Extended ex vivo normothermic perfusion for preservation of vascularized composite allografts

2020 ◽  
Vol 44 (8) ◽  
pp. 846-855
Author(s):  
Vahe Fahradyan ◽  
Sayf Al‐deen Said ◽  
Carlos Ordenana ◽  
Edoardo Dalla Pozza ◽  
Russell Frautschi ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Normothermic Perfusion

scholarly journals Temperature Controlled and Monitored Ex Vivo Lung Perfusion System for Research and Training Purposes

2019 ◽  
Vol 5 (1) ◽  
pp. 293-295
Author(s):  
Christina Pongratz ◽  
Jens Ziegle ◽  
Axel Boese ◽  
Michael Friebe ◽  
Helena Linge ◽  
...  
Keyword(s):  
Storage Temperature ◽  
Ex Vivo ◽  
Culture Conditions ◽  
Lung Perfusion ◽  
Tissue Storage ◽  
Ex Vivo Lung Perfusion ◽  
Device Development ◽  
Preservation Method ◽  
Normothermic Perfusion ◽  
Short Period

AbstractEx vivo lung perfusion (EVLP) is a preservation method for donor lungs, which keep lungs viable in a physiological environment outside of a body for a short period of time. EVLP is established clinically for lung transplantation. Experimental applications for EVLP are e.g. lung cancer research or medical device development and testing. For preservation, a lung is ventilated artificially in an organ chamber and perfused antegrade through the pulmonary artery. Here we introduce a thermoregulation system for an experimental EVLP system to be used for translational research approaches as well as for training medical staff. To implement physiological culture conditions that are a prerequisite for lung preservation and tissue homeostasis, a thermoregulation is needed to rewarm the explanted lung tissue (storage temperature 4°C). Technically, the EVLP system must be thermally insulated, so loss of caloric is avoided. For monitoring, temperature sensors are integrated within the lung, in the organ chamber and in the afferent perfusate tube, whereby the measured values determine the thermoregulation. Initial tests using thermal packs (cooled to 4-6°C) placed on a heating mat, as a part of the perfusion circuit, showed that the perfusate temperature falls to 34°C, but restores after approximately 60 minutes (36.5°C), whereby the thermal pack is warmed. With this setup longer perfusion times should be obtained rather than without thermoregulation due to normothermic perfusion of the lung.

scholarly journals 26-hour Storage of a Declined Liver Before Successful Transplantation Using Ex Vivo Normothermic Perfusion

2017 ◽  
Vol 265 (1) ◽  
pp. e1-e2 ◽  
Author(s):  
Christopher J. E. Watson ◽  
Lucy V. Randle ◽  
Vasilis Kosmoliaptsis ◽  
Paul Gibbs ◽  
Michael Allison ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Normothermic Perfusion ◽  
Successful Transplantation

Twenty-Four-Hour Normothermic Perfusion of Isolated Ex Vivo Hearts Using Plasma Exchange

2020 ◽  
Author(s):  
Lise Tchouta ◽  
Daniel Drake ◽  
Mark Hoenerhoff ◽  
Alvaro Rojas-Pena ◽  
Jonathan Haft ◽  
...  
Keyword(s):  
Plasma Exchange ◽  
Ex Vivo ◽  
Normothermic Perfusion

Ex-vivo Hyperbaric Normothermic Perfusion for 24 Hours Mitigates Reperfusion Injury in Porcine Vascular Composite Allotransplantation (VCA)

2018 ◽  
Vol 102 ◽  
pp. S713
Author(s):  
Nicholas Robbins ◽  
Matthew J Wordsworth ◽  
Michael R Sippel ◽  
Bijaya K Parida ◽  
Vijay S Gorantla ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Ex Vivo ◽  
Normothermic Perfusion

Ex-Vivo normothermic perfusion of “Antifreeze” kidneys

Cryobiology ◽  
2018 ◽  
Vol 81 ◽  
pp. 232
Author(s):  
Junichiro Sageshima ◽  
Ivonne Palma ◽  
Andrew Perry ◽  
Richard Perez ◽  
Kuang-Yu Jen
Keyword(s):  
Ex Vivo ◽  
Normothermic Perfusion

Ex-vivo normothermic perfusion in renal transplantation

2016 ◽  
Vol 21 (3) ◽  
pp. 301-307 ◽  
Author(s):  
Cissy Yong ◽  
Sarah A. Hosgood ◽  
Michael L. Nicholson
Keyword(s):  
Renal Transplantation ◽  
Ex Vivo ◽  
Normothermic Perfusion
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