scholarly journals Down‐regulated tear film proteins in neovascular age‐related macular degeneration are involved in the regulation of protein clearance, inflammation, neovascularization and apoptosis

2021 ◽  
Vol 99 (S265) ◽  
Author(s):  
Mateusz Winiarczyk ◽  
Dagmara Winiarczyk ◽  
Katarzyna Michalak ◽  
Kai Kaarniranta ◽  
Jerzy Mackiewicz ◽  
...  
Keyword(s):  
Macular Degeneration ◽  
Tear Film ◽  
Age Related Macular Degeneration ◽  
Protein Clearance ◽  
Age Related

scholarly journals Dysregulated Tear Film Proteins in Macular Edema Due to the Neovascular Age-Related Macular Degeneration Are Involved in the Regulation of Protein Clearance, Inflammation, and Neovascularization

2021 ◽  
Vol 10 (14) ◽  
pp. 3060
Author(s):  
Mateusz Winiarczyk ◽  
Dagmara Winiarczyk ◽  
Katarzyna Michalak ◽  
Kai Kaarniranta ◽  
Łukasz Adaszek ◽  
...  
Keyword(s):  
Macular Degeneration ◽  
Macular Edema ◽  
Tear Film ◽  
Developed Countries ◽  
Age Related Macular Degeneration ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Neovascular Amd ◽  
Protein Clearance ◽  
Age Related

Macular edema and its further complications due to the leakage from the choroidal neovascularization in course of the age-related macular degeneration (AMD) is a leading cause of blindness among elderly individuals in developed countries. Changes in tear film proteomic composition have been reported to occur in various ophthalmic and systemic diseases. There is an evidence that the acute form of neovascular AMD may be reflected in the tear film composition. Tear film was collected with Schirmer strips from patients with neovascular AMD and sex- and age-matched control patients. Two-dimensional electrophoresis was performed followed by MALDI-TOF mass spectrometry for identification of differentially expressed proteins. Quantitative analysis of the differential electrophoretic spots was performed with Delta2D software. Altogether, 11 significantly differentially expressed proteins were identified; of those, 8 were downregulated, and 3 were upregulated in the tear film of neovascular AMD patients. The differentially expressed proteins identified in tear film were involved in signaling pathways associated with impaired protein clearance, persistent inflammation, and neovascularization. Tear film protein analysis is a novel way to screen AMD-related biomarkers.

scholarly journals Differential Expression of Inflammasome-Related Genes in Induced Pluripotent Stem-Cell-Derived Retinal Pigment Epithelial Cells with or without History of Age-Related Macular Degeneration

2021 ◽  
Vol 22 (13) ◽  
pp. 6800
Author(s):  
Maria Hytti ◽  
Eveliina Korhonen ◽  
Heidi Hongisto ◽  
Kai Kaarniranta ◽  
Heli Skottman ◽  
...  
Keyword(s):  
Macular Degeneration ◽  
Retinal Pigment ◽  
Induced Pluripotent Stem Cell ◽  
Retinal Pigment Epithelial Cells ◽  
Age Related Macular Degeneration ◽  
Inflammasome Activation ◽  
Protein Clearance ◽  
Age Related ◽  
Induced Pluripotent ◽  
Pigment Epithelial Cells

Inflammation is a key underlying factor of age-related macular degeneration (AMD) and inflammasome activation has been linked to disease development. Induced pluripotent stem-cell-derived retinal pigment epithelial cells (iPSC-RPE) are an attractive novel model system that can help to further elucidate disease pathways of this complex disease. Here, we analyzed the effect of dysfunctional protein clearance on inflammation and inflammasome activation in iPSC-RPE cells generated from a patient suffering from age-related macular degeneration (AMD) and an age-matched control. We primed iPSC-RPE cells with IL-1α and then inhibited both proteasomal degradation and autophagic clearance using MG-132 and bafilomycin A1, respectively, causing inflammasome activation. Subsequently, we determined cell viability, analyzed the expression levels of inflammasome-related genes using a PCR array, and measured the levels of pro-inflammatory cytokines IL-1β, IL-6, IL-8, and MCP-1 secreted into the medium. Cell treatments modified the expression of 48 inflammasome-related genes and increased the secretion of mature IL-1β, while reducing the levels of IL-6 and MCP-1. Interestingly, iPSC-RPE from an AMD donor secreted more IL-1β and expressed more Hsp90 prior to the inhibition of protein clearance, while MCP-1 and IL-6 were reduced at both protein and mRNA levels. Overall, our results suggest that cellular clearance mechanisms might already be dysfunctional, and the inflammasome activated, in cells with a disease origin.

scholarly journals Ocular Surface Temperature in Age-Related Macular Degeneration

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Andrea Sodi ◽  
Sara Matteoli ◽  
Giovanni Giacomelli ◽  
Lucia Finocchio ◽  
Andrea Corvi ◽  
...  
Keyword(s):  
Surface Temperature ◽  
Macular Degeneration ◽  
Ocular Surface ◽  
Tear Film ◽  
Ocular Blood Flow ◽  
Processing Technique ◽  
Age Related Macular Degeneration ◽  
Image Processing Technique ◽  
Control Group ◽  
Age Related

Background. The aim of this study is to investigate the ocular thermographic profiles in age-related macular degeneration (AMD) eyes and age-matched controls to detect possible hemodynamic abnormalities, which could be involved in the pathogenesis of the disease.Methods. 32 eyes with early AMD, 37 eyes with atrophic AMD, 30 eyes affected by untreated neovascular AMD, and 43 eyes with fibrotic AMD were included. The control group consisted of 44 healthy eyes. Exclusion criteria were represented by any other ocular diseases other than AMD, tear film abnormalities, systemic cardiovascular abnormalities, diabetes mellitus, and a body temperature higher than 37.5°C. A total of 186 eyes without pupil dilation were investigated by infrared thermography (FLIR A320). The ocular surface temperature (OST) of three ocular points was calculated by means of an image processing technique from the infrared images. Two-samplet-test and one-way analysis of variance (ANOVA) test were used for statistical analyses.Results. ANOVA analyses showed no significant differences among AMD groups (Pvalue >0.272). OST in AMD patients was significantly lower than in controls (P>0.05).Conclusions. Considering the possible relationship between ocular blood flow and OST, these findings might support the central role of ischemia in the pathogenesis of AMD.

scholarly journals Tear film proteome in age-related macular degeneration

2018 ◽  
Vol 256 (6) ◽  
pp. 1127-1139 ◽  
Author(s):  
Mateusz Winiarczyk ◽  
Kai Kaarniranta ◽  
Stanisław Winiarczyk ◽  
Łukasz Adaszek ◽  
Dagmara Winiarczyk ◽  
...  
Keyword(s):  
Macular Degeneration ◽  
Tear Film ◽  
Age Related Macular Degeneration ◽  
Age Related

Age related macular degeneration (ARMD) – pathophysiological and clinical features and therapeutic concepts

2001 ◽  
Vol 58 (1) ◽  
pp. 28-35 ◽  
Author(s):  
Ursula Körner-Stiefbold
Keyword(s):  
Macular Degeneration ◽  
Clinical Features ◽  
Altersbedingte Makuladegeneration ◽  
Age Related Macular Degeneration ◽  
Genetische Faktoren ◽  
Age Related ◽  
Therapeutic Concepts

Die altersbedingte Makuladegeneration (AMD) ist eine der häufigsten Ursachen für einen irreversiblen Visusverlust bei Patienten über 65 Jahre. Nahezu 30% der über 75-Jährigen sind von einer AMD betroffen. Trotz neuer Erkenntnisse in der Grundlagenforschung ist die Ätiologie, zu der auch genetische Faktoren gehören, noch nicht völlig geklärt. Aus diesem Grund sind die Behandlungsmöglichkeiten zum jetzigen Zeitpunkt noch limitiert, so dass man lediglich von Therapieansätzen sprechen kann. Die derzeit zur Verfügung stehenden Möglichkeiten wie medikamentöse, chirurgische und laser- und strahlentherapeutische Maßnahmen werden beschrieben.

Nutrition in Age-Related Macular Degeneration Survey

2018 ◽  
Author(s):  
Rebekah Stevens ◽  
Richard Cooke ◽  
Hannah Bartlett
Keyword(s):  
Macular Degeneration ◽  
Age Related Macular Degeneration ◽  
Age Related
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