Antioxidant-related parameters, digestive enzyme activity and intestinal morphology in rainbow trout (Oncorhynchus mykiss ) fry fed graded levels of red seaweed, Gracilaria pygmaea

2017 ◽  
Vol 24 (2) ◽  
pp. 777-785 ◽  
Author(s):  
E. Sotoudeh ◽  
F. Mardani
Keyword(s):  
Enzyme Activity ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Digestive Enzyme ◽  
Intestinal Morphology ◽  
Digestive Enzyme Activity ◽  
Red Seaweed ◽  
Rainbow Trout Oncorhynchus Mykiss

Effects of different photoperiods on digestive enzyme activities in rainbow trout (Oncorhynchus mykiss) alevin and fry

2016 ◽  
Vol 94 (6) ◽  
pp. 435-442 ◽  
Author(s):  
Fereshteh Ramzanzadeh ◽  
Sakineh Yeganeh ◽  
Khosro JaniKhalili ◽  
Seyedeh Sedigheh Babaei
Keyword(s):  
Enzyme Activity ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Enzyme Activities ◽  
Digestive Enzyme ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
First Feeding ◽  
Significant Difference ◽  
Digestive Enzyme Activities ◽  
L 14

The aim of this study was to investigate the ontogeny of digestive enzymes and effects of photoperiod on pancreatic enzyme activity in rainbow trout (Oncorhynchus mykiss (Walbaum, 1792)) alevin and fry. After hatching, 3600 individuals of first-feeding alevin with a mean (±SD) mass of 119 ± 0.01 mg were reared in plastic tanks at four photoperiod levels (llight (L) : dark (D) cycle) for 6 weeks. Each replicate contained 300 alevin. Photoperiod treatments consisted of 14 h L : 10 h D, 10 h L : 14 h D, 4 h L : 20 h D, and 24 h L : 0 h D. During the rearing period, environmental variables including water temperature, oxygen, and pH were checked. Random sampling of alevin and fry was done at 0, 1, 3, 5, 8, 10, 15, 20, 25, 30, 39, and 44 days after the beginning of the first feeding (equal to 18, 19, 23, 25, 28, 30, 35, 40, 45, 50, 58, 63 days post hatching (dph)). The digestive enzyme activities in alevin and fry had a similar variation trend with age at all photoperiod levels. The specific activities of trypsin, chymotrypsin, and α-amylase at 24 h L : 0 h D were significantly higher than other treatments, but there was no significant difference in specific activity of lipase among treatments. The results demonstrated that growth, feeding efficiency, and digestive enzyme activity improved with longer exposure to photoperiod in rearing conditions.

Inter-tissue differences in mitochondrial enzyme activity, RNA and DNA in rainbow trout (Oncorhynchus mykiss)

1998 ◽  
Vol 201 (24) ◽  
pp. 3377-3384 ◽  
Author(s):  
S. C. Leary ◽  
B. J. Battersby ◽  
C. D. Moyes
Keyword(s):  
Enzyme Activity ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Citrate Synthase ◽  
Mitochondrial Protein ◽  
Molecular Organization ◽  
Mitochondrial Rna ◽  
Mitochondrial Enzyme ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
The Relationship

We examined whether the relationships between mitochondrial enzyme activity, mitochondrial DNA (mtDNA) and mitochondrial RNA (mtRNA) were conserved in rainbow trout (Oncorhynchus mykiss) tissues that differ widely in their metabolic and molecular organization. The activity of citrate synthase (CS), expressed either per gram of tissue or per milligram of total DNA, indicated that these tissues (blood, brain, kidney, liver,cardiac, red and white muscles) varied more than 100-fold in mitochondrial content. Several-fold differences in the levels of CS mRNA per milligram of DNA and CS activity per CS mRNA were also observed, suggesting that fundamental differences exist in the regulation of CS levels across tissues. Although tissues varied 14-fold in RNA g-1, poly(A+) RNA (mRNA)was approximately 2 % of total RNA in all tissues. DNA g-1 also varied 14-fold across tissues, but RNA:DNA ratios varied only 2.5-fold. The relationship between two mitochondrial mRNA species (COX I, ATPase VI) and one mitochondrial rRNA (16S) species was constant across tissues. The ratio of mtRNA to mtDNA was also preserved across most tissues; red and white muscle had 10- to 20-fold lower levels of mtDNA g-1 but 7- to 10-fold higher mtRNA:mtDNA ratios, respectively. Collectively, these data suggest that the relationship between mitochondrial parameters is highly conserved across most tissues, but that skeletal muscles differ in a number of important aspects of respiratory gene expression ('respiratory genes'include genes located on mtDNA and genes located in the nucleus that encode mitochondrial protein) and mtDNA transcriptional regulation.

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