scholarly journals Imbalance between pro-apoptotic and pro-survival factors in human retinal pericytes in diabetic-like conditions

2017 ◽  
Vol 96 (1) ◽  
pp. e19-e26 ◽  
Author(s):  
Elena Beltramo ◽  
Ana I. Arroba ◽  
Aurora Mazzeo ◽  
Angela M. Valverde ◽  
Massimo Porta
Keyword(s):  
Survival Factors ◽  
Retinal Pericytes ◽  
Human Retinal Pericytes

scholarly journals A novel angiogenic peptide, ΔADT: A truncated adrenotensin peptide revealed by secretory peptidome analysis of human retinal pericytes

2016 ◽  
Vol 10 (6) ◽  
pp. 500-506
Author(s):  
Akinori Okumura ◽  
Eri Takahashi ◽  
Hiroyuki Unoki-Kubota ◽  
Yasushi Kaburagi
Keyword(s):  
Retinal Pericytes ◽  
Human Retinal Pericytes

scholarly journals Glucose and TGFβ2 Modulate the Viability of Cultured Human Retinal Pericytes and Their VEGF Release

2008 ◽  
Vol 33 (11-12) ◽  
pp. 984-993 ◽  
Author(s):  
Eileen K. Vidro ◽  
Stephen Gee ◽  
Richard Unda ◽  
Jian-xing Ma ◽  
Andrew Tsin
Keyword(s):  
Retinal Pericytes ◽  
Human Retinal Pericytes ◽  
Vegf Release

Thiamine and benfotiamine prevent apoptosis induced by high glucose-conditioned extracellular matrix in human retinal pericytes

2009 ◽  
Vol 25 (7) ◽  
pp. 647-656 ◽  
Author(s):  
Elena Beltramo ◽  
Konstantin Nizheradze ◽  
Elena Berrone ◽  
Sonia Tarallo ◽  
Massimo Porta
Keyword(s):  
Extracellular Matrix ◽  
High Glucose ◽  
Retinal Pericytes ◽  
Human Retinal Pericytes

scholarly journals High Glucose Induces the Loss of Retinal Pericytes Partly via NLRP3-Caspase-1-GSDMD-Mediated Pyroptosis

2020 ◽  
Vol 2020 ◽  
pp. 1-12 ◽  
Author(s):  
Jinhua Gan ◽  
Maomao Huang ◽  
Genyin Lan ◽  
Li Liu ◽  
Fangyuan Xu
Keyword(s):  
High Glucose ◽  
Pore Formation ◽  
Caspase 3 ◽  
Vision Loss ◽  
Time Dependent ◽  
Dependent Manner ◽  
Caspase 1 ◽  
Retinal Pericytes ◽  
High Glucose Medium ◽  
Human Retinal Pericytes

Diabetic retinopathy (DR) is one of the hallmark complications of diabetes and a leading cause of vision loss in adults. Retinal pericyte death seems to be a prominent feature in the onset of DR. Pyroptosis is an inflammatory form of programmed cell death, defined as being caspase-gasdermin-D (GSDMD)-dependent. The NOD-like receptor pyrin 3 (NLRP3) inflammasome plays an important role in mediating GSDMD activation. However, the role and mechanism of pyroptosis in the loss of retinal pericytes during the pathogenesis of DR are still unclear. In the present study, we cultured primary human retinal pericytes (HRPs) in high glucose medium; caspase-3 inhibitor DEVD, caspase-1 inhibitor YVAD, or NLRP3 inhibitor glyburide was used as intervention reagents; GSDMD was overexpressed or suppressed by transfection with an expressing vector or retroviral silencing of GSDMD, respectively. Our data showed that high glucose induced NLRP3-caspase-1-GSDMD activation and pore formation in a dose- and time-dependent manner (p<0.05) and resulted in the inflammatory cytokines IL-1β and IL-18 and lactate dehydrogenase (LDH) release from HRPs (p<0.05), which are all signs of HRP pyroptosis. Overexpression of GSDMD facilitated high glucose-induced pyroptosis (all p<0.05). However, these effects were blunted by synergistically treating DEVD, YVAD, and silencing GSDMD (p<0.05). Taken together, our results firstly revealed that high glucose induced the loss of retinal pericytes partly via NLRP3-caspase-1-GSDMD-mediated pyroptosis.

Somatostatin protects human retinal pericytes from inflammation mediated by microglia

2017 ◽  
Vol 164 ◽  
pp. 46-54 ◽  
Author(s):  
Aurora Mazzeo ◽  
Ana I. Arroba ◽  
Elena Beltramo ◽  
Angela M. Valverde ◽  
Massimo Porta
Keyword(s):  
Retinal Pericytes ◽  
Human Retinal Pericytes

Aflibercept, bevacizumab and ranibizumab prevent glucose-induced damage in human retinal pericytes in vitro, through a PLA2/COX-2/VEGF-A pathway

2015 ◽  
Vol 96 (3) ◽  
pp. 278-287 ◽  
Author(s):  
Giovanni Giurdanella ◽  
Carmelina Daniela Anfuso ◽  
Melania Olivieri ◽  
Gabriella Lupo ◽  
Nunzia Caporarello ◽  
...  
Keyword(s):  
Retinal Pericytes ◽  
Cox 2 ◽  
Human Retinal Pericytes
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