Monitoring the reactive oxygen species in spermatozoa during liquid storage of boar semen and its correlation with sperm motility, free thiol content and seasonality

Andrologia ◽  
2021 ◽  
Author(s):  
Hoang Xuan Khoi ◽  
Kenji Shimizu ◽  
Yoshitaka Yoneda ◽  
Itaru Minagawa ◽  
Yasuyuki Abe ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Sperm Motility ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Free Thiol ◽  
Liquid Storage ◽  
Thiol Content ◽  
Boar Semen

Effect of endotoxin-induced reactive oxygen species on sperm motility

2001 ◽  
Vol 76 (1) ◽  
pp. 163-166 ◽  
Author(s):  
Kenichiro Urata ◽  
Hisashi Narahara ◽  
Yuichiro Tanaka ◽  
Toru Egashira ◽  
Fusako Takayama ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Sperm Motility ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals Genetic Association in the Maintenance of the Mitochondrial Microenvironment and Sperm Capacity

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Hwang I. S. Thomas ◽  
Ying-Shiuan Chen ◽  
Ching-Han Hung ◽  
Dilip Bhargava Sreerangaraja Urs ◽  
Tien-Ling Liao ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Sperm Motility ◽  
Manganese Superoxide Dismutase ◽  
Uncoupling Protein ◽  
Reactive Oxygen ◽  
Human Sperm ◽  
Oxygen Species ◽  
Genotype Frequencies ◽  
The Impact

Sperm motility is one of the major determinants of male fertility. Since sperm need a great deal of energy to support their fast movement by active metabolism, they are thus extremely vulnerable to oxidative damage by the reactive oxygen species (ROS) and other free radicals generated as byproducts in the electron transport chain. The present study is aimed at understanding the impact of a mitochondrial oxidizing/reducing microenvironment in the etiopathology of male infertility. We detected the mitochondrial DNA (mtDNA) 4,977 bp deletion in human sperm. We examined the gene mutation of ATP synthase 6 (ATPase6 m.T8993G) in ATP generation, the gene polymorphisms of uncoupling protein 2 (UCP2, G-866A) in the uncoupling of oxidative phosphorylation, the role of genes such as manganese superoxide dismutase (MnSOD, C47T) and catalase (CAT, C-262T) in the scavenging system in neutralizing reactive oxygen species, and the role of human 8-oxoguanine DNA glycosylase (hOGG1, C1245G) in 8-hydroxy-2 ′ -deoxyguanosine (8-OHdG) repair. We found that the sperm with higher motility were found to have a higher mitochondrial membrane potential and mitochondrial bioenergetics. The genotype frequencies of UCP2 G-866A, MnSOD C47T, and CAT C-262T were found to be significantly different among the fertile subjects, the infertile subjects with more than 50% motility, and the infertile subjects with less than 50% motility. A higher prevalence of the mtDNA 4,977 bp deletion was found in the subjects with impaired sperm motility and fertility. Furthermore, we found that there were significant differences between the occurrences of the mtDNA 4,977 bp deletion and MnSOD (C47T) and hOGG1 (C1245G). In conclusion, the maintenance of the mitochondrial redox microenvironment and genome integrity is an important issue in sperm motility and fertility.

315 REACTIVE OXYGEN SPECIES AND OXIDATIVE STRESS IN CANINE SEMEN FRACTIONS

2010 ◽  
Vol 22 (1) ◽  
pp. 313
Author(s):  
C. F. Lucio ◽  
M. Nichi ◽  
F. M. Regazzi ◽  
T. F. Rück ◽  
L. C. G. Silva ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Free Radicals ◽  
Sperm Motility ◽  
Seminal Plasma ◽  
Pearson Correlation ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Low Concentrations ◽  
Viable Sperm

Reactive oxygen species (ROS) are physiologically produced by spermatozoa and leukocytes present in the seminal plasma. In low concentrations, ROS play an important role in sperm function because they are required for sperm fertilizing capacity, mainly during sperm capacitation and hyperactivation (Saleh RA and Agarwal A 2002 J. Androl. 23, 737-752). However, an imbalance between the formation of free radicals and the capacity for defense of the antioxidant mechanisms may lead to cell damage (Rover Jr L et al. 2001 Química Nova 24, 112-119). Spermatozoa are sensitive to lipid peroxidation due to its high content of polyunsaturated fatty acids and low concentrations of protective enzymes (Sharma RK and Agarwa LA 1996 Urology 48, 835-850). The aims of the present study were to compare ROS content among the 3 fractions of canine semen and to correlate these values with sperm variables. Semen samples were collected from 15 healthy dogs of distinct breeds aged 2 to 6 years. Sperm analysis was performed through motility, forward progressive velocity, morphology, and the percentage of viable sperm with the use of the eosin/nigrosin stain. The determination of thiobarbituric acid reactive substances (TBARS) was used to estimate the degree of lipid peroxidation in each sperm fraction. Values were compared using ANOVA and Tukey’s test for multiple comparisons at a significance level of 5%. Pearson correlation was used to calculate the relationship between sperm variables in the second fraction. Sperm motility, velocity, and percentage of viable sperm were within the normal range for canine semen: 84±2%, 3.4±0.1%, and 83 ± 2%, respectively. The sperm-rich fraction presented statistically higher concentrations of TBARS (1474.19 ± 245.78 ng mL-1) compared to the first and third fractions (579.41 ± 171.23 and 399.62 ± 58.08 ng mL-1, respectively; P < 0.05), indicating that spermatozoa and epididymal secretions are the main source of free radicals. No statistical correlation among TBARS and sperm motility and velocity were verified. However, a positive correlation was observed between the percentage of sperm proximal droplets and TBARS (r = 0.44, P = 0.7). This result suggests that a high incidence of sperm proximal droplets can enhance ROS formation in seminal plasma. Hence, canine sperm presenting delayed maturation in the epididymes produce higher concentrations of free radicals. In fact, sperm production of ROS occurs mainly by abnormal cells, especially the ones containing cytoplasm residues (Gomez E et al. 1998 Int. J. Androl. 21, 81-94). However, no protective effect of the sperm distal droplets was verified in canine semen as observed elsewhere for the bovine spermatozoa (Nichi M et al. 2007 Theriogenology 67, 334-340). In conclusion, spermatozoa and epididymal fluids are the primordial source of free radicals in canine seminal plasma, mainly when sperm proximal droplets are present.

Negative correlation between presence of reactive oxygen species and Sperm Motility Index in whole semen samples of infertile males

2017 ◽  
Vol 15 (3) ◽  
pp. 84-89
Author(s):  
Shinnosuke Kuroda ◽  
Yasushi Yumura ◽  
Kohei Mori ◽  
Kengo Yasuda ◽  
Teppei Takeshima ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Sperm Motility ◽  
Negative Correlation ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Motility Index
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