Icariin modulates eNOS activity via effect on post‐translational protein‐protein interactions to improve erectile function of spontaneously hypertensive rats

Andrology ◽  
2020 ◽  
Author(s):  
Qin‐Wen Liu ◽  
Zhi‐Hui Yang ◽  
Jun Jiang ◽  
Rui Jiang
Keyword(s):  
Protein Interactions ◽  
Erectile Function ◽  
Spontaneously Hypertensive Rats ◽  
Protein Protein Interactions ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Enos Activity

Quercetin downregulates NADPH oxidase, increases eNOS activity and prevents endothelial dysfunction in spontaneously hypertensive rats

2006 ◽  
Vol 24 (1) ◽  
pp. 75-84 ◽  
Author(s):  
Manuel Sánchez ◽  
Milagros Galisteo ◽  
Rocío Vera ◽  
Inmaculada C Villar ◽  
Antonio Zarzuelo ◽  
...  
Keyword(s):  
Endothelial Dysfunction ◽  
Nadph Oxidase ◽  
Spontaneously Hypertensive Rats ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Enos Activity

Chronic administration of genistein improves endothelial dysfunction in spontaneously hypertensive rats: involvement of eNOS, caveolin and calmodulin expression and NADPH oxidase activity

2007 ◽  
Vol 112 (3) ◽  
pp. 183-191 ◽  
Author(s):  
Rocio Vera ◽  
Manuel Sánchez ◽  
Milagros Galisteo ◽  
Inmaculada Concepcion Villar ◽  
Rosario Jimenez ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Endothelial Dysfunction ◽  
Spontaneously Hypertensive Rats ◽  
Chronic Administration ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Caveolin 1 ◽  
Enos Activity ◽  
Wistar Kyoto ◽  
O2 Production

The soya-derived phytoestrogen genistein has been suggested to be protective in cardiovascular diseases. In the present study, we have analysed whether chronic oral genistein might influence endothelial function in male SHRs (spontaneously hypertensive rats) via ERs (oestrogen receptors), changes in eNOS (endothelial NO synthase) activity and vascular O2− (superoxide) production. Rats (23-weeks old) were divided into the following groups: WKY (Wistar–Kyoto)-vehicle, SHR-vehicle, WKY-genistein (10 mg·kg−1 of body weight·day−1); SHR-genistein; SHR-genistein-faslodex (ICI 182780; 2.5 mg·kg−1 of body weight·day−1). Vascular expression of eNOS, caveolin-1 and calmodulin-1 were analysed by Western blotting, eNOS activity by conversion of [3H]arginine into L-[3H]citrulline and O2− production by chemoluminescence of lucigenin. In SHRs, after 5 weeks of treatment, genistein reduced systolic blood pressure and enhanced endothelium-dependent aortic relaxation to acetylcholine, but had no effect on the vasodilator responses to sodium nitroprusside. Compared with WKY rats, SHRs had up-regulated eNOS and down-regulated caveolin-1 and calmodulin-1 expression, increased NADPH-induced O2− production, but reduced eNOS activity. Genistein increased aortic calmodulin-1 protein abundance and eNOS activity, and reduced NADPH-induced O2− production in SHRs. The pure ERα and ERβ antagonist faslodex did not modify any of the changes induced by genistein in SHRs, suggesting that these effects are unrelated to ER stimulation. In conclusion, genistein reduced the elevated blood pressure and endothelial dysfunction in SHRs. This latter effect appears to be related to increased eNOS activity associated with increased calmodulin-1 expression and decreased O2− generation.

Improving Erectile Function of Spontaneously Hypertensive Rats by Silencing ROCK2

Urology ◽  
2014 ◽  
Vol 84 (4) ◽  
pp. 983.e11-983.e18 ◽  
Author(s):  
Xiubo Zhu ◽  
Haocheng Lin ◽  
Rui Jiang ◽  
Run Wang ◽  
Jun Jiang ◽  
...  
Keyword(s):  
Erectile Function ◽  
Spontaneously Hypertensive Rats ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive

Icariin Combined with Breviscapine Improves the Erectile Function of Spontaneously Hypertensive Rats

2014 ◽  
Vol 11 (9) ◽  
pp. 2143-2152 ◽  
Author(s):  
Yongxian Li ◽  
Jun Jiang ◽  
Yanzheng He ◽  
Rui Jiang ◽  
Junxiang Liu ◽  
...  
Keyword(s):  
Erectile Function ◽  
Spontaneously Hypertensive Rats ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive
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