Tissue eosinophils express the IL‐33 receptor ST2 and type 2 cytokines in patients with eosinophilic esophagitis

Maintenance of Type 2 Response by CXCR6-Deficient ILC2 in Papain-Induced Lung Inflammation

International Journal of Molecular Sciences ◽

10.3390/ijms20215493 ◽

2019 ◽

Vol 20 (21) ◽

pp. 5493 ◽

Cited By ~ 1

Author(s):

Meunier ◽

Chea ◽

Garrido ◽

Perchet ◽

Petit ◽

...

Keyword(s):

Immune Response ◽

Immune Responses ◽

Nk Cell ◽

Lymphoid Cells ◽

Inflammatory Conditions ◽

Airway Diseases ◽

Type 2 Cytokines ◽

Lymphoid Organogenesis ◽

Deficient Mice

Innate lymphoid cells (ILC) are important players of early immune defenses in situations like lymphoid organogenesis or in case of immune response to inflammation, infection and cancer. Th1 and Th2 antagonism is crucial for the regulation of immune responses, however mechanisms are still unclear for ILC functions. ILC2 and NK cells were reported to be both involved in allergic airway diseases and were shown to be able to interplay in the regulation of the immune response. CXCR6 is a common chemokine receptor expressed by all ILC, and its deficiency affects ILC2 and ILC1/NK cell numbers and functions in lungs in both steady-state and inflammatory conditions. We determined that the absence of a specific ILC2 KLRG1+ST2– subset in CXCR6-deficient mice is probably dependent on CXCR6 for its recruitment to the lung under inflammation. We show that despite their decreased numbers, lung CXCR6-deficient ILC2 are even more activated cells producing large amount of type 2 cytokines that could drive eosinophilia. This is strongly associated to the decrease of the lung Th1 response in CXCR6-deficient mice.

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A case of eosinophilic esophagitis with autoimmune polyendocrine syndrome type 2, including autoimmune gastritis

Clinical Journal of Gastroenterology ◽

10.1007/s12328-020-01333-y ◽

2021 ◽

Author(s):

Maki Ayaki ◽

Noriaki Manabe ◽

Minoru Fujita ◽

Jun Nakamura ◽

Aya Sunago ◽

...

Keyword(s):

Eosinophilic Esophagitis ◽

Autoimmune Gastritis ◽

Syndrome Type ◽

Autoimmune Polyendocrine Syndrome

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Type-1 and Type-2 Cytokines in Human Decidual Tissue and Trophoblasts from Normal and Abnormal Pregnancies Detected by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)

American Journal of Reproductive Immunology ◽

10.1111/j.1600-0897.1999.tb00113.x ◽

1999 ◽

Vol 42 (6) ◽

pp. 361-368 ◽

Cited By ~ 27

Author(s):

Angels Vives ◽

Juan Balasch ◽

Jordi Yagüe ◽

Llorenç Quintó ◽

Jaume Ordi ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Reverse Transcriptase ◽

Rt Pcr ◽

Chain Reaction ◽

Decidual Tissue ◽

Type 2 Cytokines ◽

Polymerase Chain

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Regulation of bronchial epithelial barrier integrity by type 2 cytokines and histone deacetylases in asthmatic patients

Journal of Allergy and Clinical Immunology ◽

10.1016/j.jaci.2016.03.050 ◽

2017 ◽

Vol 139 (1) ◽

pp. 93-103 ◽

Cited By ~ 69

Author(s):

Paulina Wawrzyniak ◽

Marcin Wawrzyniak ◽

Kerstin Wanke ◽

Milena Sokolowska ◽

Kreso Bendelja ◽

...

Keyword(s):

Histone Deacetylases ◽

Epithelial Barrier ◽

Bronchial Epithelial ◽

Barrier Integrity ◽

Asthmatic Patients ◽

Type 2 Cytokines

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A type 2 cytokine axis for thymus emigration

Journal of Experimental Medicine ◽

10.1084/jem.20170271 ◽

2017 ◽

Vol 214 (8) ◽

pp. 2205-2216 ◽

Cited By ~ 20

Author(s):

Andrea J. White ◽

Song Baik ◽

Sonia M. Parnell ◽

Amanda M. Holland ◽

Frank Brombacher ◽

...

Keyword(s):

T Cells ◽

Stromal Cells ◽

Perivascular Spaces ◽

Thymocyte Development ◽

Invariant Nkt Cells ◽

Developmental Program ◽

Innate T Cells ◽

Type 2 Cytokines ◽

Thymic Stromal Cells

In the thymus, stromal microenvironments support a developmental program that generates mature T cells ready for thymic exit. The cellular and molecular specialization within thymic stromal cells that enables their regulation of specific stages of thymocyte development is poorly understood. Here, we show the thymic microenvironment expresses the type 2 IL-4R complex and is functionally responsive to its known ligands, IL-4 and IL-13. Absence of IL-4Rα limits thymocyte emigration, leading to an intrathymic accumulation of mature thymocytes within medullary perivascular spaces and reduced numbers of recent thymic emigrants. Thymus transplantation shows this requirement maps to IL-4Rα expression by stromal cells, and we provide evidence that it regulates thymic exit via a process distinct from S1P-mediated migration. Finally, we reveal a cellular mechanism by which IL-4+IL-13+ invariant NKT cells are necessary for IL-4Rα signaling that regulates thymic exit. Collectively, we define a new axis for thymic emigration involving stimulation of the thymic microenvironment via type 2 cytokines from innate T cells.

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Distinct Type 2-high Inflammation Associated Molecular Signatures of Chronic Rhinosinusitis with Nasal Polyps with Comorbid Asthma

10.21203/rs.3.rs-16929/v2 ◽

2020 ◽

Author(s):

Ming Wang ◽

Xiangting Bu ◽

Ge Luan ◽

Liqing Lin ◽

Yang Wang ◽

...

Keyword(s):

Arachidonic Acid ◽

Nasal Polyps ◽

Acid Metabolism ◽

Distinct Type ◽

Arachidonic Acid Metabolism ◽

Eosinophil Infiltration ◽

Phenotypic Traits ◽

Molecular Signatures ◽

Type 2 Cytokines

Abstract Background: Patients with chronic rhinosinusitis with nasal polyps (CRSwNP) and comorbid asthma have more severe disease and are difficult to treat. However, the molecular endotypes associated with CRSwNP with comorbid asthma (CRSwNP+AS) are not clear. This study aimed to investigate the characteristics of type 2 inflammation and the molecular signatures associated with CRSwNP+AS. Methods: A total of 195 subjects; including 65 CRSwNP+AS patients, 99 patients with CRSwNP-alone, and 31 healthy control subjects; were enrolled in the study. Nasal tissues from patients with CRSwNP+AS, CRSwNP-alone and control subjects were assessed for infiltration of inflammatory cells and concentrations of total IgE. Whole-transcriptome sequencing was performed and differentially expressed (DE) mRNAs and long non-coding RNAs (lncRNAs) and their associated pathways were analyzed. The correlations between type 2 cytokines and local eosinophils, tissue IgE, and transcriptome signatures were evaluated. Results: Significantly higher local eosinophil infiltration and higher levels of total IgE were found in nasal tissues from CRSwNP+AS patients than in nasal tissues from CRSwNP-alone patients. Furthermore, atopy and recurrence were significantly more frequent in patients with CRSwNP+AS than in patients with CRSwNP-alone (62.5% vs 28.6% and 66.7% vs 26.9%, respectively). RNA sequencing analysis identified 1988 common DE-mRNAs, and 176 common DE-lncRNAs shared by CRSwNP+AS versus control and CRSwNP-alone versus control. Weighted gene coexpression network analysis (WGCNA) identified LINC01146 as hub lncRNA dysregulated in both subtypes of CRSwNP. Overall, 968 DE-mRNAs and 312 DE-lncRNAs were identified between CRSwNP+AS and CRSwNP-alone. Both pathway enrichment analysis and WGCNA indicated that the phenotypic traits of CRSwNP+AS were mainly associated with higher activities of arachidonic acid metabolism, type 2 cytokines related pathway and fibrinolysis pathway, and lower activity of IL-17 signalling pathway. Furthermore, the expression of type 2 cytokines; IL5 and IL13, was positively correlated with local eosinophil infiltration, tissue IgE level, and the expression of DE-mRNAs that related to arachidonic acid metabolism. Moreover, WGCNA identified HK3-006 as hub lncRNA in yellow module that most positively correlated with phenotypic traits of CRSwNP+AS. Conclusions: Patients with CRSwNP+AS have distinct type 2-high inflammation-associated molecular signatures in nasal tissues compared to patients with CRSwNP-alone.

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