Magnetic activated cell sorting: an effective method for reduction of sperm DNA fragmentation in varicocele men prior to assisted reproductive techniques

Andrologia ◽  
2014 ◽  
pp. n/a-n/a ◽  
Author(s):  
T. Degheidy ◽  
H. Abdelfattah ◽  
A. Seif ◽  
F. K. Albuz ◽  
S. Gazi ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Cell Sorting ◽  
Assisted Reproductive Techniques ◽  
Sperm Dna Fragmentation ◽  
Sperm Dna ◽  
Reproductive Techniques ◽  
Magnetic Activated Cell Sorting

scholarly journals Magnetic-Activated Cell Sorting (MACS): A Useful Sperm-Selection Technique in Cases of High Levels of Sperm DNA Fragmentation

2020 ◽  
Vol 9 (12) ◽  
pp. 3976
Author(s):  
Alberto Pacheco ◽  
Arancha Blanco ◽  
Fernando Bronet ◽  
María Cruz ◽  
Jaime García-Fernández ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Cell Sorting ◽  
Oocyte Donation ◽  
Control Group ◽  
Sperm Dna Fragmentation ◽  
Sperm Selection ◽  
Reproductive Outcomes ◽  
Selection Technique ◽  
Sperm Dna ◽  
Magnetic Activated Cell Sorting

Magnetic-activated cell sorting (MACS) can be used to separate apoptotic sperm with high proportions of fragmented DNA from the rest, thus improving the overall quality of the seminal sample. Therefore, the aim of this retrospective study was to investigate the efficiency of the MACS technique to increase reproductive outcomes in patients with high levels of sperm DNA fragmentation (SDF) undergoing intracytoplasmic sperm-injection (ICSI) cycles. In this study, we analyzed a total of 724 assisted-reproduction-technique (ART) cycles that were divided into two groups: the study group (n = 366) in which the MACS selection technique was performed after density-gradient centrifugation (DGC), and the control group (n = 358) in which only DGC was used for sperm selection. Reproductive outcomes were analyzed in both groups according to three different ART procedures: preimplantation genetic testing for aneuploidy (PGT-A), and autologous and oocyte-donation cycles. The MACS group showed significantly lower miscarriage rates in autologous ICSI cycles, higher pregnancy rates in oocyte-donation cycles, and a significant increase in live-birth rates in both autologous and oocyte-donation cycles. Overall, these results suggested that the MACS technique can be effectively used to eliminate sperm with high SDF levels, and therefore may help to improve reproductive outcomes in couples undergoing ART.

scholarly journals Magnetic-activated cell sorting is not completely effective at reducing sperm DNA fragmentation

2018 ◽  
Vol 35 (12) ◽  
pp. 2215-2221 ◽  
Author(s):  
Mercedes González Martínez ◽  
Pascual Sánchez-Martín ◽  
Mónica Dorado-Silva ◽  
José Luís Fernández ◽  
Estibaliz Girones ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Cell Sorting ◽  
Sperm Dna Fragmentation ◽  
Sperm Dna ◽  
Magnetic Activated Cell Sorting

scholarly journals Magnetic Activated Cell Sorting (MACS) in egg donation cycles and high sperm DNA fragmentation

2014 ◽  
Vol 28 ◽  
pp. S24
Author(s):  
Marta San Celestino Carchenilla ◽  
David Agudo ◽  
Soraya Rubio ◽  
Paloma Simon ◽  
Juan Antonio García Velasco ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Cell Sorting ◽  
Egg Donation ◽  
Sperm Dna Fragmentation ◽  
Sperm Dna ◽  
Magnetic Activated Cell Sorting

Is Magnetic-Activated Cell Sorting an Efficient Technique in Reducing Human Sperm DNA Fragmentation?

2015 ◽  
Vol 21 (S5) ◽  
pp. 63-64
Author(s):  
Sara Bucar ◽  
Ana Gonçalves ◽  
Alberto Barros ◽  
Mário Sousa ◽  
Rosália Sá
Keyword(s):  
Dna Fragmentation ◽  
Cell Sorting ◽  
Human Sperm ◽  
Efficient Technique ◽  
Sperm Dna Fragmentation ◽  
Sperm Dna ◽  
Magnetic Activated Cell Sorting

scholarly journals Healthy baby born after reduction of sperm DNA fragmentation using cell sorting before ICSI

2010 ◽  
Vol 20 (3) ◽  
pp. 320-323 ◽  
Author(s):  
Vanesa Y. Rawe ◽  
Heydy Uriondo Boudri ◽  
Cristian Alvarez Sedó ◽  
Martina Carro ◽  
Sergio Papier ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Cell Sorting ◽  
Sperm Dna Fragmentation ◽  
Healthy Baby ◽  
Sperm Dna
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