Relationship of seminal reactive nitrogen and oxygen species and total antioxidant capacity with sperm DNA fragmentation in infertile couples with normal and abnormal sperm parameters

Andrologia ◽  
2012 ◽  
Vol 46 (1) ◽  
pp. 17-23 ◽  
Author(s):  
F. Khosravi ◽  
M. R. Valojerdi ◽  
M. Amanlou ◽  
L. Karimian ◽  
F. Abolhassani
Keyword(s):  
Antioxidant Capacity ◽  
Dna Fragmentation ◽  
Total Antioxidant Capacity ◽  
Sperm Dna Fragmentation ◽  
Oxygen Species ◽  
Abnormal Sperm ◽  
Sperm Dna ◽  
Total Antioxidant ◽  
Infertile Couples ◽  
Relationship Of

Sperm DNA fragmentation as treatment guidance for infertile couples

2016 ◽  
Vol 106 (3) ◽  
pp. e78-e79 ◽  
Author(s):  
T. Paniza ◽  
T. Cozzubbo ◽  
A. Parrella ◽  
S. Cheung ◽  
M. Goldstein ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Sperm Dna Fragmentation ◽  
Sperm Dna ◽  
Infertile Couples

P–112 Association between dietary total antioxidant capacity and semen quality parameters in male partners of couples attempting fertility

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
G Eslamian ◽  
S Rohani ◽  
N Shoaibinobarian
Keyword(s):  
Antioxidant Capacity ◽  
Dietary Pattern ◽  
Total Antioxidant Capacity ◽  
Semen Quality ◽  
Sperm Count ◽  
Quality Parameters ◽  
Cross Sectional ◽  
Abnormal Sperm ◽  
Total Antioxidant ◽  
Dietary Total Antioxidant Capacity

Abstract Study question Is there any association between dietary total antioxidant capacity (TAC) and semen quality parameters in male partners of couples attempting fertility? Summary answer Greater adherence to diets high in TAC was significantly associated with higher total sperm count, sperm concentration, and sperm motility. What is known already Among multiple factors that affect the etiology of poor semen quality and male infertility, dietary factors have an important contribution. Also, chronic oxidative stress negatively effects semen quality. Whether adherence to the diet rich in antioxidants is associated with better semen quality remains largely unexplored.The concentration of single antioxidant cannot show the total antioxidant power of the diet, therefore the concept of dietary total antioxidant capacity (TAC) was invented.Dietary scores are useful approach to evaluate the degree of adherence to specific dietary pattern and its benefits in regard to health Study design, size, duration This was a cross-sectional study of 350 men from couples attending a fertility center in Tehran, Iran, recruited between June 2015 and September 2019. Men aged 25–50 years with complete dietary data were analyzed. Participants/materials, setting, methods Diet was assessed via a reproducible and valid 168-item semi-quantitative food frequency questionnaire to determine the entire antioxidants of the usual diet in order to calculate dietary TAC. Semen quality was assessed according to World Health Organization 2010 guidelines. The association between dietary TAC and semen parameters were assessed adjusting for potential confounders through multiple logistic regression analysis. Main results and the role of chance The average age of study participants was 34.9 ± 7.6 years and their BMI was 28.5 ± 4.3 kg/m2. Participants in the lower tertile of the dietary TAC were lower educated (p < 0.01), more physically active (p < 0.05), and predominantly had abnormal sperm progressive motility (p < 0.01). In the multivariable adjusted models, men in the lowest tertile of the dietary TAC Score had 2.9 times higher likelihood of having abnormal sperm motility, total sperm count and concentration, compared to men in the highest tertile of the TAC score (p < 0.05). Limitations, reasons for caution The main limitation of our study was its cross-sectional design, limiting our ability to derive causal association. Wider implications of the findings: Our study suggests that dietary pattern comprising mainly of antioxidant nutrients may help improve semen quality. Our results are consistent with previous studies showing that plant-based diet contains higher levels of antioxidants are associated with better measures of semen quality. Trial registration number Not applicable

scholarly journals DNA Fragmentation in Viable and Non-Viable Spermatozoa Discriminates Fertile and Subfertile Subjects with Similar Accuracy

2020 ◽  
Vol 9 (5) ◽  
pp. 1341
Author(s):  
Monica Muratori ◽  
Giulia Pellegrino ◽  
Giusi Mangone ◽  
Chiara Azzari ◽  
Francesco Lotti ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Semen Quality ◽  
Semen Parameters ◽  
Sperm Dna Fragmentation ◽  
Viable Cells ◽  
Sperm Dna ◽  
Infertile Couples ◽  
Natural Conception ◽  
Similar Accuracy ◽  
Sperm Population

Sperm DNA fragmentation (sDF) negatively affects reproduction and is traditionally detected in total sperm population including viable and non-viable spermatozoa. Here, we aimed at exploring the ability of DNA fragmentation to discriminate fertile and subfertile men when detected in viable (viable sDF), non-viable (non-viable sDF), and total spermatozoa (total sDF). We revealed sDF in 91 male partners of infertile couples and 71 fertile men (max 1 year from natural conception) with LiveTUNEL coupled to flow cytometry, able to reveal simultaneously DNA fragmentation and cell viability. We found that the three sDF parameters discriminated fertile and subfertile men with similar accuracy and independently from age and basal semen parameters: AUCs (area under the curves) (95% CI) were: 0.696 (0.615–0.776), p < 0.001 for total sDF; 0.718 (0.640–0.797), p < 0.001 for viable sDF; 0.760 (0.685–0.835), p < 0.001 for non-viable sDF. We also found that total and non-viable but not viable sDF significantly correlated to age and semen quality. In conclusion, the three sDF parameters similarly discriminated fertile and subfertile men. Viable spermatozoa with DNA fragmentation are likely cells able to fertilize the oocyte but failing to properly support subsequent embryo development. Non-viable sDF could be a sign of a subtler damage extended beyond the non-viable cells.

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