  
Keyword(s):  
Clinical Practice ◽  
Femtosecond Laser ◽  
Cataract Surgery ◽  
Current Status ◽  
Corneal Surgery ◽  
Future Developments ◽  
History Of ◽  
Correction Of Astigmatism ◽  
Indications For Use ◽  
Corneal Incisions

This article describes the current status of the femtosecond generated arcuate incision to correct corneal steepening. It covers the history of the surgical correction of astigmatism and describes the entrance of the femtosecond laser into modern corneal and cataract surgery. The process of photodisruption is explained as are the current indications for use of the femtosecond laser to make corneal incisions. A detailed narrative for programming the laser to perform arcuate incisions is included. Nomograms are referenced, and advantages over bladed incisions are described. A case is presented to show the use of these incisions in clinical practice. Finally, future developments are contemplated.

scholarly journals Burst Pressures in Eyes with Clear Corneal Incisions Treated with ReSure Glue

2021 ◽  
Vol 2021 ◽  
pp. 1-3
Author(s):  
Ahmed E. M. Shehata ◽  
Siva P. Kambhampati ◽  
Jiangxia Wang ◽  
Uri S. Soiberman
Keyword(s):  
Ex Vivo ◽  
Early Postoperative Period ◽  
Burst Pressure ◽  
Control Group ◽  
Control Groups ◽  
Nylon Suture ◽  
Significant Difference ◽  
Biodegradable Hydrogel ◽  
Suture Group ◽  
Corneal Incisions

Purpose. This study aims to measure burst pressures in 3 mm clear corneal incisions sealed with ReSure, a biodegradable hydrogel sealant, and to compare it to traditional 10-0 nylon sutures and unsealed controls. Design. An ex vivo animal study. Methods. 3 mm clear corneal incisions were performed in rabbit eyes (ex vivo). The burst pressure was determined, and then, the incisions were sealed with either ReSure glue or a single 10-0 nylon suture. Burst pressure measurements were repeated. Results. Fourteen eyes were included. The median burst pressure in the suture-control group (7 eyes) prior to suture application was 7 mmHg (range: 0–45); the median burst pressure in the 7 glue-controls was 36 mmHg (range: 5–61, p  = 0.08 for the comparison of the two control groups). The median burst pressure in the glue group was 93 mmHg (range: 39–129, p  = 0.043 when compared to glue-control). The median burst pressure in the suture group was 158 mmHg (range: 70–180, p  = 0.018 when compared to suture-control). There was no statistically significant difference in burst pressure values between the glue and suture groups ( p  = 0.08). Conclusion. In this study, ReSure glue applied to 3 mm clear corneal incisions provided sufficient resistance to elevated intraocular pressure when compared to controls. The results of this study suggest that ReSure glue may be comparable to a single 10-0 nylon suture in resisting fluid egress during the early postoperative period.

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