Gibberellin‐Regulated Protein sensitization in Japanese cedar ( Cryptomeria japonica ) pollen allergic Japanese cohorts

Allergy ◽  
2021 ◽  
Author(s):  
Tomona Iizuka ◽  
Mari Takei ◽  
Yukiko Saito ◽  
Farhana Rumi ◽  
Jingkang Zheng ◽  
...  
Keyword(s):  
Cryptomeria Japonica ◽  
Japanese Cedar ◽  
Protein Sensitization

scholarly journals CRISPR/Cas9-mediated targeted mutagenesis in Japanese cedar (Cryptomeria japonica D. Don)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yoshihiko Nanasato ◽  
Masafumi Mikami ◽  
Norihiro Futamura ◽  
Masaki Endo ◽  
Mitsuru Nishiguchi ◽  
...  
Keyword(s):  
Genome Editing ◽  
Cryptomeria Japonica ◽  
Fluorescent Protein ◽  
Japanese Cedar ◽  
Targeted Mutagenesis ◽  
Embryogenic Tissue ◽  
Breeding Period ◽  
Single Mutation ◽  
Knock Out ◽  
Guide Rna

AbstractCryptomeria japonica (Japanese cedar or sugi) is one of the most important coniferous tree species in Japan and breeding programs for this species have been launched since 1950s. Genome editing technology can be used to shorten the breeding period. In this study, we performed targeted mutagenesis using the CRISPR/Cas9 system in C. japonica. First, the CRISPR/Cas9 system was tested using green fluorescent protein (GFP)-expressing transgenic embryogenic tissue lines. Knock-out efficiency of GFP ranged from 3.1 to 41.4% depending on U6 promoters and target sequences. The GFP knock-out region was mottled in many lines, indicating genome editing in individual cells. However, in 101 of 102 mutated individuals (> 99%) from 6 GFP knock-out lines, embryos had a single mutation pattern. Next, we knocked out the endogenous C. japonica magnesium chelatase subunit I (CjChlI) gene using two guide RNA targets. Green, pale green, and albino phenotypes were obtained in the gene-edited cell lines. Sequence analysis revealed random deletions, insertions, and replacements in the target region. Thus, targeted mutagenesis using the CRISPR/Cas9 system can be used to modify the C. japonica genome.

scholarly journals Japanese Cedar (Cryptomeria japonica) Pollinosis in Jeju, Korea: Is It Increasing?

2015 ◽  
Vol 7 (3) ◽  
pp. 295 ◽  
Author(s):  
Jaechun Lee ◽  
Keun Hwa Lee ◽  
Hye-Sook Lee ◽  
Sung-Chul Hong ◽  
Jeong Hong Kim
Keyword(s):  
Cryptomeria Japonica ◽  
Japanese Cedar

Spatiotemporal variation in N2O flux within a slope in a Japanese cedar (Cryptomeria japonica) forest

2009 ◽  
Vol 96 (1-3) ◽  
pp. 163-175 ◽  
Author(s):  
Kazuya Nishina ◽  
Chisato Takenaka ◽  
Shigehiro Ishizuka
Keyword(s):  
Cryptomeria Japonica ◽  
Japanese Cedar ◽  
Spatiotemporal Variation ◽  
N2o Flux
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