The effect of vitamin E on sperm motility and viability in asthenoteratozoospermic men: In vitro study

Andrologia ◽  
2020 ◽  
Author(s):  
Ali Asghar Ghafarizadeh ◽  
Mahdi Malmir ◽  
Samira Naderi Noreini ◽  
Tayebeh Faraji ◽  
Zeynab Ebrahimi
Keyword(s):  
Vitamin E ◽  
Sperm Motility ◽  
In Vitro Study ◽  
Vitro Study

Hepatotoxic effect of ochratoxin A and citrinin, alone and in combination, and protective effect of vitamin E: In vitro study in HepG2 cell

2015 ◽  
Vol 83 ◽  
pp. 151-163 ◽  
Author(s):  
Loganathan Gayathri ◽  
Rajakumar Dhivya ◽  
Dharumadurai Dhanasekaran ◽  
Vaiyapuri S. Periasamy ◽  
Ali A. Alshatwi ◽  
...  
Keyword(s):  
Vitamin E ◽  
Protective Effect ◽  
Ochratoxin A ◽  
Hepg2 Cell ◽  
In Vitro Study ◽  
Vitro Study ◽  
Hepatotoxic Effect

scholarly journals Bacterial adherence on UHMWPE with vitamin E: an in vitro study

2011 ◽  
Vol 22 (7) ◽  
pp. 1701-1706 ◽  
Author(s):  
E. Gómez-Barrena ◽  
J. Esteban ◽  
D. Molina-Manso ◽  
H. Adames ◽  
M. J. Martínez-Morlanes ◽  
...  
Keyword(s):  
Vitamin E ◽  
In Vitro Study ◽  
Bacterial Adherence ◽  
Vitro Study

scholarly journals Polyblend Nanofibers to Regenerate Gingival Tissue: A Preliminary In Vitro Study

2021 ◽  
Vol 8 ◽  
Author(s):  
Elena Canciani ◽  
Nicoletta Gagliano ◽  
Francesca Paino ◽  
Evžen Amler ◽  
Radek Divin ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Vitamin E ◽  
Human Fibroblasts ◽  
In Vitro Study ◽  
Vitro Study ◽  
Gingival Tissue ◽  
Collagen Deposition ◽  
Dental Practitioners ◽  
In Cells

Aim: The regeneration of small periodontal defects has been considered an important divide and challenging issue for dental practitioners. The aim of this preliminary in vitro study was to analyze the effects of polycaprolactone (PCL) nanofibers enriched with hyaluronic acid and vitamin E vs. nude nanofibers on gingival fibroblasts activity, an innovative graft for periodontal soft tissue regeneration purposes.Methods: Nanofibers were produced in PCL (NF) or PCL enriched with hyaluronic acid and vitamin E (NFE) by electrospinning technique. NF and NFE were stereologically and morphologically characterized by scanning electron microscope (SEM), and composition was analyzed by infrared spectroscopy. Human fibroblasts were obtained from one gingival tissue fragment (HGF) and then seeded on NF, NFE, and plastic (CT). Cell adhesion and morphology were evaluated using SEM at 24 h and cell viability after 24, 48, and 72 h by alamarBlue® assay. Gene expression for COL-I, LH2b, TIMP-1, PAX, and VNC was analyzed by real-time RT-PCR in samples run in triplicate and GAPDH was used as housekeeping gene. Slot blot analysis was performed and immunoreactive bands were revealed for MMP-1 and COL-I. YAP and p-YAP were analyzed by Western blot and membranes were reprobed by α-tubulin. Statistical analysis was performed.Results: IR spectrum revealed the presence of PCL in NF and PCL and vitamin E and hyaluronic acid in NFE. At 24 h, HGF adhered on NF and NFE conserving fibroblast like morphology. At 72 h from seeding, statistically significant differences were found in proliferation of HGF cultured on NF compared to NFE. Expression of genes (LH2b, TIMP-1, and MMP-1) and proteins (COL-I) related to collagen turnover revealed a reduction of COL-1 secretion in cells cultured on NF and NFE compared to CT; however, NFE stimulated cross-linked collagen deposition. Mechanosensor genes (PAX, VNC, and YAP) were upregulated in HGF on NF while they were decreased in cells grown on NFE.Conclusion: Preliminary data suggest that PCL-enriched nanofibers could represent a support to induce HGF proliferation, adhesion, collagen cross-linking, and to reduce collagen degradation, therefore favoring collagen deposition in gingival connective tissue.

scholarly journals Effect of phosphodiesterase type 5 inhibitors on sperm motility and acrosome reaction: An in vitro study

2021 ◽  
Vol 62 ◽  
Author(s):  
Seung-Hun Song ◽  
Dong Hyuk Shin ◽  
Young Sun Her ◽  
Mi Hee Oh ◽  
Jung Won Baek ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Acrosome Reaction ◽  
In Vitro Study ◽  
Vitro Study ◽  
Phosphodiesterase Type 5 Inhibitors ◽  
Phosphodiesterase Type

Silymarin and protein kinase A inhibitor modulate glucose-mediated mouse sperm motility: An in vitro study

2015 ◽  
Vol 15 (3) ◽  
pp. 172-177 ◽  
Author(s):  
Yi-Chuan Chen ◽  
Li-Chern Pan ◽  
Cheng-Wei Lai ◽  
Ying-Shan Chien ◽  
Tzu-Hua Wu
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Sperm Motility ◽  
In Vitro Study ◽  
Vitro Study ◽  
Mouse Sperm ◽  
Kinase A

Effects of Electromagnetic Radiation from a Cellular Phone on Human Sperm Motility: An In Vitro Study

2006 ◽  
Vol 37 (7) ◽  
pp. 840-843 ◽  
Author(s):  
Osman Erogul ◽  
Emin Oztas ◽  
Ibrahim Yildirim ◽  
Tayfun Kir ◽  
Emin Aydur ◽  
...  
Keyword(s):  
Electromagnetic Radiation ◽  
Sperm Motility ◽  
In Vitro Study ◽  
Cellular Phone ◽  
Human Sperm ◽  
Vitro Study
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