scholarly journals Bubble technique for Descemet membrane endothelial keratoplasty tissue preparation in an eye bank: air or liquid?

2014 ◽  
Vol 93 (2) ◽  
pp. e129-e134 ◽  
Author(s):  
Alessandro Ruzza ◽  
Mohit Parekh ◽  
Gianni Salvalaio ◽  
Stefano Ferrari ◽  
Davide Camposampiero ◽  
...  
Keyword(s):  
Tissue Preparation ◽  
Endothelial Keratoplasty ◽  
Descemet Membrane Endothelial Keratoplasty ◽  
Eye Bank ◽  
Descemet Membrane

scholarly journals Pseudophakic corneal donor tissue in Descemet membrane endothelial keratoplasty (DMEK): implications for cornea banks and surgeons

2020 ◽  
Vol 5 (1) ◽  
pp. e000524
Author(s):  
Daniel Pilger ◽  
Necip Torun ◽  
Anna-Karina B Maier ◽  
Jan Schroeter
Keyword(s):  
Endothelial Cell ◽  
Functional Results ◽  
Cell Loss ◽  
Tissue Preparation ◽  
Endothelial Keratoplasty ◽  
Descemet Membrane Endothelial Keratoplasty ◽  
Donor Tissue ◽  
Descemet Membrane ◽  
The Difference ◽  
Corneal Donor Tissue

ObjectiveIncreasingly, cornea banks are recovering donor tissue from pseudophakic donors. Little is known about their suitability for Descemet membrane endothelial keratoplasty (DMEK) surgery in terms of endothelial cell density (ECD) and preparation failure.Methods and AnalysisWe explored ECD during donor tissue preparation in 2076 grafts. Preparation failure was analysed in 1028 grafts used in DMEK surgery at our clinic. To monitor ECD and functional results, we matched 86 DMEK patients who received pseudophakic donor grafts with similar recipients of phakic donor grafts and followed them up for 36 months.ResultsAt recovery, mean ECD in pseudophakic donor grafts was 2193 cells/mm2 (SD 28.7) and 2364 cells/mm2 (SD 15.7) in phakic donor grafts (p<0.001). After cultivation, the difference increased as pseudophakic donor grafts lost 14% of ECD while phakic lost only 6% (p<0.001). At transplantation, mean ECD in pseudophakic donor grafts was 2272 cells/mm2 (SD 250) and 2370 cells/mm2 (SD 204) in phakic donor grafts (p<0.001). After transplantation, the difference in ECD increased as pseudophakic donor grafts lost 27.7% of ECD while phakic donor grafts lost only 13.3% (p<0.001). The risk of preparation failure in pseudophakic donor grafts was higher than in phakic donor grafts (OR 4.75, 95% CI 1.78 to 12.67, p=0.02). Visual acuity increased in both groups similarly.ConclusionsPseudophakic donor grafts have a lower ECD, are more prone to endothelial cell loss during recovery and surgery and are associated with a higher risk of preparation failure. Cornea banks and surgeons should consider this in the planning of graft preparation and transplantation.

Descemet Membrane Endothelial Keratoplasty Tissue Preparation From Donor Corneas Using a Standardized Submerged Hydro-separation Method

2014 ◽  
Vol 158 (2) ◽  
pp. 277-285.e1 ◽  
Author(s):  
Mohit Parekh ◽  
Alessandro Ruzza ◽  
Gianni Salvalaio ◽  
Stefano Ferrari ◽  
Davide Camposampiero ◽  
...  
Keyword(s):  
Separation Method ◽  
Tissue Preparation ◽  
Endothelial Keratoplasty ◽  
Descemet Membrane Endothelial Keratoplasty ◽  
Descemet Membrane ◽  
Donor Corneas

The Influence of Speed During Stripping in Descemet Membrane Endothelial Keratoplasty Tissue Preparation

Cornea ◽  
2020 ◽  
Vol 39 (9) ◽  
pp. 1086-1090
Author(s):  
Davide Borroni ◽  
Kunal Gadhvi ◽  
Gabriela Wojcik ◽  
Flavia Pennisi ◽  
Neeru A. Vallabh ◽  
...  
Keyword(s):  
Tissue Preparation ◽  
Endothelial Keratoplasty ◽  
Descemet Membrane Endothelial Keratoplasty ◽  
Descemet Membrane

Donor Tissue Preparation for Descemet Membrane Endothelial Keratoplasty

Cornea ◽  
2018 ◽  
Vol 37 (1) ◽  
pp. 128-135 ◽  
Author(s):  
Rénuka S. Birbal ◽  
Shameema Sikder ◽  
Jessica T. Lie ◽  
Esther A. Groeneveld-van Beek ◽  
Silke Oellerich ◽  
...  
Keyword(s):  
Tissue Preparation ◽  
Endothelial Keratoplasty ◽  
Descemet Membrane Endothelial Keratoplasty ◽  
Donor Tissue ◽  
Descemet Membrane

Comment on donor tissue preparation for Descemet membrane endothelial keratoplasty

2009 ◽  
Vol 35 (3) ◽  
pp. 407-408 ◽  
Author(s):  
Ioannis Athanasiadis ◽  
Deric DeWit ◽  
Anant Sharma ◽  
Chitra Sambare ◽  
Baddar M.H. Al Barwani
Keyword(s):  
Tissue Preparation ◽  
Endothelial Keratoplasty ◽  
Descemet Membrane Endothelial Keratoplasty ◽  
Donor Tissue ◽  
Descemet Membrane

scholarly journals Preclinical testing of small diameter Descemet membrane endothelial keratoplasty grafts to increase tissue availability

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0246516
Author(s):  
Sorcha Ní Dhubhghaill ◽  
Alina Miron ◽  
Jessica T. Lie ◽  
Isabel Dapena ◽  
Silke Oellerich ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Small Diameter ◽  
Cell Loss ◽  
Endothelial Keratoplasty ◽  
Descemet Membrane Endothelial Keratoplasty ◽  
Eye Bank ◽  
Donor Pool ◽  
Descemet Membrane

In this study, we describe a process of preparing, surgically manipulating, and validating a novel “small diameter” 4mm circular Descemet membrane endothelial keratoplasty (DMEK) graft in vitro. Three small diameter DMEK grafts can be prepared from a single donor endothelium and could, therefore, potentially expand the donor pool. Prior to clinical use, however, we aimed to examine each step of the process to determine the effect on the endothelial cell loss and whether or not cells retained their capacity to migrate uniformly. For this study, circular small diameter grafts, obtained from twelve corneas of ten donors deemed ineligible for transplantation, were included. Small diameter DMEK graft preparation was successful in all cases (n = 36). Endothelial cell density (ECD), determined in the eye bank on seventeen grafts, showed an average decrease from 2413 (±189) cells/mm2 before to 2240 (±413) cells/mm2 after preparation. Twenty-four grafts were used to simulate DMEK-surgery in vitro and were successfully stained with 0.06% trypan blue, loaded into a straight DMEK-injector, unfolded, positioned, and centered within the circular ~ 4mm descemetorhexis. The estimated % area populated by viable cells on the grafts decreased from on average 92 (±3) % before to 78 (±10) % (n = 4) after in vitro surgery. Cells displayed a capacity for uniform cell migration from all edges of the graft (n = 4) when embedded in the 3D hydrogel system. Our data show, that by using an in vitro model of DMEK-surgery it was possible to test the 4mm circular DMEK grafts from eye bank preparation to surgical implantation. The cell loss after in vitro surgery was comparable with the in vivo ECD decline early after DMEK and the capacity of the cells to migrate to potentially cover bare stroma indicates that these small diameter grafts may be a viable clinical option to treat central endothelial disease.

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