scholarly journals Fibroblast growth factor 2 (FGF2) is present in human spermatozoa and is related with sperm motility. The use of recombinant FGF2 to improve motile sperm recovery

Andrology ◽  
2017 ◽  
Vol 5 (5) ◽  
pp. 990-998 ◽  
Author(s):  
D. J. Garbarino Azúa ◽  
L. Saucedo ◽  
S. Giordana ◽  
M. L. Magri ◽  
M. G. Buffone ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Sperm Motility ◽  
Human Spermatozoa ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Motile Sperm ◽  
Sperm Recovery

scholarly journals Involvement of fibroblast growth factor 2 (FGF2) and its receptors in the regulation of mouse sperm physiology

Reproduction ◽  
2018 ◽  
Vol 156 (2) ◽  
pp. 163-172 ◽  
Author(s):  
Lucía Saucedo ◽  
Cristian Sobarzo ◽  
Nicolás G Brukman ◽  
Héctor A Guidobaldi ◽  
Livia Lustig ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Sperm Motility ◽  
Cellular Proliferation ◽  
Reproductive Tract ◽  
Female Reproductive Tract ◽  
Fibroblast Growth Factor 2 ◽  
Sperm Function ◽  
Fibroblast Growth ◽  
Mouse Sperm

Fibroblast growth factor 2 (FGF2) and its receptors (FGFRs) have been described in several tissues, where they regulate cellular proliferation, differentiation, motility and apoptosis. Although FGF2/FGFRs expression in the male reproductive tract has been reported, there is scarce evidence on their presence in the female reproductive tract and their involvement in the modulation of sperm function. Therefore, the objective of this study was to determine the expression of FGF2 in the female reproductive tract and to assess the role of the FGF2/FGFRs system in the regulation of sperm physiology using the murine model. FGF2 was detected in uterus and oviduct protein extracts, and it was immunolocalized in epithelial cells of the uterus,isthmusandampulla, as well as in thecumulus oophorus-oocyte complex. The receptors FGFR1, FGFR2, FGFR3 and FGFR4 were immunodetected in the flagellum and acrosomal region of sperm recovered from thecaudaepididymis. Analysis of testis sections showed the expression of FGFRs in germ cells at different stages of the spermatogenesis, suggesting the testicular origin of the sperm FGFRs. Sperm incubation with recombinant FGF2 (rFGF2) led to increased sperm motility and velocity and to enhanced intracellular Ca2+levels and acrosomal loss compared to the control. In conclusion, this study shows that FGF2 is expressed in tissues of the female reproductive tract. Also, the fact that functional FGFRs are present in mouse sperm and that rFGF2 affects sperm motility and acrosomal exocytosis, suggests the involvement of this system in thein vivoregulation of sperm function.

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