Trps1 targets Ccnd1 to regulate mouse Leydig cell proliferation

Andrology ◽  
2021 ◽  
Author(s):  
Jiandong Sun ◽  
Hua Li ◽  
Chengyu Lv ◽  
Eman Draz ◽  
Yue Liu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Leydig Cell ◽  
Mouse Leydig Cell

Role of protein kinase C in the regulation of steroidogenesis in the mouse Leydig cell

1986 ◽  
Vol 113 (1_Suppl) ◽  
pp. S63-S64
Author(s):  
A. K. MUKHOPADHYAY ◽  
H. G. BOHNET
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Leydig Cell ◽  
Kinase C ◽  
Mouse Leydig Cell

AN IMPROVED IN VITRO BIOASSAY METHOD FOR MEASURING LUTEINIZING HORMONE (LH) ACTIVITY USING MOUSE LEYDIG CELL PREPARATIONS

1974 ◽  
Vol 77 (4) ◽  
pp. 655-671 ◽  
Author(s):  
M.-P. Van Damme ◽  
D. M. Robertson ◽  
E. Diczfalusy
Keyword(s):  
Luteinizing Hormone ◽  
Leydig Cell ◽  
Marked Reduction ◽  
Improved Method ◽  
In Vitro Bioassay ◽  
Bioassay Method ◽  
Adult Mice ◽  
Assay Design ◽  
Mouse Leydig Cell

ABSTRACT An improved in vitro bioassay method for the measurement of LH activity is presented. The method is based on the assay of testosterone produced by "Leydig cell" preparations from mouse testes in the presence of added gonadotrophin. The method is significantly improved in terms of sensitivity, precision and practicability when compared to the previously described bioassay method employing decapsulated testes from adult mice. The sensitivity of the improved method is 15 μIU for HCG and 50 μIU for HMG. The useful range of the method is 15–260 μIU for HCG and 50–900 μIU for HMG. Using a 3 + 3 point assay design with each dose in quadruplicate, a mean index of precision (λ̅) of 0.044 was obtained in 19 assays. Human FSH, TSH, ACTH, LTH, STH, oxytocin, vasopressin and LHRH preparations did not influence the bioassay method at levels likely to be found in biological samples. A good correlation was found between estimates obtained by the "Leydig cell" method and by the method using decapsulated testes when various HCG and HMG preparations were used. With the proposed method at least 30 samples can be assayed each week by 2 persons, with a marked reduction in cost.

Estrogen induces secretary proteins in transformed mouse Leydig cell in vivo, but not in vitro

1984 ◽  
Vol 124 (1) ◽  
pp. 277-282 ◽  
Author(s):  
Bunzo Sato ◽  
Yasuko Nishizawa ◽  
Makoto Nakao ◽  
Keizo Noma ◽  
Susumu Kishimoto ◽  
...  
Keyword(s):  
Leydig Cell ◽  
Mouse Leydig Cell

scholarly journals Screening of the Active Component Promoting Leydig Cell Proliferation from Lepidium meyenii Using HPLC-ESI-MS/MS Coupled with Multivariate Statistical Analysis

Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2101 ◽  
Author(s):  
Xiao-chen Gao ◽  
Jing-wei Lv ◽  
Chun-nan Li ◽  
Nan-xi Zhang ◽  
Lin-lin Tian ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Leydig Cell ◽  
Active Sites ◽  
Docking Simulation ◽  
Cyclophilin D ◽  
Quick Method ◽  
Specific Chemical ◽  
Hplc Fingerprint ◽  
Lepidium Meyenii ◽  
Testosterone Secretion

Lepidium meyenii is now widely consumed as a functional food and medicinal product, which is known as an enhancer of reproductive health. However, the specific chemical composition and mechanism of action for improving sexual function are unclear. The present study aims at screening and determining the potential compounds, which promote mouse leydig cells (TM3) proliferation. The partial least squares analysis (PLS) was employed to reveal the correlation between common peaks of high performance liquid chromatography (HPLC) fingerprint of L. meyenii and the proliferation activity of TM3. The results suggested that three compounds had good activities on the proliferation of TM3 and promoting testosterone secretion, there were N-benzyl-hexadecanamide, N-benzyl-(9z,12z)-octadecadienamide and N-benzyl-(9z,12z,15z)-octadecatrienamide which might be the potential bioactive markers related to the enhancing sexual ability functions of L. meyenii. The first step in testosterone synthesis is the transport of cholesterol into the mitochondria, and the homeostasis of mitochondrial function is related to cyclophilin D (CypD). In order to expound how bioactive ingredients lead to promoting testosterone secretion, a molecular docking simulation was used for further illustration in the active sites and binding degree of the ligands on CypD. The results indicated there was a positive correlation between the binding energy absolute value and testosterone secretion activity. In addition, in this study it also provided the reference for a simple, quick method to screen the promoting leydig cell proliferation active components in traditional Chinese medicine (TCM).

Estrogen Binding Component of Mouse Leydig Cell Tumor: Anin VitroConversion from Nonreceptor to Receptor-Like Molecule*

Endocrinology ◽  
1981 ◽  
Vol 108 (2) ◽  
pp. 612-619 ◽  
Author(s):  
BUNZO SATO ◽  
YOSHIAKI MAEDA ◽  
KEIZO NOMA ◽  
KEISHI MATSUMOTO ◽  
YUICHI YAMAMURA
Keyword(s):  
Leydig Cell ◽  
Leydig Cell Tumor ◽  
Cell Tumor ◽  
Estrogen Binding ◽  
Mouse Leydig Cell ◽  
Binding Component
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