scholarly journals An evaluation of increasing linolenic acid level in the diet of Macrobrachium nipponense : Lipid deposition, fatty acid composition and expression of lipid metabolism-related genes

2017 ◽  
Vol 24 (2) ◽  
pp. 758-767 ◽  
Author(s):  
N. Luo ◽  
Z.-L. Ding ◽  
Y.-Q. Kong ◽  
R.-F. Zhang ◽  
Y.-X. Zhang ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Lipid Metabolism ◽  
Linolenic Acid ◽  
Acid Composition ◽  
Acid Level ◽  
Lipid Deposition ◽  
Macrobrachium Nipponense ◽  
Linolenic Acid Level

The effect of lipid metabolism-related genes on intramuscular fat content and fatty acid composition in multiple muscles

2018 ◽  
Vol 58 (11) ◽  
pp. 2003 ◽  
Author(s):  
Chendong Liu ◽  
Linyuan Shen ◽  
Jingjing Du ◽  
Xiaoqian Wu ◽  
Jia Luo ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Lipid Metabolism ◽  
Muscle Fibre ◽  
Meat Quality ◽  
Acid Composition ◽  
Fat Content ◽  
Muscle Fibre Types ◽  
Lipid Deposition ◽  
Intramuscular Fat Content

Intramuscular fat content (IMF) and fatty acid composition are two important factors that have a significant effect on meat quality. Previous studies about lipid deposition mainly focussed on breed effects, but the regulation mechanism of lipid metabolism among multiple muscles is not clear. Here, we hypothesised that there are correlations between lipid metabolism-related genes and muscle fibre types composition and lipid deposition in multiple muscles. We analysed the relationship between the expression of 18 lipid metabolism-related genes and muscle fibre types composition, and their relation with IMF and fatty acid composition in 14 different muscles. The IMF content and fatty acid composition were significantly different among the muscle tissues (P < 0.01). IMF was significantly higher in the trapezius and semitendinosus muscles compared with the others (P < 0.05); the content was about four times higher than that of the peroneal longus. Moreover, the trapezius and masseter had a higher monounsaturated fatty acid (MUFA) : saturated fatty acid (SFA) (>1.48) (P < 0.05) and polyunsaturated fatty acid (PUFA) : SFA (>0.45) (P < 0.05), which are more conducive to human health. Second, the expression levels of seven genes, AdPLA (r = 0.605, P < 0.05), DGAT2 (r = 0.553, P < 0.05), FABP4 (r = 0.637, P < 0.05), ELOVL6 (r = 0.57, P < 0.05), FASN (r = 0.556, P < 0.05), PPARγ (r = –0.51, P < 0.05) and SCD (r = 0.579, P < 0.05) were associated with IMF. In addition, the expression of FASN was positively correlated with MUFA (r = 0.556, P < 0.05) and total fatty acids (r = 0.547, P < 0.05). Finally, the MyHC IIa content (IIa) was positively correlated with MUFA (r = 0.56, P < 0.05) but negatively correlated with SFA (r = –0.553, P < 0.05) in different muscles. Besides, MUFA : SFA was positively correlated with IIa (r = 0.609, P < 0.05), although negatively correlated with MyHC IIx content (IIx) (r = –0.566, P < 0.05). These results added beneficial information to improve meat quality, and our understanding of the mechanism of fat deposition and fatty acid composition regulation. They also provide potential genetic markers for the study of muscular fatty acid composition.

scholarly journals A grape polyphenol extract modulates muscle membrane fatty acid composition and lipid metabolism in high-fat–high-sucrose diet-fed rats

2011 ◽  
Vol 106 (4) ◽  
pp. 491-501 ◽  
Author(s):  
Manar Aoun ◽  
Francoise Michel ◽  
Gilles Fouret ◽  
Audrey Schlernitzauer ◽  
Vincent Ollendorff ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Lipid Metabolism ◽  
Protein Expression ◽  
Acid Composition ◽  
Phospholipid Fatty Acid ◽  
Phospholipid Fatty Acid Composition ◽  
High Fat ◽  
Gene And Protein Expression ◽  
High Sucrose

Accumulation of muscle TAG content and modification of muscle phospholipid fatty acid pattern may have an impact on lipid metabolism, increasing the risk of developing diabetes. Some polyphenols have been reported to modulate lipid metabolism, in particular those issued from red grapes. The present study was designed to determine whether a grape polyphenol extract (PPE) modulates skeletal muscle TAG content and phospholipid fatty acid composition in high-fat–high-sucrose (HFHS) diet-fed rats. Muscle plasmalemmal and mitochondrial fatty acid transporters, GLUT4 and lipid metabolism pathways were also explored. The PPE decreased muscle TAG content in HFHS/PPE diet-fed rats compared with HFHS diet-fed rats and induced higher proportions of n-3 PUFA in phospholipids. The PPE significantly up-regulated GLUT4 mRNA expression. Gene and protein expression of muscle fatty acid transporter cluster of differentiation 36 (CD36) was increased in HFHS diet-fed rats but returned to control values in HFHS/PPE diet-fed rats. Carnitine palmitoyltransferase 1 protein expression was decreased with the PPE. Mitochondrial β-hydroxyacyl CoA dehydrogenase was increased in HFHS diet-fed rats and returned to control values with PPE supplementation. Lipogenesis, mitochondrial biogenesis and mitochondrial activity were not affected by the PPE. In conclusion, the PPE modulated membrane phospholipid fatty acid composition and decreased muscle TAG content in HFHS diet-fed rats. The PPE lowered CD36 gene and protein expression, probably decreasing fatty acid transport and lipid accumulation within skeletal muscle, and increased muscle GLUT4 expression. These effects of the PPE are in favour of a better insulin sensibility.

Influence of herbage species, cultivar and cutting date on fatty acid composition of herbage and lipid metabolism during ensiling

2009 ◽  
Vol 64 (2) ◽  
pp. 196-207 ◽  
Author(s):  
G. Van Ranst ◽  
V. Fievez ◽  
M. Vandewalle ◽  
J. De Riek ◽  
E. Van Bockstaele
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Lipid Metabolism ◽  
Acid Composition

scholarly journals Adipose Fatty Acid Composition and Rate of Incorporation of α-Linolenic Acid Differ between Normal and Lipoprotein Lipase-Deficient Cats

2006 ◽  
Vol 136 (12) ◽  
pp. 2980-2986 ◽  
Author(s):  
Brian C. Veltri ◽  
Robert C. Backus ◽  
Quinton R. Rogers ◽  
Edward J. DePeters
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linolenic Acid ◽  
Lipoprotein Lipase ◽  
Acid Composition

scholarly journals Effects of dietary n -6: n -3 PUFA ratio on the growth performance, serum lipid and adipokine profiles, tissue fatty acid composition and gene expression of lipid metabolism of finishing pigs

2020 ◽  
Author(s):  
Jing Chen ◽  
Jiantao Li ◽  
Xianjun Liu ◽  
Hongze Cui ◽  
Liyan Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Lipid Metabolism ◽  
Growth Performance ◽  
Acid Composition ◽  
Blood Lipid ◽  
Finishing Pigs ◽  
Pufa Ratio ◽  
Tissue Fatty Acid Composition

Abstract Background The health benefits of n-3 polyunsaturated fatty acids (PUFA), such as cardioprotective, anti-inflammatory and hypo-triglyceridemic properties, have been well documented. However, current dietary modification can easily lead to excessive concentrations of n-6 PUFA and an imbalance in the n-6:n-3 PUFA ratio, resulting in the pathogenesis of obesity and related diseases. This study investigated the effects of various dietary n-6:n-3 PUFA ratios on the growth performance, blood lipid and cytokine profiles, tissue fatty acid composition, and gene expression involved in lipid metabolism in finishing pigs. Seventy-two crossbred [(Duroc × Landrace) × Yorkshire] finishing pigs (68.5 ± 1·8 kg) were fed one of four isoenergetic diets with n-6:n-3 PUFA ratios of 2:1, 3:1, 5:1 and 8:1. Results Pigs fed diets with n-6:n-3 PUFA ratios of 3:1 and 5:1 had a higher average daily gain (ADG) and average daily feed intake (ADFI) (P < 0.05) and a lower feed conversion ratio (FCR) (P < 0.05). The concentrations of triglyceride (TG) and total cholesterol (TC) of pigs fed diets with n-6:n-3 PUFA ratios of 1:1 to 5:1 decreased (P < 0·05), whereas the ratio of concentrations of high-density to low-density lipoprotein cholesterol (HDL-C:LDL-C) in pigs fed a diet with an n-6:n-3 PUFA ratio of 2:1 increased (P < 0·05) compared with those fed a diet with an n-6:n-3 PUFA ratio of 8:1. Pigs fed a diet with n-6:n-3 PUFA ratios of 2:1 and 3:1 had lower concentrations of leptin (LEP) and interleukin 6 (IL-6) (P < 0·05), and higher concentrations of adiponectin (APN) (P < 0·05) than those fed diets with n-6:n-3 PUFA ratios of 5:1 and 8:1. Diets with n-6:n-3 PUFA ratios of 2:1 and 3:1 markedly down-regulated the expression levels of peroxisome proliferator-activated receptor-γ (PPARγ) and lipoprotein lipase (LPL) in both the longissimus dorsi muscle (LM) and subcutaneous adipose tissue (SCAT), but up-regulated the expression level of adipocyte fatty acid binding protein (aP2) in the LM. The expression levels of aP2 and hormone- sensitive lipase were similar to those of PPARγ in the SCAT. Conclusions This study demonstrated that dietary n-6:n-3 PUFA ratios between 3:1 and 5:1 beneficially affected growth performance, optimize blood lipid and adipocytokine levels, enhanced absorption and deposition of n-3 PUFA and regulated lipid metabolism-related genes.

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