Hydrogen Gas Ameliorates Hepatic Reperfusion Injury After Prolonged Cold Preservation in Isolated Perfused Rat Liver

2016 ◽  
Vol 40 (12) ◽  
pp. 1128-1136 ◽  
Author(s):  
Shingo Shimada ◽  
Kenji Wakayama ◽  
Moto Fukai ◽  
Tsuyoshi Shimamura ◽  
Takahisa Ishikawa ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Rat Liver ◽  
Hydrogen Gas ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Cold Preservation

scholarly journals Heavy Water (D2O) Containing Preservation Solution Reduces Hepatic Cold Preservation and Reperfusion Injury in an Isolated Perfused Rat Liver (IPRL) Model

2019 ◽  
Vol 8 (11) ◽  
pp. 1818
Author(s):  
Shingo Shimada ◽  
Moto Fukai ◽  
Kengo Shibata ◽  
Sodai Sakamoto ◽  
Kenji Wakayama ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reperfusion Injury ◽  
Rat Liver ◽  
Heavy Water ◽  
Biological Effects ◽  
Energy Charge ◽  
Liver Graft ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Cold Preservation

Background: Heavy water (D2O) has many biological effects due to the isotope effect of deuterium. We previously reported the efficacy of D2O containing solution (Dsol) in the cold preservation of rat hearts. Here, we evaluated whether Dsol reduced hepatic cold preservation and reperfusion injury. Methods: Rat livers were subjected to 48-hour cold storage in University of Wisconsin (UW) solution or Dsol, and subsequently reperfused on an isolated perfused rat liver. Graft function, injury, perfusion kinetics, oxidative stress, and cytoskeletal integrity were assessed. Results: In the UW group, severe ischemia and reperfusion injury (IRI) was shown by histopathology, higher liver enzymes leakage, portal resistance, and apoptotic index, oxygen consumption, less bile production, energy charge, and reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio (versus control). The Dsol group showed that these injuries were significantly ameliorated (versus the UW group). Furthermore, cytoskeletal derangement was progressed in the UW group, as shown by less degradation of α-Fodrin and by the inactivation of the actin depolymerization pathway, whereas these changes were significantly suppressed in the Dsol group. Conclusion: Dsol reduced hepatic IRI after extended cold preservation and subsequent reperfusion. The protection was primarily due to the maintenance of mitochondrial function, cytoskeletal integrity, leading to limiting oxidative stress, apoptosis, and necrosis pathways.

Protective effects of regulatory amino acids on ischaemia-reperfusion injury in the isolated perfused rat liver

2006 ◽  
Vol 41 (11) ◽  
pp. 1342-1349 ◽  
Author(s):  
Heidi Schuster ◽  
Marie-Céline Blanc ◽  
Nathalie Neveux ◽  
Dominique Bonnefont-Rousselot ◽  
Agnès Le Tourneau ◽  
...  
Keyword(s):  
Amino Acids ◽  
Reperfusion Injury ◽  
Rat Liver ◽  
Isolated Perfused Rat Liver ◽  
Protective Effects ◽  
Perfused Rat Liver ◽  
Ischaemia Reperfusion Injury ◽  
Ischaemia Reperfusion

Verapamil and flunarizine protect the isolated perfused rat liver against warm ischemia and reperfusion injury

1995 ◽  
Vol 195 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Thomas Konrad ◽  
Christian Bloechle ◽  
Garry Haller ◽  
Christoph E. Broelsch ◽  
Klaus Henning Usadel ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Rat Liver ◽  
Warm Ischemia ◽  
Isolated Perfused Rat Liver ◽  
Ischemia And Reperfusion ◽  
Perfused Rat Liver ◽  
Ischemia And Reperfusion Injury

A Comparison of Plasminogen Activators Derived from Rat Plasma, Primary Rat Hepatocytes and Isolated Perfused Rat Liver

1982 ◽  
Vol 47 (02) ◽  
pp. 166-172 ◽  
Author(s):  
Yoav Sharoni ◽  
Maria C Topal ◽  
Patricia R Tuttle ◽  
Henry Berger
Keyword(s):  
Rat Liver ◽  
Isoelectric Point ◽  
Rat Hepatocytes ◽  
Cell Types ◽  
Plasminogen Activators ◽  
Rat Plasma ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Molecular Weights ◽  
Physiological Relevance

SummaryOf the two cell types it was possible to culture from the dissociated rat liver, hepatocytes and Kupffer cells, only the former were fibrinolytically active. Rat hepatocytes during the first 24 hr in culture secreted two plasminogen activators with molecular weights identical to those found in rat plasma, an 80,000-dalton form (PA-80) and a 45,000-dalton form (PA-45). Partially purified preparations of plasminogen activators from both sources were subjected to isoelectric focusing (IEF) to compare characteristics further. There were three distinct peaks of PA-45 in each preparation with isoelectric points of 7.1, 7.2 and 7.4; all electrophoretic forms had the same low affinity to fibrin. PA-80 from both sources displayed similar IEF profiles with forms ranging from pH values of 7 to 8, all with the same high affinity to fibrin. The major form of PA-80 in the plasma preparation had an isoelectric point of 7.9 whereas that in the hepatocyte preparation had an isoelectric point of 7.6. The isolated perfused rat liver was also shown to produce both PA-80 and PA-45 emphasizing the physiological relevance of the findings with hepatocytes. It is concluded that in the rat hepatocytes contribute to the plasma profile with regard to the plasminogen activator content.

Sign in / Sign up

Export Citation Format

Share Document