High dietary lipid level decreases the immunity and disease resistance of abalone Haliotis discus hannai and affects the perilipin‐2/TLR4, JNK and Keap1/Nrf2 pathways

2021 ◽  
Author(s):  
Yanlin Guo ◽  
Dong Huang ◽  
Mingzhu Pan ◽  
Mengxi Yang ◽  
Kai Luo ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Lipid Level ◽  
Dietary Lipid ◽  
Haliotis Discus Hannai ◽  
Dietary Lipid Level ◽  
Haliotis Discus ◽  
Perilipin 2 ◽  
Abalone Haliotis Discus

scholarly journals Functions of Forkhead Box O on Glucose Metabolism in Abalone Haliotis discus hannai and Its Responses to High Levels of Dietary Lipid

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 297
Author(s):  
Liu Wang ◽  
Yanlin Guo ◽  
Mingzhu Pan ◽  
Xinxin Li ◽  
Dong Huang ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Mrna Level ◽  
Glycogen Metabolism ◽  
Dietary Lipid ◽  
Haliotis Discus Hannai ◽  
Forkhead Transcription Factor ◽  
Forkhead Box ◽  
Haliotis Discus ◽  
Abalone Haliotis Discus

The forkhead box O (FoxO) subfamily is a member of the forkhead transcription factor family. It has regulation functions in glucose metabolism in mammals and fish. In the present study, a gene of the foxo homolog in abalone Haliotis discus hannai was cloned. A conservative forkhead (FH) domain and a transactivation (FoxO-TAD) domain were identified. Abalone foxo-specific siRNA (small interfering RNA) was injected to investigate the functions of foxo on glucose metabolism. Knockdown of foxo inhibited expression of phosphoenolpyruvate carboxykinase (pepck) and significantly increased expressions of hexokinase (hk) and pyruvate kinase (pk), but it failed to inhibit the relative mRNA level of glucose-6-phosphatase (g6pase). Then, a 100-day feeding trial was conducted to investigate the response of foxo and glucose metabolism in abalone fed with 1.57% (LFD, low-fat diet), 3.82% (MFD, middle-fat diet) and 6.72% (HFD, high-fat diet) of dietary lipid, respectively. The insulin-signaling pathway (AKT) was depressed and FoxO was activated by the HFD, but it did not inhibit glycolysis (hk) or improved gluconeogenesis significantly (pepck and g6pase). At the same time, impaired hepatopancreas glycogen storage raised hemolymph glucose levels. In conclusion, abalone foxo can be regulated by dietary lipid and can regulate gluconeogenesis or glycolysis in response to changes of dietary lipid levels, in which glycogen metabolism plays an important role.

Identification of growth-related SNPs and genes in the genome of the Pacific abalone (Haliotis discus hannai) using GWAS

Aquaculture ◽  
2021 ◽  
Vol 541 ◽  
pp. 736820
Author(s):  
Wenzhu Peng ◽  
Feng Yu ◽  
Yiyu Wu ◽  
Yifang Zhang ◽  
Chengkuan Lu ◽  
...  
Keyword(s):  
Haliotis Discus Hannai ◽  
Pacific Abalone ◽  
The Pacific ◽  
Haliotis Discus ◽  
Abalone Haliotis Discus
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