Zinc is a strong stimulant of metallothionein synthesis in the ischaemic testis tissue

Andrologia ◽  
2021 ◽  
Author(s):  
Betul Yazğan ◽  
Abdulkerim Kasim Baltaci ◽  
Rasim Mogulkoc ◽  
Mustafa Cihat Avunduk
Keyword(s):  
Testis Tissue

Cloning and Sequence Analysis of Cold Inducible RNA-binding Protein cDNA from Testis Tissue in BALB/C Mice*

2010 ◽  
Vol 2009 (1) ◽  
pp. 87-90
Author(s):  
Fuhou JIN ◽  
Yan PANG ◽  
Shize LI ◽  
Huanmin YANG ◽  
Hong JI ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Testis Tissue

scholarly journals The Effect of High-fat Diet and Exercise on KISS-1/GPR54 Expression in Testis of Growing Rats

2020 ◽  
Author(s):  
Junpeng Feng ◽  
Rui Xu ◽  
Yafei Li ◽  
Qishu Zhou ◽  
Ge Song ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Exercise Intervention ◽  
Expression Level ◽  
Testicular Development ◽  
High Fat ◽  
Growing Period ◽  
Diet And Exercise ◽  
Mrna Content ◽  
G Protein Coupled ◽  
Testis Tissue

Abstract Purpose: To find the expression of KISS-1 and G protein-coupled receptor 54 in rats testis from PND 21st to 56th. Method: 128 three-week-old weaned rats underwent high-fat diet and exercise (60%–70% VO2max, 1 h/day, 5 days/week) intervention and were randomly divided into group C, CE, HC, or HE. Sample time points were set on the PND 21st, 35th, 43rd, and 56th. The testicular testosterone and the mRNA content, and protein content of KISS-1 and GPR54 in testis tissue were detected by ELISA, RT-qPCR, and Western blotting. Result: (1) The protein of KISS-1 and GPR54 increased gradually during the growing period. KISS-1 mRNA peaked at 35D and GPR54 peaked at 43D. (2) High-fat diet affected the expression of the KISS-1/GPR54 system in rat testis and reduced the expression level of KISS-1 protein. (3) 60%–70% VO2max exercise decreased the KISS-1/GPR54 expression level. Exercise intervention improved testicular development in rats with a high-fat diet.Conclusion: The expression of KISS-1/GPR54 increased during the growing period. High-fat diet can downregulate the protein and gene expression of KISS-1/GPR54 and change the expression trend. 60%–70% VO2max exercise decreased the expression of KISS-1/GPR54, which may be involved in the effects of exercise on high-fat dietary sex hormone disorders.

scholarly journals Comparative transcriptome and histomorphology analysis of testis tissues from mulard and Pekin ducks

2020 ◽  
Vol 63 (2) ◽  
pp. 303-313
Author(s):  
Li Li ◽  
Linli Zhang ◽  
Zhenghong Zhang ◽  
Nemat O. Keyhani ◽  
Qingwu Xin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Differential Expression ◽  
Candidate Genes ◽  
Transcriptome Sequencing ◽  
Differentially Expressed ◽  
Pekin Duck ◽  
Comparative Transcriptome ◽  
Qrt Pcr ◽  
Pekin Ducks ◽  
Testis Tissue

Abstract. Testicular transcriptomes were analyzed to characterize the differentially expressed genes between mulard and Pekin ducks, which will help establish gene expression datasets to assist in further determination of the mechanisms of genetic sterility in mulard ducks. Paraffin sections were made to compare the developmental differences in testis tissue between mulard and Pekin ducks. Comparative transcriptome sequencing of testis tissues was performed, and the expression of candidate genes was verified by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). In mulard ducks, spermatogonia and spermatocytes were arranged in a disordered manner, and no mature sperm were observed in the testis tissue. However, different stages of development of sperm were observed in seminiferous tubules in the testis tissue of Pekin ducks. A total of 43.84 Gb of clean reads were assembled into 193 535 UniGenes. Of these, 2131 transcripts exhibited differential expression (false discover rate <0.001 and fold change ≥2), including 997 upregulated and 1134 downregulated transcripts in mulard ducks as compared to those in Pekin duck testis tissues. Several upregulated genes were related to reproductive functions, including ryanodine receptor 2 (RYR2), calmodulin (CALM), argininosuccinate synthase and delta-1-pyrroline-5-carboxylate synthetase ALDH18A1 (P5CS). Downregulated transcripts included the testis-specific serine/threonine-protein kinase 3, aquaporin-7 (AQP7) and glycerol kinase GlpK (GK). The 10 related transcripts involved in the developmental biological process were identified by GO (Gene Ontology) annotation. The KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways indicated that peroxisome proliferator-activated receptors (PPARs) and calcium signaling pathways were significantly (P<0.001) associated with normal testis physiology. The differential expression of select genes implicated in reproductive processes was verified by qRT-PCR, which was consistent with the expression trend of transcriptome sequencing (RNA-seq). Differentially expressed candidate genes RYR2, CALM, P5CS, AQP7 and GK were identified by transcriptional analysis in mulard and Pekin duck testes. These were important for the normal development of the male duck reproductive system. These data provide a framework for the further exploration of the molecular and genetic mechanisms of sterility in mulard ducks. Highlights. The mulard duck is an intergeneric sterile hybrid offspring resulting from mating between Muscovy and Pekin ducks. The transcriptomes of testis tissue from mulard and Pekin ducks were systematically characterized, and differentially expressed genes were screened, in order to gain insights into potential gonad gene expression mechanisms contributing to genetic sterility in mulard ducks.

scholarly journals Induction of meiosis in fetal mouse testis in vitro by rete testis tissue from pubertal mice and bulls

Reproduction ◽  
1979 ◽  
Vol 56 (2) ◽  
pp. 653-656 ◽  
Author(s):  
J. Grinsted ◽  
A. G. Byskov ◽  
M. P. Andreasen
Keyword(s):  
Rete Testis ◽  
Mouse Testis ◽  
Fetal Mouse ◽  
Testis Tissue

MP66-18 DECELLULARIZED WHOLE TESTIS AS A POTENTIAL BIOSCAFFOLD FOR TESTIS TISSUE-ENGINEERING

2017 ◽  
Vol 197 (4S) ◽  
Author(s):  
Aram AKBARZADEH ◽  
Maral KIANIMANESH ◽  
Maryam EBADI ◽  
Ahmad MASOOMI ◽  
Fereshteh GHAZISAEEDI ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Testis Tissue
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