Transformation, migration and outcome of residual bodies in the seminiferous tubules of the rat testis

Andrologia ◽  
2017 ◽  
Vol 49 (10) ◽  
pp. e12786 ◽  
Author(s):  
C.-Y. Xiao ◽  
Y.-Q. Wang ◽  
J.-H. Li ◽  
G.-C. Tang ◽  
S.-S. Xiao
Keyword(s):  
Seminiferous Tubules ◽  
Rat Testis ◽  
Residual Bodies

Residual Bodies of Seminiferous Tubules of the Rat

Nature ◽  
1959 ◽  
Vol 184 (4682) ◽  
pp. 249-251 ◽  
Author(s):  
B. V. KINGSLEY SMITH ◽  
D. LACY
Keyword(s):  
Seminiferous Tubules ◽  
Residual Bodies

Biochemical Functions of Isolated Interstitial Tissue and Seminiferous Tubules from Rat Testis

1973 ◽  
pp. 533-560
Author(s):  
H.J. van der Molen ◽  
A.O. Brinkmann ◽  
B.A. Cooke ◽  
F.H. de Jong ◽  
E. Mulder ◽  
...  
Keyword(s):  
Seminiferous Tubules ◽  
Interstitial Tissue ◽  
Rat Testis

scholarly journals Glial cell-line-derived neurotropic factor and its receptors are expressed by germinal and somatic cells of the rat testis

2006 ◽  
Vol 190 (1) ◽  
pp. 59-71 ◽  
Author(s):  
Sophie Fouchécourt ◽  
Murielle Godet ◽  
Odile Sabido ◽  
Philippe Durand
Keyword(s):  
Cell Line ◽  
Glial Cell ◽  
Sertoli Cells ◽  
Small Population ◽  
Seminiferous Tubules ◽  
Rat Testis ◽  
Old Rats ◽  
Neurotropic Factor ◽  
Pachytene Spermatocytes ◽  
Factor Α

Glial cell-line-derived neurotropic factor (GDNF) and its receptors glial cell-line-derived neurotropic factor α (GFR1α) and rearranged during transformation (RET) have been localized in the rat testis during postnatal development. The three mRNAs, and GDNF and GFR1α proteins were detected in testis extracts from 1- to 90-day-old rats by reverse transcriptase PCR and Western blotting respectively. The three mRNAs were present in Sertoli cells from 20- and 55-day-old rats, pachytene spermatocytes (PS), and round spermatids (RS). The GDNF and GFR1α proteins were detected in PS, RS, and Sertoli cells. GDNF and GFR1α were also detected using flow cytometry in spermatogonia and preleptotene spermatocytes, and in secondary spermatocytes. The localization of GDNF and GFR1α in germ and Sertoli cells was confirmed by immunocytochemistry. The hypothesis that GDNF may control DNA synthesis of Sertoli cells and/or spermatogonia in the immature rat was addressed using cultures of seminiferous tubules from 7- to 8-day-old rats. Addition of GDNF for 48 h resulted in a twofold decrease in the percentage of spermatogonia able to duplicate DNA, whereas Sertoli cells were not affected. These results are consistent with a role of GDNF in inhibiting the S-phase entrance of a large subset of differentiated type A spermatogonia, together with an enhancing effect of the factor on a small population of undifferentiated (stem cells) spermatogonia. Moreover, the wide temporal and spatial expression of GDNF and its receptors in the rat testis suggest that it might act at several stages of spermatogenesis.

scholarly journals Identification of rat testis galactosyl receptor using antibodies to liver asialoglycoprotein receptor: purification and localization on surfaces of spermatogenic cells and sperm.

1989 ◽  
Vol 108 (2) ◽  
pp. 367-375 ◽  
Author(s):  
M Abdullah ◽  
A L Kierszenbaum
Keyword(s):  
Binding Affinity ◽  
Rat Liver ◽  
Asialoglycoprotein Receptor ◽  
Seminiferous Tubules ◽  
Testicular Development ◽  
Spermatogenic Cells ◽  
Epididymal Sperm ◽  
Rat Testis ◽  
Receptor Purification ◽  
Dorsal Portion

We have found that the rat testis contains a cell surface galactosyl receptor that is antigenically related to the minor species of rat liver asialoglycoprotein receptor (ASGP-r) and has binding affinity for galactose coupled to agarose. In immunoblotting experiments, rat testis galactosyl receptor (RTG-r) is recognized by antiserum raised against the minor ASGP-r species of rat liver (designated rat hepatic lectin-2/3, RHL-2/3). Antiserum raised against the major species RHL-1 does not recognize an antigenic protein equivalent to RTG-r. Triton X-100-extracted rat liver and testes preparations fractionated by affinity chromatography on galactose-agarose and resolved by SDS-PAGE under reducing conditions, show that rat liver contains both the major (RHL-1) and minor (RHL-2/3) ASGP-r species whereas rat testis displays only a receptor species comigrating with RHL-2/3. RTG-r was present throughout testicular development. The receptor was found in seminiferous tubules, cultured Sertoli and spermatogenic cells, and epididymal sperm. Indirect immunofluorescent studies show RHL-2/3-like immunoreactivity on the surface of Sertoli cell, meiotic prophase spermatocytes, spermatids, and epididymal sperm. In spermatids and sperm, the immunoreactivity is restricted to the plasma membrane overlying the dorsal portion of the head. Because of RTG-r has galactose binding affinity, is present on surfaces of Sertoli and developing meiotic and postmeiotic spermatogenic cells, and overlies a region of the intact acrosome on epididymal sperm, RTG-r may have a role in spermatogenesis and in events leading to sperm-egg recognition.

Observations on the binding of androgens by rat testis seminiferous tubules and testis extracts

1974 ◽  
Vol 1 (3) ◽  
pp. 167-187 ◽  
Author(s):  
Richard G. Vernon ◽  
Bozena Kopec ◽  
Irving B. Fritz
Keyword(s):  
Seminiferous Tubules ◽  
Rat Testis

scholarly journals Novel Expression and Direct Effects of Adiponectin in the Rat Testis

Endocrinology ◽  
2008 ◽  
Vol 149 (7) ◽  
pp. 3390-3402 ◽  
Author(s):  
J. E. Caminos ◽  
R. Nogueiras ◽  
F. Gaytán ◽  
R. Pineda ◽  
C. R. González ◽  
...  
Keyword(s):  
Hormonal Regulation ◽  
Ex Vivo ◽  
Seminiferous Tubules ◽  
Peroxisome Proliferator ◽  
Direct Effects ◽  
Peroxisome Proliferator Activated Receptor ◽  
Rat Testis ◽  
Protein Levels ◽  
Endocrine Organs

Adiponectin is an adipocyte hormone, with relevant roles in lipid metabolism and glucose homeostasis, recently involved in the control of different endocrine organs, such as the placenta, pituitary and, likely, the ovary. However, whether as described previously for other adipokines, such as leptin and resistin, adiponectin is expressed and/or conducts biological actions in the male gonad remains unexplored. In this study, we provide compelling evidence for the expression, putative hormonal regulation, and direct effects of adiponectin in the rat testis. Testicular expression of adiponectin was demonstrated along postnatal development, with a distinctive pattern of RNA transcripts and discernible protein levels that appeared mostly located at interstitial Leydig cells. Testicular levels of adiponectin mRNA were marginally regulated by pituitary gonadotropins but overtly modulated by metabolic signals, such as glucocorticoids, thyroxine, and peroxisome proliferator-activated receptor-γ, whose effects were partially different from those on circulating levels of adiponectin. In addition, expression of the genes encoding adiponectin receptor (AdipoR)-1 and AdipoR2 was detected in the rat testis, with developmental changes and gonadotropin regulation for AdipoR2 mRNA, and prominent levels of AdipoR1 in seminiferous tubules. Moreover, recombinant adiponectin significantly inhibited basal and human choriogonadotropin-stimulated testosterone secretion ex vivo, whereas it failed to change relative levels of several Sertoli cell-expressed mRNAs, such as stem cell factor and anti-Müllerian hormone. In summary, our data are the first to document the expression, regulation and functional role of adiponectin in the rat testis. Taken together with its recently reported expression in the ovary and its effects on LH secretion and ovarian steroidogenesis, these results further substantiate a multifaceted role of adiponectin in the control of the reproductive axis, which might operate as endocrine integrator linking metabolism and gonadal function.

CONVERSION OF PROGESTERONE TO ANDROGENS BY NON-FLAGELLATE GERMINAL CELLS ISOLATED FROM SEMINIFEROUS TUBULES OF RAT TESTIS

1974 ◽  
Vol 60 (2) ◽  
pp. 269-NP ◽  
Author(s):  
H. J. GALENA ◽  
C. TERNER
Keyword(s):  
Significant Contribution ◽  
Isotonic Saline ◽  
Interstitial Cells ◽  
Seminiferous Tubules ◽  
Distilled Water ◽  
Rat Testis ◽  
Germinal Cells

SUMMARY A method is described for the isolation of non-flagellate germinal cells of the testis. The interstitial cells were removed by submersion of teased seminiferous tubules in distilled water. The interstitial cells exposed to water burst while the germinal cells inside the tubules remained intact. The tubules were then homogenized in isotonic saline and the non-flagellate germinal cells (spermatocytes and young spermatids) were isolated by centrifugation and filtration through a layer of Sephadex G-25 gel. On incubation with progesterone these cells produced 17α-hydroxyprogesterone, androstenedione, and testosterone. The rate of conversion of progesterone to testosterone in vitro was 0·20 μg/h/109 germinal cells. These results suggest that the non-flagellate germinal cells of the testis may make a significant contribution to the production of androgens.

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