Omeprazole does not alter human sperm motility, viability or DNA integrity in vitro

Andrologia ◽  
2019 ◽  
Vol 51 (6) ◽  
pp. e13260 ◽  
Author(s):  
Saleem Ali Banihani ◽  
Alayna'‐almarddyah Abdullah Al‐khawalde
Keyword(s):  
Sperm Motility ◽  
Human Sperm ◽  
Dna Integrity

scholarly journals Protective Effect of Chlorogenic Acid on Human Sperm: In Vitro Studies and Frozen—Thawed Protocol

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 744
Author(s):  
Daria Noto ◽  
Giulia Collodel ◽  
Daniela Cerretani ◽  
Cinzia Signorini ◽  
Laura Gambera ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Sperm Motility ◽  
Plasma Membranes ◽  
Culture Media ◽  
Human Sperm ◽  
Dna Integrity ◽  
H2o2 Treatment ◽  
Transmission Electron ◽  
The Media

The study evaluated the chlorogenic acid (CGA) antioxidant potential on oxidative stress (OS) induced in vitro in human spermatozoa and during cryopreservation procedure. Swim-up selected spermatozoa were treated with 100 µM CGA, 100 µM H2O2 to induce lipid peroxidation (LPO), and with both compounds and the effects on mitochondrial membrane potential (MMP) by JC-1, DNA integrity by acridine orange (AO), and sperm ultrastructure by transmission electron microscopy (TEM), were evaluated. CGA antioxidant activity was assessed by measuring malondialdehyde (MDA) and F2-isoprostanes (F2-IsoPs) in the media. The CGA protective activity and the immunolocalization of Phospho-AMPKα (Thr172) were explored in frozen-thawed sperm. CGA was not toxic for sperm motility, DNA integrity and MMP. The increase in MDA (p < 0.05) and F2-IsoPs (p < 0.001), DNA damage (p < 0.01) and low MMP (p < 0.01) levels after H2O2 treatment were reduced in presence of CGA as well as the percentage of broken plasma membranes (p < 0.01) and altered acrosomes (p < 0.01) detected by TEM. Treated frozen-thawed spermatozoa showed increased sperm motility (p < 0.01), DNA integrity (p < 0.01), MMP (p < 0.01), reduced MDA (p < 0.01) and increased sperm percentage with Phospho-AMPKα labelling in the head (p < 0.001). CGA can be used to supplement culture media during semen handling and cryopreservation where OS is exacerbated.

scholarly journals Cytotoxic Effects of Benzene Metabolites on Human Sperm Function: An In Vitro Study

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Priyanka Mandani ◽  
Ketki Desai ◽  
Hyacinth Highland
Keyword(s):  
Sperm Motility ◽  
Nuclear Dna ◽  
In Vitro Study ◽  
Human Sperm ◽  
Dna Integrity ◽  
Sperm Function ◽  
Cytotoxic Effects ◽  
Membrane Function ◽  
Atp Production

In recent years, individuals are rampantly exposed to vapours of benzene, through paint, plastic, petroleum industries, fuel exhaust, and tobacco smoke. Hence the present investigation was directed towards determining the effect of benzene metabolites, namely, phenol-hydroquinone and catechol, on the motility, viability, and nuclear integrity of the human spermatozoa. From the results obtained it was clear that exposure to phenol-hydroquinone caused a significant decline in both, sperm motility and viability. Exposure to a phenol-hydroquinone (Phase I) microenvironment may therefore inhibit metabolically active enzymes, thus impeding ATP production, and in turn lowers sperm motility and viability. In addition, the present study also revealed that both metabolites of benzene caused significant denaturation of sperm nuclear DNA. Hence, exposure to phenol-hydroquinone in vitro could have resulted in generation of free radicals and altered membrane function, which is reflected by a decline in the motility, viability, and loss of sperm nuclear DNA integrity. In Phase II, the exposure of human sperm in vitro to varied concentrations of catechol caused only insignificant changes in sperm motility and viability as compared to those observed on exposure to phenol-hydroquinone. Hence, exposure to catechol appeared to have less toxic effects than those of phenol-hydroquinone.

scholarly journals Current Insights and Latest Updates in Sperm Motility and Associated Applications in Assisted Reproduction

2020 ◽  
Author(s):  
Reyon Dcunha ◽  
Reda S. Hussein ◽  
Hanumappa Ananda ◽  
Sandhya Kumari ◽  
Satish Kumar Adiga ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Reproductive Tract ◽  
Management Strategies ◽  
Human Sperm ◽  
Pharmacological Agents ◽  
Factors Affecting ◽  
Global Trend ◽  
Increased Risk

AbstractSpermatozoon is a motile cell with a special ability to travel through the woman’s reproductive tract and fertilize an oocyte. To reach and penetrate the oocyte, spermatozoa should possess progressive motility. Therefore, motility is an important parameter during both natural and assisted conception. The global trend of progressive reduction in the number and motility of healthy spermatozoa in the ejaculate is associated with increased risk of infertility. Therefore, developing approaches for maintaining or enhancing human sperm motility has been an important area of investigation. In this review we discuss the physiology of sperm, molecular pathways regulating sperm motility, risk factors affecting sperm motility, and the role of sperm motility in fertility outcomes. In addition, we discuss various pharmacological agents and biomolecules that can enhance sperm motility in vitro and in vivo conditions to improve assisted reproductive technology (ART) outcomes. This article opens dialogs to help toxicologists, clinicians, andrologists, and embryologists in understanding the mechanism of factors influencing sperm motility and various management strategies to improve treatment outcomes.

Alkaloids from Areca (Betel) Nuts and Their Effects on Human Sperm Motility In Vitro

2012 ◽  
Vol 77 (4) ◽  
pp. T70-T78 ◽  
Author(s):  
Jingsong Yuan ◽  
Dajian Yang ◽  
Yonghong Liang ◽  
Wenping Gao ◽  
Zhipeng Ren ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Human Sperm

scholarly journals In-vitro effects of Thymus munbyanus essential oil and thymol on human sperm motility and function

2015 ◽  
Vol 31 (3) ◽  
pp. 411-420 ◽  
Author(s):  
Amirouche Chikhoune ◽  
Laurence Stouvenel ◽  
Mokrane Iguer-Ouada ◽  
Mohamed Hazzit ◽  
Alain Schmitt ◽  
...  
Keyword(s):  
Essential Oil ◽  
Sperm Motility ◽  
Human Sperm ◽  
In Vitro Effects ◽  
And Function

scholarly journals Pentoxifylline stimulates human sperm motility both in vitro and after oral therapy.

1991 ◽  
Vol 31 (6) ◽  
pp. 711-714 ◽  
Author(s):  
MR Shen ◽  
PH Chiang ◽  
RC Yang ◽  
CY Hong ◽  
SS Chen
Keyword(s):  
Sperm Motility ◽  
Oral Therapy ◽  
Human Sperm
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